Structural transitions and rheological properties of poly-d-lysine hydrobromide: effect of pH, salt, temperature, and shear rate
-
Héla Khemissi
Abstract
In this work, we analyzed the pH, temperature, and salt effects of the charged polypeptide and its size, poly-d-lysine (PDL) molecules while applying dynamic light scattering (DLS), Zeta potential, and rheology techniques to assess the most important characteristics of PDL. The experimental results showed that the structural transitions of PDL were a result of a competition between electrostatic interaction, which promotes an extended state, and the hydrophobic effect, which favors a compact state. Moreover, by exploiting the electrokinetic charge on the PDL molecules the zeta potential was determined. We tried to find an analogy between size, viscosity, and conformational changes of PDL so to serve as a guide for polypeptide aggregation in solution.
-
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
-
Research funding: None declared.
-
Conflict of interest statement: The authors declare no conflicts of interest regarding this article.
References
1. Ryser, H. J., Shen, W. C. Conjugation of methotrexate to poly (L-lysine) increases drug transport and overcomes drug resistance in cultured cells. Proc. Natl. Acad. Sci. U. S. A. 1978, 75, 3867–3870; https://doi.org/10.1073/pnas.75.8.3867.Search in Google Scholar PubMed PubMed Central
2. Maestrelli, P., Saetta, M., Di Stefano, A., Calcagni, P. G., Turato, G., Ruggieri, M. P., Roggeri, A., Mapp, C. E., Fabbri, L. M. Comparison of leukocyte counts in sputum, bronchial biopsies, and bronchoalveolar lavage. Am. J. Respir. Crit. Care Med. 1995, 152, 1926–1931; https://doi.org/10.1164/ajrccm.152.6.8520757.Search in Google Scholar PubMed
3. Chiou, R. Y. Antioxidative activity in oils prepared from peanut kernels subjected to various treatments and roasting. J. Agric. Food Chem. 1992, 40, 1958–1962; https://doi.org/10.1021/jf00022a046.Search in Google Scholar
4. Chittchang, M., Alur, H. H., Mitra, A. K., Johnston, T. P. Poly (L-lysine) as a model drug macromolecule with which to investigate secondary structure and membrane transport, part I: physicochemical and stability studies. J. Pharm. Pharmacol. 2002, 54, 315–523; https://doi.org/10.1211/0022357021778556.Search in Google Scholar PubMed
5. Riseman, J., Kirkwood, J. G. The intrinsic viscosity, translational and rotatory diffusion constants of rod-like macromolecules in solution. J. Chem. Phys. 1950, 18, 512–516; https://doi.org/10.1063/1.1747672.Search in Google Scholar
6. van der Zande, B. M., Dhont, J. K., Böhmer, M. R., Philipse, A. P. Colloidal dispersions of gold rods characterized by dynamic light scattering and electrophoresis. Langmuir 2000, 25, 459–464; https://doi.org/10.1021/la990043x.Search in Google Scholar
7. Rodriguez-Maldonado, L., Fernandez-Nieves, A., Fernandez-Barbero, A. Dynamic light scattering from high molecular weight poly-L-lysine molecules. Colloids Surf. A Physicochem. Eng. Asp. 2005, 270, 335–339; https://doi.org/10.1016/j.colsurfa.2005.09.003.Search in Google Scholar
8. Nicolai, T., Gimel, J., Johnsen, R. Analysis of relaxation functions characterized by a broad monomodal relaxation time distribution. J. Phys. II EDP Sci. 1996, 6, 697–711; https://doi.org/10.1051/jp2:1996206.10.1051/jp2:1996206Search in Google Scholar
9. Greenfield, N. J., Fasman, G. D. Computed circular dichroism spectra for the evaluation of protein conformation. Biochemistry 1969, 8, 4108–4116; https://doi.org/10.1021/bi00838a031.Search in Google Scholar PubMed
10. Chittchang, M., Salamat‐Miller, N., Alur, H. H., Velde, D. G., Mitra, A. K., Johnston, T. P. Poly (L-lysine) as a model drug macromolecule with which to investigate secondary structure and microporous membrane transport, part 2: diffusion studies. J. Pharm. Pharmacol. 2002, 54, 1497–1505; https://doi.org/10.1211/002235702108.Search in Google Scholar PubMed
11. Wu, Y., Zhang, D., Ma, P., Zhou, R., Hua, L., Liu, R. Lithium hexamethyldisilazide initiated superfast ring opening polymerization of alpha-amino acid N-carboxyanhydrides. Nat. Commun. 2018, 9, 1–10; https://doi.org/10.1038/s41467-018-07711-y.Search in Google Scholar PubMed PubMed Central
12. Daoust, H., St-Pierre, S. L’ effet de la longueur de la chaîne et du p H sur la conformation de polyampholytes alternés du type (l-lysyl-l-aspartyl) n. Can. J. Chem. 1975, 53, 1861–1871; https://doi.org/10.1139/v75-261.Search in Google Scholar
13. Wang, Y., Chang, Y. C. Synthesis and conformational transition of surface-tethered polypeptide: poly (L-lysine). Macromolecules 2003, 36, 6511–6518; https://doi.org/10.1021/ma034093r.Search in Google Scholar
14. Tiffany, M. L., Krimm, S. New chain conformations of poly (glutamic acid) and polylysine. Biopolymers 1968, 6, 1379–1382; https://doi.org/10.1002/bip.1968.360060911.Search in Google Scholar PubMed
15. Lin, H., Dass, C. Conformational changes in β-endorphin as studied by electrospray ionization mass spectrometry. Rapid Commun. Mass Spectrom. 2001, 15, 2341–2346; https://doi.org/10.1002/rcm.513.Search in Google Scholar PubMed
16. Brandts, J. F. The thermodynamics of protein denaturation. II. A model of reversible denaturation and interpretations regarding the stability of chymotrypsinogen. J. Am. Chem. Soc. 1964, 86, 4302–4314; https://doi.org/10.1021/ja01074a014.Search in Google Scholar
17. Nel, A. E., Mädler, L., Velegol, D., Xia, T., Hoek, E. M., Somasundaran, P., Klaessig, F., Castranova, V., Thompson, M. Understanding biophysicochemical interactions at the nano–bio interface. Nat. Mater. 2009, 8, 543–557; https://doi.org/10.1038/nmat2442.Search in Google Scholar PubMed
18. Burda, C., Chen, X., Narayanan, R., El-Sayed, M. A. Chemistry and properties of nanocrystals of different shapes. Chem. Rev. 2005, 105, 1025–1029; https://doi.org/10.1021/cr030063a.Search in Google Scholar PubMed
19. Mills, N. ChemDraw Ultra 10.0. J. Am. Chem. Soc. 2006, 128, 13649–13650; https://doi.org/10.1021/ja0697875.Search in Google Scholar
20. Satake, I., Yang, J. T. Effect of temperature and pH on the β–helix transition of poly (l-lysine) in sodium dodecyl sulfate solution. Biopolymers 1975, 14, 1841–1846; https://doi.org/10.1002/bip.1975.360140906.Search in Google Scholar
21. Lin, S. C., Lee, W. I., Schurr, J. M. Brownian motion of highly charged poly (l-lysine). Effects of salt and polyion concentration. Biopolymers 1978, 17, 1041–1064; https://doi.org/10.1002/bip.1978.360170418.Search in Google Scholar
22. Volk, N., Vollmer, D., Schmidt, M., Oppermann, W., Huber, K. Conformation and Phase Diagrams of Flexible Polyelectrolytes. Polyelectrolytes with Defined Molecular Architecture II; Springer: London, 2004; pp. 29–65.10.1007/b11348Search in Google Scholar
23. Dobrynin, A. V., Colby, R. H., Rubinstein, M. Scaling theory of polyelectrolyte solutions. Macromolecules 1995, 28, 1859–1871; https://doi.org/10.1021/ma00110a021.Search in Google Scholar
24. Muthukumar, M. Dynamics of polyelectrolyte solutions. J. Chem. Phys. 1997, 107, 2619–2635; https://doi.org/10.1063/1.474573.Search in Google Scholar
25. Arslan, E., Yener, M. E., Esin, A. Rheological characterization of tahin/pekmez (sesame paste/concentrated grape juice) blends. J. Food Eng. 2005, 69, 167–172; https://doi.org/10.1016/j.jfoodeng.2004.08.010.Search in Google Scholar
26. Santos, P. H., da Silva, L. H. M., da Cruz Rodrigues, A. M., de Souza, J. A. R. Influence of temperature, concentration and shear rate on the rheological behavior of Malay apple (Syzygium malaccense) juice. Braz. J. Food Technol. 2016, 19, 1–9; https://doi.org/10.1590/1981-6723.0915.Search in Google Scholar
27. Carreau, P. J., Lavoie, P. A., Yziquel, F. Rheological properties of concentrated suspensions. In Advances in the Flow and Rheology of Non-Newtonian Fluids: Part A; Siginer, D. A., Kee, D., Chabra, R. P., Eds. Elsevier: New York, 1999, pp. 1299–1345.10.1016/S0169-3107(99)80019-7Search in Google Scholar
28. Lu, J., Luo, Z., Xiao, Z. Effect of lysine and glycine on pasting and rheological properties of maize starch. Food Res. Int. 2012, 49, 612–617; https://doi.org/10.1016/j.foodres.2012.06.038.Search in Google Scholar
© 2022 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Material Properties
- Development and characterization of eco-friendly biopolymer gellan gum based electrolyte for electrochemical application
- Structural transitions and rheological properties of poly-d-lysine hydrobromide: effect of pH, salt, temperature, and shear rate
- Carbon dioxide adsorption onto modified polyvinyl chloride with ionic liquid
- Synergistic effect of organic-Zn(H2PO2)2 and lithium containing polyhedral oligomeric phenyl silse-squioxane on flame-retardant, thermal and mechanical properties of poly(ethylene terephthalate)
- Preparation and Assembly
- Network structural hardening of polypropylene matrix using hybrid of 0D, 1D and 2D carbon-ceramic nanoparticles with enhanced mechanical and thermomechanical properties
- An environment friendly hemp fiber modified with phytic acid for enhancing fire safety of automobile parts
- Flexible silicone rubber/carbon fiber/nano-diamond composites with enhanced thermal conductivity via reducing the interface thermal resistance
- In situ synthesis of Ag NPs in the galactomannan based biodegradable composite for the development of active packaging films
- Engineering and Processing
- Multi-objective optimization of injection molded parts with insert based on IFOA-GRNN-NSGA-II
Articles in the same Issue
- Frontmatter
- Material Properties
- Development and characterization of eco-friendly biopolymer gellan gum based electrolyte for electrochemical application
- Structural transitions and rheological properties of poly-d-lysine hydrobromide: effect of pH, salt, temperature, and shear rate
- Carbon dioxide adsorption onto modified polyvinyl chloride with ionic liquid
- Synergistic effect of organic-Zn(H2PO2)2 and lithium containing polyhedral oligomeric phenyl silse-squioxane on flame-retardant, thermal and mechanical properties of poly(ethylene terephthalate)
- Preparation and Assembly
- Network structural hardening of polypropylene matrix using hybrid of 0D, 1D and 2D carbon-ceramic nanoparticles with enhanced mechanical and thermomechanical properties
- An environment friendly hemp fiber modified with phytic acid for enhancing fire safety of automobile parts
- Flexible silicone rubber/carbon fiber/nano-diamond composites with enhanced thermal conductivity via reducing the interface thermal resistance
- In situ synthesis of Ag NPs in the galactomannan based biodegradable composite for the development of active packaging films
- Engineering and Processing
- Multi-objective optimization of injection molded parts with insert based on IFOA-GRNN-NSGA-II
