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Development of a polystyrene latex-based reagent for rheumatoid factor detection

  • Grisel Marrero , Lilian P. Delgado , Humberto Caroll , Neise Ortiz , Alexis Musacchio and Tamara Menendez EMAIL logo
Published/Copyright: July 24, 2015
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Journal of Polymer Engineering
From the journal Journal of Polymer Engineering Volume 36 Issue 3

Abstract

A rapid, specific and sensible polystyrene latex-based reagent has been developed to detect rheumatoid factor (RF) in human sera. Detection of RF is one of the criteria for rheumatoid arthritis (RA) diagnosis. RF includes immunoglobulins IgG, IgM or IgA targeting human-IgG Fc region, found in sera of 70–90% of patients with RA. Spherical, clean polystyrene particles of 480±80 nm with a Z-potential of -28 mV were synthesized. Purified gamma-globulin human blood fraction, used as IgG source, was adsorbed on particles in glycine-buffered saline with a fivefold excess of the immunoglobulins needed to saturate the calculated total surface area of the particles. The reagent was calibrated against the World Health Organization international serum reference preparation and tested with commercial positive and negative RF controls. Reactivity of the developed diagnostic reagent with human sera, either positive or negative for RF, was evaluated. The sera were tested both intact and following complement inactivation. When complement-inactivated sera were used, our reagent showed 100% coincidence of results with a commercial diagnostic kit of specificity of 80% and sensibility of 93%. The in-house reagent developed in the present work is easy to prepare and at relatively low cost, making it ideal for RF detection in low-income settings.

Keywords: disease diagnosis; latex; polystyrene; rheumatoid arthritis; rheumatoid factor
Corresponding author: Tamara Menendez, Center of Biomaterials (BIOMAT), University of Havana, Ave. Universidad, entre Ronda y G, Plaza de la Revolucion, Havana, Cuba, e-mail:

Acknowledgments

The authors thank Dr. JG Arrieta (Havana, Cuba) for the critical reading of the original manuscript.

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Received: 2015-5-28
Accepted: 2015-6-11
Published Online: 2015-7-24
Published in Print: 2016-4-1

©2016 by De Gruyter

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  5. Development of a polystyrene latex-based reagent for rheumatoid factor detection
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https://doi.org/10.1515/polyeng-2015-0238
Keywords for this article
disease diagnosis; latex; polystyrene; rheumatoid arthritis; rheumatoid factor

Articles in the same Issue

  1. Frontmatter
  2. Review
  3. The role of poly(acrylic acid) in conventional glass polyalkenoate cements
  4. Original articles
  5. Development of a polystyrene latex-based reagent for rheumatoid factor detection
  6. Biodegradation study of bio-corn flour filled low density polyethylene composites assessed by natural soil
  7. Efficacy of PU foam materials for scientific investigation in footwear research
  8. Degradation of PVDF-based composite membrane and its impacts on membrane intrinsic and separation properties
  9. Fabrication of cellulose fine fiber based membranes embedded with silver nanoparticles via Forcespinning
  10. Influence of wax content on the electrical, thermal and tribological behaviour of a polyamide 6/graphite composite
  11. In situ formation of copper nanoparticles in a p(NIPAM-VAA-AAm) terpolymer microgel that retains the swelling behavior of microgels
  12. Possible application of lead sulfide quantum dot in memory device
  13. Solution properties of polyaniline/carbon particle composites
  14. Preparation and characterization of SiO2/fluoroalkyl-trialkoxysilane/2-hydroxyethyl methacrylate/trimethylolpropane triacrylate and SiO2/3-(trimethoxysilyl)propyl methacrylate/ 2-hydroxyethyl methacrylate/trimethylolpropane triacrylate coatings on glass substrates using the sol-gel method
  15. The performance and morphology of PMMA/SAN/ABS blends
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