The effect of chemical modification using citraconic anhydride on the stability of α-amylase from Aspergillus fumigatus
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Yandri Yandri
,
Lupia Widya Astuti
Abstract
The purpose of this research is to improve the stability of α-amylase from Aspergillus fumigatus by chemical modification with citraconic anhydride. The α-amylase was isolated using a centrifugation technique, followed by purification using precipitation and dialysis of ammonium sulfate salt. The experimental results demonstrate that the purity of purified α-amylase is 13.41 times higher than that of the crude extract. A significant increase in the optimum temperature was also achieved, in which the optimum temperature of 50 °C was found for native α-amylase, while for modified α-amylase, the optimum temperature of 60 °C was found. Similarly, an increase in half-life was also evident, which is 38.72 min found for the native enzyme to 256.67–330.00 min for modified α-amylase, depending on the volume of citraconic anhydride used. Modification also resulted in increased free energy values (ΔG i) from 104.348 for the native enzyme to 109.585–110.281 kJ mol−1 for modified α-amylase, indicating that modified α-amylase is stiffer than native α-amylase. The results obtained in this work demonstrate that citric anhydride is a very promising modifying agent to improve the stability and performance of α-amylase enzyme isolated from A. fumigatus. The findings of this study also offer an opportunity for the application of citric anhydride for other enzymes.
Funding source: Direktorat Jenderal Pendidikan Tinggi
Award Identifier / Grant number: 027/E5/PG.02.00.PL/2023
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Research funding: The authors are grateful to the Ministry of Education, Culture, Research and Technology for funding this study based on Basic Research 2023 with contract numbers 027/E5/PG.02.00.PL/2023 on 12 April 2023 and 2192/UN26.21/PN/2023 on 17 April 2023.
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Artikel in diesem Heft
- Frontmatter
- In this issue
- Special topic papers
- Ozone-initiated degradation of 1,2-dichlorobenzene over ceria-supported manganese, nickel, vanadium and iron catalysts
- The effect of chemical modification using citraconic anhydride on the stability of α-amylase from Aspergillus fumigatus
- Decarbonizing our environment via the promotion of biomass methanation in developing nations: a waste management tool
- Maximising the consistency of the presentation of the molecular level with its quantum mechanical description: challenges and opportunities
- The amide group and its preparation methods by acid-amine coupling reactions: an overview
- Surface tension measurement of FAP-based ionic liquid pendant drops in a high vacuum/gas cell
- Investigating the bioactive compounds from Capsicum annum as a probable alternative therapy for prostate cancer treatment: a structure-based drug design approach
- Crucial role of the internalisation of the distinction between dependent and independent variables for clearer chemistry understanding
- 3-(4-methoxyphenyl) acrylic acid halts redox imbalance and modulate purinergic enzyme activity in iron-induced testicular injury
- Computational study on reactivity, aromaticity, and absorption spectra of chrysene: effect of BN doping and substituents
- Health benefit of lipid composition of orange (Citrus sinensis) fruit pulp at different maturation stages
Artikel in diesem Heft
- Frontmatter
- In this issue
- Special topic papers
- Ozone-initiated degradation of 1,2-dichlorobenzene over ceria-supported manganese, nickel, vanadium and iron catalysts
- The effect of chemical modification using citraconic anhydride on the stability of α-amylase from Aspergillus fumigatus
- Decarbonizing our environment via the promotion of biomass methanation in developing nations: a waste management tool
- Maximising the consistency of the presentation of the molecular level with its quantum mechanical description: challenges and opportunities
- The amide group and its preparation methods by acid-amine coupling reactions: an overview
- Surface tension measurement of FAP-based ionic liquid pendant drops in a high vacuum/gas cell
- Investigating the bioactive compounds from Capsicum annum as a probable alternative therapy for prostate cancer treatment: a structure-based drug design approach
- Crucial role of the internalisation of the distinction between dependent and independent variables for clearer chemistry understanding
- 3-(4-methoxyphenyl) acrylic acid halts redox imbalance and modulate purinergic enzyme activity in iron-induced testicular injury
- Computational study on reactivity, aromaticity, and absorption spectra of chrysene: effect of BN doping and substituents
- Health benefit of lipid composition of orange (Citrus sinensis) fruit pulp at different maturation stages
