Evaluation of in vitro antioxidant activities, total phenolic and elemental contents of common herbs and spices (Moringa oleifera leaves, Allium sativum (Garlic) and Momordica charantia (ejinrin) leaves) in South-West Nigeria

Bukunola Oluyemisi Adegbesan; Esther Nkechi Ezima; Basit Opeyemi Hassan; Jeremiah Oluwasegun Kehinde; Aderinsola Ayoyemi Adewale; Ifabunmi Oduyemi Osonuga; Samuel Oluwadare Olalekan

doi:10.1515/pac-2023-1128

Article

Evaluation of in vitro antioxidant activities, total phenolic and elemental contents of common herbs and spices (Moringa oleifera leaves, Allium sativum (Garlic) and Momordica charantia (ejinrin) leaves) in South-West Nigeria

Bukunola Oluyemisi Adegbesan , Esther Nkechi Ezima , Basit Opeyemi Hassan , Jeremiah Oluwasegun Kehinde , Aderinsola Ayoyemi Adewale , Ifabunmi Oduyemi Osonuga and Samuel Oluwadare Olalekan

Published/Copyright: May 8, 2024

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From the journal Pure and Applied Chemistry Volume 96 Issue 10

Abstract

Natural remedies are being widely utilized in some disease’s conditions including cancer, diabetes, neurodegenerative diseases, atherosclerosis hypertension and other cardiovascular diseases. The therapeutic intervention of medicinal plants and spices cannot be overemphasized in the management and control of diseases but the exact modes of action of these herbs and plants have not been fully elucidated. This research work was designed to study the expression of selected elements, zinc (Zn), iron (Fe), copper (Cu), selenium (Se), lead (Pb) and cadmium (Cd); the phytochemical and the in vitro antioxidant properties of ethanolic extracts of Moringa oleifera leaves, Allium sativum (Garlic) and Momordica charantia (ejinrin) leaves for their potential involvement in the prevention and management of cardiovascular diseases and cancer through different chemical methods. In vitro antioxidant properties were assayed by investigating 2,2-diphenyl-2-picryl-hydrazyl (DPPH) free radicals scavenging potentials and Ferric Reducing Antioxidant Potential (FRAP); total phenolic content was determined by using Folin-Ciocalteu assay and the elemental contents of these extracts was investigated through the use of Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). All tests were run in triplicates and analysed. Our results revealed that the ethanolic extracts of M. oleifera leaves, A. sativum (Garlic) and M. charantia (ejinrin) leaves possess significant antioxidant activities and express important and beneficial elements (in marginal and trace amounts) and phytochemicals. These results suggest that the medicinal attributes of these plants may be linked to the radical scavenging abilities, beneficial elements and phytochemicals expression of their ethanolic extracts which may likely be a good direction in the area of drug discovery and development.

Keywords: Allium sativum ; antioxidants; minerals; Momordica charantia ; Moringa oleifera ; phytochemistry; VCCA-2023

Corresponding author: Bukunola Oluyemisi Adegbesan, Department of Biochemistry, Faculty of Basic Medical Sciences, Obafemi Awolowo College of Health Sciences, Olabisi Onabanjo University, Sagamu Campus, Sagamu, Ogun, Nigeria, e-mail: adegbesan.bukunola@oouagoiwoye.edu.ng

Article note: A collection of invited papers based on presentations at the Virtual Conference on Chemistry and its Applications 2023.

Acknowledgments

The authors will like to appreciate Dr J. S Ashidi of the department of plant science, Olabisi Onabanjo University, Ogun State Nigeria for his guide and expertise on plant identification and extraction. The authors will also like to appreciate Prof. Ademuyiwa Oladipo of the department of Biochemistry, Federal University of Agriculture, Abeokuta (FUNAAB) for his support on the elemental analysis.

Research funding: The authors declare that no funds, grants, or other support were received during the preparation of this manuscript.

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Published Online: 2024-05-08

Published in Print: 2024-10-28

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Articles in the same Issue

https://doi.org/10.1515/pac-2023-1128

Keywords for this article

Allium sativum; antioxidants; minerals; Momordica charantia; Moringa oleifera; phytochemistry; VCCA-2023