Startseite Real-time in-situ 1H NMR of reactions in peptide solution: preaggregation of amyloid-β fragments prior to fibril formation
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Real-time in-situ 1H NMR of reactions in peptide solution: preaggregation of amyloid-β fragments prior to fibril formation

  • Emiko Okamura EMAIL logo und Kenzo Aki
Veröffentlicht/Copyright: 3. August 2020
Pure and Applied Chemistry
Aus der Zeitschrift Pure and Applied Chemistry Band 92 Heft 10

Abstract

In-situ analytical methods are essential for the reliable observation of peptide reactions without perturbation of the system. In this work, a real-time in-situ NMR analysis was performed to gain insight into the initial stage of the aggregation of amyloid-beta (Aβ) 8–25 monomers, S8GY10EVHHQKLVFF20AEDVG25, in solution prior to the fibril formation. NMR chemical shift and intensity changes in combination with the CD spectra revealed no changes in Aβ secondary structure, but the presence of soluble, oligomeric intermediates followed by the appearance of insoluble and non-structured aggregates before β-fibril formation. Molecular views of intermediates and aggregation mechanisms were proposed in comparison with NMR spectral changes in wild-type Aβ 8–25 and its two mutants, A21G and E22G. The mutation of just one amino acid modified the aggregation properties of Aβ 8–25; it slowed or accelerated the fibril formation by controlling the progress of conversion from monomer to aggregate via a soluble, small oligomer.

Keywords: Amyloid-β; biological reaction; ICSC-36; oligomers; preaggregation; real-time NMR; spectroscopy

Article note:

A collection of invited papers based on presentations at the 36th International Conference of Solution Chemistry (ICSC-36), held in Xining, China, 4–8 August 2019.

Corresponding author: Emiko Okamura, Faculty of Pharmaceutical Sciences, Himeji Dokkyo University, 7-2-1, Kamiohno, Himeji 670-8524, Japan, E-mail:

Funding source: Hyogo Science and Technology Association, Japan

Award Identifier / Grant number: 25073

Funding source: Japan Society for the Promotion of Science, Japan

Award Identifier / Grant number: 15K04501

Award Identifier / Grant number: 19K05394

Acknowledgments

The authors also thank Yui Uchihara, Tetsurou Nishida, and Kotoha Kose for sample preparation and data analyses of NMR and CD spectra.

  1. Funding: This work was partly supported by Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science (15K04501, 19K05394) and by Hyogo Science and Technology Association (No. 25073).

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/pac-2019-1201).

Published Online: 2020-08-03
Published in Print: 2020-10-25

© 2020 IUPAC & De Gruyter. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. For more information, please visit: http://creativecommons.org/licenses/by-nc-nd/4.0/

Artikel in diesem Heft

  1. Frontmatter
  2. In this issue
  3. Preface
  4. Selected papers from the 36th International Conference on Solution Chemistry (ICSC-36)
  5. Conference papers
  6. Using computational chemistry to explore experimental solvent parameters – solvent basicity, acidity and polarity/polarizability
  7. Solution chemistry in the surface region of aqueous solutions
  8. Water confined in solutions of biological relevance
  9. Real-time in-situ 1H NMR of reactions in peptide solution: preaggregation of amyloid-β fragments prior to fibril formation
  10. Free energy profile of permeation of Entecavir through Hepatitis B virus capsid studied by molecular dynamics calculation
  11. Dielectric relaxation spectroscopy: an old-but-new technique for the investigation of electrolyte solutions
  12. Excess spectroscopy and its applications in the study of solution chemistry
  13. Structure of aqueous sodium acetate solutions by X-Ray scattering and density functional theory
  14. Desymmetrization in geometry optimization: application to an ab initio study of copper(I) hydration
  15. Interactions between adsorbents and adsorbates in aqueous solutions
  16. Modeling vapor-liquid-liquid-solid equilibrium for acetone-water-salt system
  17. Apparent molar volumes of sodium arsenate aqueous solution from 283.15 K to 363.15 K at ambient pressure: an experimental and thermodynamic modeling study
  18. Extraction of various metal ions by open-chain crown ether bridged diphosphates in supercritical carbon dioxide
  19. Solvation heterogeneity in ionic liquids as demonstrated by photo-chemical reactions
  20. The structure and composition of solid complexes comprising of Nd(III), Ca(II) and D-gluconate isolated from solutions relevant to radioactive waste disposal
  21. Separation of phenols from oils using deep eutectic solvents and ionic liquids
https://doi.org/10.1515/pac-2019-1201
Schlagwörter für diesen Artikel
Amyloid-β; biological reaction; ICSC-36; oligomers; preaggregation; real-time NMR; spectroscopy

Artikel in diesem Heft

  1. Frontmatter
  2. In this issue
  3. Preface
  4. Selected papers from the 36th International Conference on Solution Chemistry (ICSC-36)
  5. Conference papers
  6. Using computational chemistry to explore experimental solvent parameters – solvent basicity, acidity and polarity/polarizability
  7. Solution chemistry in the surface region of aqueous solutions
  8. Water confined in solutions of biological relevance
  9. Real-time in-situ 1H NMR of reactions in peptide solution: preaggregation of amyloid-β fragments prior to fibril formation
  10. Free energy profile of permeation of Entecavir through Hepatitis B virus capsid studied by molecular dynamics calculation
  11. Dielectric relaxation spectroscopy: an old-but-new technique for the investigation of electrolyte solutions
  12. Excess spectroscopy and its applications in the study of solution chemistry
  13. Structure of aqueous sodium acetate solutions by X-Ray scattering and density functional theory
  14. Desymmetrization in geometry optimization: application to an ab initio study of copper(I) hydration
  15. Interactions between adsorbents and adsorbates in aqueous solutions
  16. Modeling vapor-liquid-liquid-solid equilibrium for acetone-water-salt system
  17. Apparent molar volumes of sodium arsenate aqueous solution from 283.15 K to 363.15 K at ambient pressure: an experimental and thermodynamic modeling study
  18. Extraction of various metal ions by open-chain crown ether bridged diphosphates in supercritical carbon dioxide
  19. Solvation heterogeneity in ionic liquids as demonstrated by photo-chemical reactions
  20. The structure and composition of solid complexes comprising of Nd(III), Ca(II) and D-gluconate isolated from solutions relevant to radioactive waste disposal
  21. Separation of phenols from oils using deep eutectic solvents and ionic liquids
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