Amniotic fluid as a source of multipotent cells for clinical use
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Bruce K. Young
,
Michael K. Chan
Abstract
Amniotic fluid cells (AFC) from 2nd trimester amniocentesis have been found to be a source of multipotent stem cells which might overcome the limitations of expansion, histocompatibility, tumorigenesis, and ethical issues associated with using human embryonic cells, umbilical cord, cord blood, bone marrow, and induced pluripotent cells. Previous work by our group and others demonstrated multipotency and the ability to grow well in culture. However, all these studies were done in media containing fetal calf serum. We sought to observe the properties of AFC grown in serum-free media as that would be required for clinical transplantation in humans. Fresh samples were obtained from three patients, and each sample divided into a culture whose cells were not exposed to fetal calf serum, and the other half into a standard culture medium containing fetal calf serum. Doubling time and stem cell marker expression by flow cytometry were assessed. Differentiation to neural, osteoid, and chondrogenic lineages was induced using appropriate media and confirmed by fluorescent microscopy, histology, and immunohistochemistry. There were no statistically significant differences between cells grown serum-free and in standard media in any of these parameters. The data supports the possibility of clinical use of AFC in stem cell transplantation.
Acknowledgments
This work was supported by a grant from the William and Linda Haugland Foundation.
References
[1] Shahrokhi S, Menaa F, Alimoghaddam K, McGuckin C, Ebtekar M. Insights and hopes in umbilical cord blood stem cell transplantations. J Biomed Biotechnol. 2012;57:28–31.10.1155/2012/572821Suche in Google Scholar PubMed PubMed Central
[2] Da Sacco S, De Filippo RE, Perin L. Amniotic fluid as a source of pluripotent and multipotent stem cells for organ regeneration. Curr Opin Organ Transplant. 2010;16:101–5.10.1097/MOT.0b013e3283424f6eSuche in Google Scholar PubMed
[3] De Coppi P, Bartsch G, Jr., Siddiqui MM, Xu T, Santos CC, Perin L, et al. Isolation of amniotic stem cell lines with potential for therapy. Nat Biotechnology. 2007;25:100–6.10.1038/nbt1274Suche in Google Scholar PubMed
[4] Orciani M, Emanuelli M, Martino C, Pugnaloni A, Tranquilli AL, Di Primio R. Potential role of culture mediums for successful isolation and neuronal differentiation of amniotic fluid stem cells. Int J Immunopathol Pharmacol. 2008;21:595–602.10.1177/039463200802100312Suche in Google Scholar PubMed
[5] Kolambkar YM, Peister A, Soker S, Atala A, Guldberg RE. Chondrogenic differentiation of amniotic fluid-derived stem cells. J Mol Histol. 2007;38:405–13.10.1007/s10735-007-9118-1Suche in Google Scholar PubMed
[6] Chen Z, Chan MK, Strelchenko N, Wang F, Liu L, Perle MA, et al. Heterogeneity of stem cells in human amniotic fluid. J Regen Med. 2014;3:1.Suche in Google Scholar
[7] Zheng YB, Gao ZL, Xie C, Zhu HP, Peng L, Chen JH, et al. Characterization and hepatogenic differentiation of mesenchymal stem cells from human amniotic fluid and human bone marrow: a comparative study. Cell Biol Int. 2008;32:1439–48.10.1016/j.cellbi.2008.08.015Suche in Google Scholar PubMed
[8] Kim SW, Zhang HZ, Kim CE, An HS, Kim JM, Kim MH. Amniotic mesenchymal stem cells have robust angiogenic properties and are effective in treating hindlimb ischaemia. Cardiovasc Res. 2012;93:525–34.10.1093/cvr/cvr328Suche in Google Scholar PubMed
[9] Kim J, Lee Y, Kim H, Hwang KJ, Kwon HC, Kim SK, et al. Human amniotic fluid-derived stem cells have characteristics of multipotent stem cells. Cell Prolif. 2007;40:75–90.10.1111/j.1365-2184.2007.00414.xSuche in Google Scholar PubMed PubMed Central
[10] Zhang P, Baxter J, Vinod K, Tulenko TN, Di Muzio PJ. Endothelial differentiation of amniotic fluid-derived stem cells: synergism of biochemical and shear force stimuli. Stem Cells Dev. 2009;18:1299–308.10.1089/scd.2008.0331Suche in Google Scholar PubMed PubMed Central
[11] Klemmt PA, Vafaizadeh V, Groner B. The potential of amniotic fluid stem cells for cellular therapy and tissue engineering. Expert Opin Bio Ther. 2011;11:1297–314.10.1517/14712598.2011.587800Suche in Google Scholar PubMed
[12] Rosner M, Mikula M, Preitschopf A, Feichtinger M, Schipany K, Hengstschläger M. Neurogenic differentiation of amniotic fluid stem cells. Amino Acids. 2012;42:1591–6.10.1007/s00726-011-0929-8Suche in Google Scholar PubMed
[13] Zia S, Toelen J, daCunha MM, DeKonick P, deCoppi P, Deprest J. Routine clonal expansion of mesenchymal stem cells derived from amniotic fluid for perinatal applications. Prenat Diagn. 2013;33:1–8.10.1002/pd.4162Suche in Google Scholar PubMed
The authors stated that there are no conflicts of interest regarding the publication of this article.
©2016 by De Gruyter
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- Frontmatter
- Editorial
- The emergence of obstetric medicine
- Mini review
- Folate status and health: challenges and opportunities
- Original articles - Obstetrics
- Increased levels of serum clusterin is associated with intrauterine growth restriction and adverse pregnancy outcomes in preeclampsia
- The utility of clinical findings to predict laboratory values in hypertensive disorders of pregnancy
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- Are there anamnestic risk factors for iron deficiency in pregnancy? Results from a feasibility study
- Is there any relationship between serum sirtuin-1 level and neutrophil-lymphocyte ratio in hyperemesis gravidarum?
- Pregnancy outcomes among renal transplant recipients and patients with end-stage renal disease on dialysis
- Are pregnant women safer in motor vehicle accidents?
- Amniotic fluid as a source of multipotent cells for clinical use
- Original articles - Newborn
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- Effects of preeclampsia on the amplitude integrated electroencephalography activity in preterm infants
- Short communication
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