Plasma but not serum brain-derived neurotrophic factor concentration is decreased by oral glucose tolerance test-induced hyperglycemia in children

Shunsuke Araki; Yukiyo Yamamoto; Reiko Saito; Aoi Kawakita; Mami Eguchi; Motohide Goto; Kazuyasu Kubo; Rinko Kawagoe; Yasusada Kawada; Koichi Kusuhara

doi:10.1515/jpem-2016-0344

Article

Plasma but not serum brain-derived neurotrophic factor concentration is decreased by oral glucose tolerance test-induced hyperglycemia in children

Shunsuke Araki , Yukiyo Yamamoto , Reiko Saito , Aoi Kawakita , Mami Eguchi , Motohide Goto , Kazuyasu Kubo , Rinko Kawagoe , Yasusada Kawada and Koichi Kusuhara

Published/Copyright: March 22, 2017

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From the journal Journal of Pediatric Endocrinology and Metabolism Volume 30 Issue 5

Abstract

Background:

Little is known regarding the relationships among circulating brain-derived neurotrophic factor (BDNF) levels and glucose or insulin in children and adolescents. The objective of this study was to investigate whether circulating BDNF levels would change during the oral glucose tolerance test (OGTT).

Methods:

We performed the OGTT and measured the serial changes in BDNF levels in both plasma and serum.

Results:

There were 22 subjects in the normal type (N) group and 20 in the borderline/diabetic type (B/D) group, defined by the results of the OGTT. Serum levels of BDNF were almost five times higher and plasma levels gradually decreased during the OGTT, whereas serum levels showed no significant change. The reduction of plasma BDNF level changes from baseline to 120 min were significantly different between the N and B/D groups (36.3% vs. 20.8%, p=0.023).

Conclusions:

Our results showed that plasma levels of BDNF are more sensitive to acute changes in glucose or insulin levels than serum.

Keywords: brain-derived neurotrophic factor; child and adolescents; glucose tolerance test; insulin; platelets

Corresponding author: Shunsuke Araki, MD, PhD, Department of Pediatrics, School of Medicine, University of Occupational and Environmental Health, 1-1 Iseigaoka, Yahatanishi-ku, Kitakyushu 807-8555, Japan, Phone: +81 (93) 691-7254, Fax: +81 (93) 691-9338

Acknowledgments

The authors thank Miss Yuki Ohga for her technical assistance.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work was supported by a Grant-in-Aid for Scientific Research #25860893 (to S.A.) and #21591340 (to Y.Y.) from the Ministry of Education, Culture, Sports, Science and Technology, Japan.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

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Received: 2016-8-31

Accepted: 2017-2-2

Published Online: 2017-3-22

Published in Print: 2017-5-1

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https://doi.org/10.1515/jpem-2016-0344

Keywords for this article

brain-derived neurotrophic factor; child and adolescents; glucose tolerance test; insulin; platelets