Beneficial consequences of probiotic on mitochondrial hippocampus in Alzheimer’s disease

Doha M. Beltagy; Nagat F. Nawar; Tarek M. Mohamed; Ehab Tousson; Mai M. El-Keey

doi:10.1515/jcim-2020-0156

Artikel

Beneficial consequences of probiotic on mitochondrial hippocampus in Alzheimer’s disease

Doha M. Beltagy , Nagat F. Nawar , Tarek M. Mohamed , Ehab Tousson und Mai M. El-Keey

Veröffentlicht/Copyright: 30. März 2021

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Aus der Zeitschrift Journal of Complementary and Integrative Medicine Band 18 Heft 4

Abstract

Background

Alzheimer’s (AD) is one of the most common neurodegenerative diseases, causing dementia and brain cells death.

Objectives

This study aimed to assess the ameliorating effect of Acidophilus probiotic against AD induced in rats by d-galactose and AlCl₃ injection via evaluating mitochondrial parameter changes in hippocampus.

Methods

This study was carried out on rats were classified into five groups; G1 (control group), G2 (probiotic group), G3 (AD group), G4 (co-treated group) and G5 (post-treated group). By the end of the experiment, some different neurotransmitters, oxidative stress biomarkers, zinc, blood glucose, Na⁺K⁻ATPase subunit alpha 1 (ATP1A1), and gene expression of mitochondrial membrane potential (MMP) were measured.

Results

Significant changes in neurotransmitters, antioxidants levels and decreased ATP1A1 activity and gene expression of MMP in the hippocampus in G3 were detected if compared to control. Best improvement in G5 than G4 group was observed. These results were confirmed by histological and immunohistochemical studies in hippocampus.

Conclusions

Acidophilus probiotic was able to alleviate learning and memory associated injuries in AD by reducing mitochondrial dysfunction induced by d-galactose and AlCl₃. This may be associated with its antioxidant properties.

Keywords: acidophilus probiotic; Alzheimer’s; antioxidants; d-galactose; neurotransmitters

Corresponding author: Doha M. Beltagy, Biochemistry Division, Chemistry Department, Faculty of Science, Damanhour University, Damanhour, Egypt, Phone: +20 1224778799, E-mail: dohabel4@yahoo.com

Research funding: None declared.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Competing interests: The authors declare that they have no conflict of interest.
Ethical approval: This experiment was carried out in accordance with the ethical protocol of Faculty of Science, Tanta University with Code No. REC-SCI-TU-27.

References

1. Bhushan, I, Kour, M, Kour, G, Gupta, S, Sharma, S, Yadav, A. Alzheimer’s disease: causes & treatment – a review. Ann Biotechnol 2018;1:1002. https://doi.org/10.33582/2637-4927/1002.Suche in Google Scholar

2. Armstrong, RA. What causes Alzheimer’s disease? Folia Neuropathol 2013;51:169–88. https://doi.org/10.5114/fn.2013.37702.Suche in Google Scholar PubMed

3. Tönnies, E, Trushina, E. Oxidative stress, synaptic dysfunction, and Alzheimer’s disease. J Alzheim Dis 2017;57:1105–21. https://doi.org/10.3233/jad-161088.Suche in Google Scholar

4. Islam, MS, Choi, H. Antidiabetic effect of Korean traditional Baechu (Chinese cabbage) Kimchi in a type 2 diabetes model of rats. J Med Food 2009;12:292–7. https://doi.org/10.1089/jmf.2008.0181.Suche in Google Scholar PubMed

5. Bernardeau, M, Vernoux, JP, Henri-Dubernet, S, Guéguen, M. Safety assessment of dairy microorganisms, the Lactobacillus genus. Int J Food Microbiol 2008;126:278–85. https://doi.org/10.1016/j.ijfoodmicro.2007.08.015.Suche in Google Scholar PubMed

6. Masood, MI, Qadir, MI, Shirazi, JH, Khan, IU. Beneficial effects of lactic acid bacteria on human beings. Crit Rev Microbiol 2011;37:91–8. https://doi.org/10.3109/1040841x.2010.536522.Suche in Google Scholar

7. Vijayakumar, J, Aravindan, R, Viruthagiri, T. Recent trends in the production, purification and application of lactic acid. Chem Biochem Eng 2008;22:245–64.Suche in Google Scholar

8. Peng, X, Meng, J, Chi, T, Liu, P, Man, C, Liu, S, et al.. Lactobacillus plantarum NDC 75017 alleviates the learning and memory ability in aging rats by reducing mitochondrial dysfunction. Exp Ther Med 2014;8:1841–6. https://doi.org/10.3892/etm.2014.2000.Suche in Google Scholar PubMed PubMed Central

9. Mallikarjuna, N, Praveen, K, Yellamma, K. Role of Lactobacillus plantarum MTCC1325 in membrane-bound transport ATPases system in Alzheimer’s disease-induced rat brain. BioImpacts 2016;6:203–9. https://doi.org/10.15171/bi.2016.27.Suche in Google Scholar PubMed PubMed Central

10. Lakshmi, BV, Sudhakar, M, Prakash, SK. Protective effect of selenium against aluminum chloride-induced Alzheimer’s disease: behavioral and biochemical alterations in rats. Biol Trace Elem Res 2015;165:67–74. https://doi.org/10.1007/s12011-015-0229-3.Suche in Google Scholar PubMed

11. Serchov, T, van Calker, D, Biber, K. Light/dark transition test to assess anxiety-like behavior in mice. Bio-protocol 2016;6:1–4. https://doi.org/10.21769/bioprotoc.1957.Suche in Google Scholar

12. Hayakawa, R. Determination of zinc. Jpn J Toxic Environ Health 1961;8:14–8. https://doi.org/10.1248/jhs1956.8.2_14.Suche in Google Scholar

13. Montogomery, HA, Dymock, JF. The determination of nitrite in water. Analyst 1961;84:414–6.Suche in Google Scholar

14. Trinder, P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann din Biochem 1969;6:24–7. https://doi.org/10.1177/000456326900600108.Suche in Google Scholar

15. Ohkawa, H, Ohishi, W, Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8. https://doi.org/10.1016/0003-2697(79)90738-3.Suche in Google Scholar

16. Aebi, H. Catalase in vitro. Methods in enzymology, vol. 105. Elsevier; 1984:121–6pp.10.1016/S0076-6879(84)05016-3Suche in Google Scholar

17. Nishikimi, M, Roa, NA, Yogi, K. The occurrence of supeoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biophys Res Commun 1972;46:849–54. https://doi.org/10.1016/s0006-291x(72)80218-3.Suche in Google Scholar

18. Paglia, DE, Valentine, WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967;70:158–69.Suche in Google Scholar

19. Lu, T, Xu, Y, Mericle, MT, Mellgren, RL. Participation of the conventional calpains in apoptosis. Biochim Biophys Acta 2002;1590:16–26. https://doi.org/10.1016/s0167-4889(02)00193-3.Suche in Google Scholar

20. Monis, PT, Giglio, S. Nucleic acid amplification-based techniques for pathogen detection and identification. Infect Genet Evol 2006;6:2–12. https://doi.org/10.1016/j.meegid.2005.08.004.Suche in Google Scholar PubMed PubMed Central

21. Tousson, E. Histopathological alterations after a growth promoter boldenone injection in rabbits. TIH 2016;32:299–305. https://doi.org/10.1177/0748233713500821.Suche in Google Scholar PubMed

22. Tousson, E, Ali, EM, Ibrahim, W, Ashraf, RM. Histopathological and immunohistochemical alterations in rat heart after thyroidectomy and the role of Hemin and ketoconazole in treatment. Biomed Pharmacother 2012;66:627–32. https://doi.org/10.1016/j.biopha.2012.08.009.Suche in Google Scholar PubMed

23. Jung, IH, Jung, MA, Kim, EJ, Hem, MJ, Kim, DH. Lactobacillus pentosus var. plantarum C29 protects scopolamine induced memory deficit in mice. J Appl Microbiol 2012;113:1498–506. https://doi.org/10.1111/j.1365-2672.2012.05437.x.Suche in Google Scholar PubMed

24. Al-Okbi, SY, Abdel-Razek, AG, Mohammed, SE, Ottai, MES. Roselle seed as a potential new source of healthy edible oil. J Biol Sci 2017;17:267–77. https://doi.org/10.3923/jbs.2017.267.277.Suche in Google Scholar

25. Burns, CM, Chen, K, Kaszniak, AW, Lee, W, Alexander, GE, Bandy, D, et al.. Higher serum glucose levels are associated with cerebral hypo metabolism in Alzheimer regions. Neurology 2013;80:1557–64. https://doi.org/10.1212/wnl.0b013e31828f17de.Suche in Google Scholar

26. Lane, RM, Potkin, SG, Enz, A. Targeting acetyl cholinesterase and butyryl cholinesterase in dementia. Int J Neuropsychopharmacol 2006;9:101–24. https://doi.org/10.1017/S1461145705005833.Suche in Google Scholar PubMed

27. Li, SM, Mo, MS, Xu, PY. Progress in mechanisms of acetylcholinesterase inhibitors and memantine for treatment of Alzheimer’s disease. Neuroimmunol Neuroinflammation 2015;2:274–80.10.4103/2347-8659.167305Suche in Google Scholar

28. Nimgampalle, M, Kuna, Y. Anti-Alzheimer properties of probiotic, Lactobacillus plantarum MTCC 1325 in Alzheimer’s disease induced albino rats. J Clin Diagn Res 2017;11:KC01–5. https://doi.org/10.7860/JCDR/2017/26106.10428.Suche in Google Scholar PubMed PubMed Central

29. Mishra, P, Maurya, RK, Dwivedi, D. Effect of dopamine on Alzheimer and autism and determination of best model organism for both. IJSRP 2013;3:2250–3153.Suche in Google Scholar

30. Tsai, SJ, Lin, CY, Mong, MC, Ho, MW, Yin, MC. nS-Ethyl cysteine and S-propyl cysteine alleviate beta-amyloid induced cytotoxicity in nerve growth factor differentiated PC12 cells. J Agric Food Chem 2010;58:7104–8. https://doi.org/10.1021/jf1009318.Suche in Google Scholar PubMed

31. Rai, AK, Bhaskar, N, Baskaran, V. Effect of feeding lipids recovered from fish processing waste by lactic acid fermentation and enzymatic hydrolysis on antioxidant and membrane bound enzymes in rats. J Food Sci Technol 2015;52:3701–10. https://doi.org/10.1007/s13197-014-1442-3.Suche in Google Scholar PubMed PubMed Central

32. Balez, R, Ooi, L. Getting to no Alzheimer’s disease: neuroprotection versus neurotoxicity mediated by nitric oxide. Oxid Med Cell Longev 2015;2016:1–8.10.1155/2016/3806157Suche in Google Scholar PubMed PubMed Central

33. Venkateshappa, C, Harish, G, Mahadevan, A, Bharath, MS, Shankar, S. Elevated oxidative stress and decreased antioxidant function in the human hippocampus and frontal cortex with increasing age. Implications for neurodegeneration in Alzheimer’s disease. Neurochem Res 2012;37:1601–14. https://doi.org/10.1007/s11064-012-0755-8.Suche in Google Scholar PubMed

34. Iriondo-DeHond, A, Martorell, P, Genoves, S, Ramon, D, Stamatakis, K, Fresno, M, et al.. Coffee silver skin extract protects against accelerated aging caused by oxidative agents. Molecules 2016;21:721. https://doi.org/10.3390/molecules21060721.Suche in Google Scholar PubMed PubMed Central

35. Watt, NT, Whitehouse, IJ, Hooper, NM. Role of zinc in Alzheimer’s disease. Int J Alzheim Dis 2010;2011:971021.10.4061/2011/971021Suche in Google Scholar

36. Rulon, LL, Robertson, JD, Lovell, MA, Deibel, MA, Ehmann, WD, Markesbery, WR. Serum zinc levels and Alzheimer’s disease. Biol Trace Elem Res 2000;75:79–85. https://doi.org/10.1385/bter:75:1-3:79.10.1385/BTER:75:1-3:79Suche in Google Scholar

37. Perry, SW, Norman, JP, Barbieri, J, Brown, EB, Gelbard, HA. Mitochondrial membrane potential probes and the proton gradient: a practical usage guide. Biotechniques 2018;50:98–114.10.2144/000113610Suche in Google Scholar PubMed PubMed Central

38. Liu, YJ, Mclntyre, RL, Janssens, GE, Houtkooper, RH. Mitochondrial fission and fusion: a dynamic role in aging and potential targer for age-related disease. Mech Ageing Dev 2020;186:111212. https://doi.org/10.1016/j.mad.2020.111212.Suche in Google Scholar PubMed

Received: 2020-05-15

Accepted: 2020-08-23

Published Online: 2021-03-30

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Schlagwörter für diesen Artikel

acidophilus probiotic; Alzheimer’s; antioxidants; d-galactose; neurotransmitters