Evidence of attenuation of intestinal ischemia–reperfusion injury following pre-treatment with methanolic extracts from Chromolena odorata in rats
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Joseph Fadeyemi Akinrinmade
, Atinuke Odejobi
Abstract
Background: Chromolena odorata is a tropical species of flowering shrub in the family Asteraceae, leaves of it have been reported to be widely used as herbal remedy for the treatment of various ailments. It is particularly reported to be useful in the healing of wounds.
Methods: We investigated the possibility of amelioration of intestinal ischemia–reperfusion (IR) injury in rats treated with methanolic extract of C. odorata (MECO). Wistar albino rats were divided randomly into five groups of six animals each as control, IR-treated, IR+200 mg/kg MECO, IR+400 mg/kg MECO, and IR+200 mg/kg vitamin C. Pre-treatment with MECO or vitamin C was for 7 days.
Results: The contents of hydrogen peroxide (H2O2) and malondialdehyde (MDA) were significantly reduced by MECO and vitamin C, while there were significant enhancements of the activities of superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT), as well as the content of reduced glutathione (GSH) in pre-treated rats compared to IR-treated rats. Glutathione S-transferase (GST) activity was not significantly affected in all the groups. Histopathological examination of small intestinal mucosa revealed significant attenuation of intestinal pathology in animals pre-treated with MECO, while IR injury produced severe villi erosion, necrosis, and inflammatory cell infiltrations.
Conclusions: The present study highlights the antioxidant activities of MECO and its ability to inhibit inflammatory cell infiltration as mechanisms involved in its protection against IR injury in the intestine of rats, an effect that was largely comparable to that of vitamin C.
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©2015 by De Gruyter
Artikel in diesem Heft
- Frontmatter
- Review
- An emphasis on molecular mechanisms of anti-inflammatory effects and glucocorticoid resistance
- Preclinical Studies
- Endothelium nitric oxide-independent vasorelaxant effects of the aqueous extract from Stephania abyssinica on the isolated rat thoracic aorta
- Evidence of attenuation of intestinal ischemia–reperfusion injury following pre-treatment with methanolic extracts from Chromolena odorata in rats
- Comparative effects of Orchis anatolica vs. the red Korean Panax ginseng treatments on testicular structure and function of adult male mice
- The Miaoyao Fanggan Sachets regulate humoral immunity and cellular immunity in mice
- Toxicological evaluation of the aqueous extract of Acalypha wilkesiana in Wistar albino rats
- Clinical Studies
- Tolerability of the combination of ginger (Zingiber officinalis), gentian (Gentiana lutea) and turmeric (Curcuma longa) in patients with cancer-associated anorexia
- Effect of rhubarb (Rheum emodi) in primary dysmenorrhoea: a single-blind randomized controlled trial
- Integrating traditional medical practice with primary healthcare system in Eritrea
- Factors affecting the use of complementary and alternative medicine among Japanese university students
- Short Communication
- Complementary and alternative medicine in diabetes (CALMIND) – a prospective study
- Online Only: Congress Abstracts
- 2014 IN-CAM Research Symposium: The Next Wave of Complementary and Integrative Medicine and Health Care Research
Artikel in diesem Heft
- Frontmatter
- Review
- An emphasis on molecular mechanisms of anti-inflammatory effects and glucocorticoid resistance
- Preclinical Studies
- Endothelium nitric oxide-independent vasorelaxant effects of the aqueous extract from Stephania abyssinica on the isolated rat thoracic aorta
- Evidence of attenuation of intestinal ischemia–reperfusion injury following pre-treatment with methanolic extracts from Chromolena odorata in rats
- Comparative effects of Orchis anatolica vs. the red Korean Panax ginseng treatments on testicular structure and function of adult male mice
- The Miaoyao Fanggan Sachets regulate humoral immunity and cellular immunity in mice
- Toxicological evaluation of the aqueous extract of Acalypha wilkesiana in Wistar albino rats
- Clinical Studies
- Tolerability of the combination of ginger (Zingiber officinalis), gentian (Gentiana lutea) and turmeric (Curcuma longa) in patients with cancer-associated anorexia
- Effect of rhubarb (Rheum emodi) in primary dysmenorrhoea: a single-blind randomized controlled trial
- Integrating traditional medical practice with primary healthcare system in Eritrea
- Factors affecting the use of complementary and alternative medicine among Japanese university students
- Short Communication
- Complementary and alternative medicine in diabetes (CALMIND) – a prospective study
- Online Only: Congress Abstracts
- 2014 IN-CAM Research Symposium: The Next Wave of Complementary and Integrative Medicine and Health Care Research