Structure elucidation and inhibitory effects on human platelet aggregation of chlorogenic acid from Wrightia tinctoria
-
Ripal P. Amin#
und S. William Zito
Abstract
Background: Interest in natural compounds as sources of potentially new treatment options is growing rapidly. Preliminary screening of many different plant extracts showed that Wrightia tinctoria acts as a potent human platelet aggregation inhibitor. The aim of the present study was to isolate and characterize the active compound responsible for potent inhibition of human platelet aggregation in vitro.
Methods: A 70% ethanolic extract derived from W. tinctoria seeds was fractionated with chloroform followed by ethyl acetate. The ethyl acetate fraction was further fractionated and purified through a series of three successive column chromatographic separations using silica gel, Sephadex LH‐20, and C‐18 columns. Liquid chromatography coupled to negative electrospray ionization tandem mass spectrometry (LC‐MS/MS) and nuclear magnetic resonance (NMR) studies were performed in the structure determination of the active phenolic compound present in the ethyl acetate fraction of W. tinctoria seeds.
Results: A phenolic compound has been isolated and identified as chlorogenic acid by LC‐MS/MS and NMR studies. Chlorogenic acid showed concentration-dependent inhibitory effect on collagen-induced platelet aggregation in vitro with an IC50 of 0.2363 μg/μl.
Conclusion: The present data suggest that chlorogenic acid can be developed as potential antiplatelet agent in the treatment of cardiovascular diseases in diabetes mellitus.
Acknowledgements
The authors thank Mr. Rahulkumar A. Parakhia (College of Pharmacy and Health Professions, St John’s University) for his critical reading of manuscript.
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©2013 by Walter de Gruyter Berlin / Boston
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Artikel in diesem Heft
- Masthead
- Masthead
- Phytochemistry & Product Quality
- Chemical and pharmacological standardization of Ashwagandhadi lehyam: an ayurvedic formulation
- Phytochemical screening and toxicity studies on the methanol extract of the seeds of moringa oleifera
- First report of fingerprinting dried herbal products using a subtractive diversity array
- Preclinical Studies
- Antidiabetic and antihypertensive effect of Virgin Argan Oil in model of neonatal streptozotocin-induced diabetic and l-nitroarginine methylester (l-NAME) hypertensive rats
- Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice
- Aloe vera gel protects liver from oxidative stress-induced damage in experimental rat model
- Hepatoprotective activity of Calotropis gigantea flowers against carbon-tetrachloride-induced liver damage in mice
- In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae)
- Liposomal-formulated curcumin [Lipocurc™] targeting HDAC (Histone Deacetylase) prevents apoptosis and improves motor deficits in Park 7 (DJ-1)-knockout rat model of Parkinson’s disease: implications for epigenetics-based nanotechnology-driven drug platform
- Antimalarial potential of China 30 and Chelidonium 30 in combination therapy against lethal rodent malaria parasite: Plasmodium berghei
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- Cytotoxic effects of crocin on MOLT-4 human leukemia cells
- Synergistic cytotoxicity of red beetroot (Beta vulgaris L.) extract with doxorubicin in human pancreatic, breast and prostate cancer cell lines
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