First report of fingerprinting dried herbal products using a subtractive diversity array
-
Linhai Niu
,
Hans Wohlmuth
Abstract
Introduction Sequence-independent microarrays have never been used to identify and authenticate dried herbal plants. Methods: We report successful fingerprinting of seven species: Leonurus sibiricus, Astragalus membranaceus, Coix lachryma-jobi, Magnolia biondii, Abutilon theophrasti, Physalis alkekengi, and Salvia miltiorrhiza from dried tissues using a sequence-independent microarray, “Subtracted Diversity Array”.
Results: Herbal plants could be identified from tissues as they were sold at the clinic. Hierarchical cluster of these species generated using SPSS v.15.0 confirmed to their predicted taxonomical relationships as specified in the Angiosperm Phylogeny Group II classification system. A polymorphism rate of 40.7% was achieved from the 376 spots used for fingerprinting. Functional characterization of polymorphic features by sequencing revealed 27.1% of those were retroelements or genes.
Conclusions: This technique provides a new way to produce markers for authenticating dried herbal samples.
References
1. Ralt D. Intercellular communication, NO and the biology of Chinese medicine. Cell Commun Signal 2005;3:8.10.1186/1478-811X-3-8Search in Google Scholar
2. Yeh CC, Lin CC, Wang SD, Hung CM, Yeh MH, Liu CJ, et al. Protective and immunomodulatory effect of Gingyo-san in a murine model of acute lung inflammation. J Ethnopharmacol 2007;111:418–26.10.1016/j.jep.2006.12.014Search in Google Scholar
3. An W, Yang J. Protective effects of Ping-Lv-Mixture (PLM), a medicinal formula on arrhythmias induced by myocardial ischemia-reperfusion. J Ethnopharmacol 2006;108:90–5.10.1016/j.jep.2006.04.009Search in Google Scholar
4. Scheid V. The globalisation of Chinese medicine. Lancet 2000;354:10.10.1016/S0140-6736(99)90353-7Search in Google Scholar
5. Patwardhan B, Warude D, Pushpangadan P, Bhatt N. Ayurveda and traditional Chinese medicine: a comparative overview. Evid Based Complement Alternat Med 2005;2:465–73.10.1093/ecam/neh140Search in Google Scholar PubMed PubMed Central
6. Zhu YP. Toxicity of the Chinese herb mu tong (Aristolochia manshuriensis). What history tells us. Adverse Drug React Toxicol Rev 2002;21:171–7.10.1007/BF03256194Search in Google Scholar PubMed
7. Zhang YB, Wang J, Wang ZT, But PP, Shaw PC. DNA microarray for identification of the herb of dendrobium species from Chinese medicinal formulations. Planta Med 2003;69:1172–4.10.1055/s-2003-818015Search in Google Scholar PubMed
8. Zhang YB, Shaw PC, Sze CW, Wang ZT, Tong Y. Molecular authentication of Chinese herbal materials. J Food Drug Anal 2007;15:1–9.10.38212/2224-6614.2449Search in Google Scholar
9. Um JY, Chung HS, Kim MS, Na HJ, Kwon HJ, Kim JJ, et al. Molecular authentication of Panax ginseng species by RAPD analysis and PCR-RFLP. Biol Pharm Bull 2001;24:872–5.10.1248/bpb.24.872Search in Google Scholar PubMed
10. Dangi RS, Lagu MD, Choudhary LB, Ranjekar PK, Gupta VS. Assessment of genetic diversity in Trigonella foenum-graecum and Trigonella caerulea using ISSR and RAPD markers. BMC Plant Biol 2004;4:13.10.1186/1471-2229-4-13Search in Google Scholar PubMed PubMed Central
11. Ha WY, Shaw PC, Liu J, Yau FC, Wang J. Authentication of Panax ginseng and Panax quinquefolius using amplified fragment length polymorphism (AFLP) and directed amplification of minisatellite region DNA (DAMD). J Agric Food Chem 2002;50:1871–5.10.1021/jf011365lSearch in Google Scholar PubMed
12. Xu H, Wang Z, Ding X, Zhou K, Xu L. Differentiation of Dendrobium species used as “Huangcao Shihu” by rDNA ITS sequence analysis. Planta Med 2006;72:89–92.10.1055/s-2005-916228Search in Google Scholar PubMed
13. Li TX, Wang JK, Bai YF, Lu ZH. Diversity Suppression-Subtractive hybridization array for profiling genomic DNA polymorphisms. J Integr Plant Biol 2006;48:460–7.10.1111/j.1744-7909.2006.00249.xSearch in Google Scholar
14. Jaccoud D, Peng KM, Feinstein D, Kilian A. Diversity array: a solid state technology for sequence information independent genotyping. Nucleic Acid Res 2001;29:e25.10.1093/nar/29.4.e25Search in Google Scholar PubMed PubMed Central
15. Carles M, Cheung MK, Moganti S, Dong TT, Tsim KW, Ip NY et al. A DNA microarray for the authentication of toxic traditional Chinese medicinal plants. Planta Med 2005;71:580–4.10.1055/s-2005-864166Search in Google Scholar PubMed
16. Sze SC, Zhang KY, Shaw PC, But PP, Ng TB, Tong YA. DNA microarray for differentiation of (Fengdou Shihu) by its 5S ribosomal DNA intergenic spacer region. Biotechnol Appl Biochem 2008;49:149–54.10.1042/BA20070059Search in Google Scholar PubMed
17. Li TX, Wang JK, Bai YF, Sun XD, Lu ZH. A novel method for screening species-specific gDNA probes for species identification. Nucleic Acids Res 2004;32:e45.10.1093/nar/gnh041Search in Google Scholar PubMed PubMed Central
18. Lezar S, Myburg AA, Berger DK, Wingfield MJ. Development and assessment of microarray-based DNA fingerprinting in Eucalyptus grandis. Theor Appl Genet 2004;109:1329–36.10.1007/s00122-004-1759-9Search in Google Scholar PubMed
19. Jayasinghe R, Kong S, Coram TE, Kaganovitch J, Xue CC, Li CG, et al. Construction and validation of an innovative microarray for novel application of efficient and high-throughput genotyping of Angiosperms. Plant Biotechnol J 2007;5:282–9.10.1111/j.1467-7652.2007.00240.xSearch in Google Scholar PubMed
20. Jayasinghe R, Hai NL, Coram TE, Kong S, Kaganovitch J, Xue CC et al. Effectiveness of an innovative prototype subtracted diversity array (SDA) for fingerprinting plant species of medicinal importance. Planta Med 2009;75:1180–5.10.1055/s-0029-1185484Search in Google Scholar PubMed
21. The Angiosperm Phylogeny Group. An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II. Bot J Linn Soc 2003;141:399–436.10.1046/j.1095-8339.2003.t01-1-00158.xSearch in Google Scholar
22. Wenzl P, Carling J, Kudrna D, Jaccoud D, Huttner E, Klenhofs A. Diversity Array Technology (DArT) for whole-genome profiling of barley. Proc Natl Acad Sci 2004;101:9915–20.10.1073/pnas.0401076101Search in Google Scholar PubMed PubMed Central
23. Yang S, Pang W, Ash G, Harper J, Carling J, Wenzl P, et al. Low level of genetic diversity in cultivated Pigeonpea compared to its wild relatives is revealed by diversity arrays technology. Theor Appl Genet 2006;113:585–95.10.1007/s00122-006-0317-zSearch in Google Scholar PubMed
24. Nouzová M, Neumann P, Navrátilová A, Galbraith DW, Macas J. Microarray-based survey of repetitive genomic sequences in Vicia spp. Plant Mol Biol 2001;45:229–244.10.1023/A:1006408119740Search in Google Scholar
25. Heller-Uszynska K, Uszynski G, Huttner E, Evers M, Carlig J, Caig V, et al. Diversity arrays technology effectively reveals DNA polymorphism in a large and complex genome of sugarcane. Mol Breed 2010. DOI: 10.1007/s11032–010–9460-y.10.1007/s11032-010-9460-ySearch in Google Scholar
26. Jayashree B, Punna R, Prasad P, Bantte K, Hash CT, Chandra S, et al. A database of simple sequence repeats from cereal and legume expressed sequence tags mined in silico: survey and evaluation. In Silico Biol 2006;6:607–20.Search in Google Scholar
©2013 by Walter de Gruyter Berlin / Boston
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- Chemical and pharmacological standardization of Ashwagandhadi lehyam: an ayurvedic formulation
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- First report of fingerprinting dried herbal products using a subtractive diversity array
- Preclinical Studies
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- Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice
- Aloe vera gel protects liver from oxidative stress-induced damage in experimental rat model
- Hepatoprotective activity of Calotropis gigantea flowers against carbon-tetrachloride-induced liver damage in mice
- In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae)
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- Cytotoxic effects of crocin on MOLT-4 human leukemia cells
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- Iatrogenic venous thrombosis secondary to supplemental medicine toxicity
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