Chemical and pharmacological standardization of Ashwagandhadi lehyam: an ayurvedic formulation
-
Arun Rasheed
,
K.V. Satyanarayana
Abstract
Standardization is an important measurement for ensuring the quality control of herbal drugs. It has become essential to develop reliable, specific and sensitive quality control methods. One of the prime ingredients of Ashwagandhadi lehyam is Withania somnifera L. Dunal (Solanaceae) (ashwagandha). In the present study, Ashwagandhadi lehyam was formulated and the quality assessment of the formulation was based on phytochemical screening and physico-chemical evaluation. Ashwagandhadi lehyam was formulated according to a working formula and subjected to phytochemical screening by FTIR analysis and HPTLC fingerprinting, heavy metal determination by AAS, determination of alcohol content, tested for Escherichia coli, Staphylococcus aureus, aerobic bacteria, yeasts and mould, oral toxicity studies and anti-epileptic activity by MES method. The physico-chemical studies showed total ash content as 6.45%, extractive values and some trace elements such as lead, mercury, cadmium and arsenic with 3.2, 0.05, 0.18 and 0.48 ppm, respectively. FTIR and HPTLC studies revealed the presence of functional groups of withanolides in Ashwagandhadi lehyam, resulting in its chemical standardization. The formulation exhibited less epileptic seizures in various phases when compared with that of standard phenytoin and found to possess better anti-inflammatory activity, thus making it biologically standardized. The physico-chemical and pharmacological analysis to standardize Ashwagandhadi lehyam confirmed its use as a safe anti-inflammatory agent and for various seizure disorders.
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©2013 by Walter de Gruyter Berlin / Boston
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Artikel in diesem Heft
- Masthead
- Masthead
- Phytochemistry & Product Quality
- Chemical and pharmacological standardization of Ashwagandhadi lehyam: an ayurvedic formulation
- Phytochemical screening and toxicity studies on the methanol extract of the seeds of moringa oleifera
- First report of fingerprinting dried herbal products using a subtractive diversity array
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- Hypoglycemic activity of the Anisopus mannii N. E. Br. methanolic leaf extract in normal and alloxan-induced diabetic mice
- Aloe vera gel protects liver from oxidative stress-induced damage in experimental rat model
- Hepatoprotective activity of Calotropis gigantea flowers against carbon-tetrachloride-induced liver damage in mice
- In vivo and in vitro estrogenic activity of extracts from Erythrina poeppigiana (Fabaceae)
- Liposomal-formulated curcumin [Lipocurc™] targeting HDAC (Histone Deacetylase) prevents apoptosis and improves motor deficits in Park 7 (DJ-1)-knockout rat model of Parkinson’s disease: implications for epigenetics-based nanotechnology-driven drug platform
- Antimalarial potential of China 30 and Chelidonium 30 in combination therapy against lethal rodent malaria parasite: Plasmodium berghei
- Structure elucidation and inhibitory effects on human platelet aggregation of chlorogenic acid from Wrightia tinctoria
- Cytotoxic effects of crocin on MOLT-4 human leukemia cells
- Synergistic cytotoxicity of red beetroot (Beta vulgaris L.) extract with doxorubicin in human pancreatic, breast and prostate cancer cell lines
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