Detection of soluble suppression of tumorigenicity 2 and N-terminal B-type natriuretic peptide in a rat model of aortic regurgitation: differential responses to omecamtiv mecarbil
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Bachar El Oumeiri
,
Philippe van de Borne
Abstract
Objectives
Both N-terminal fragment of B-type natriuretic peptide (NT-proBNP) and soluble isoform of ST2 (sST2) have been identified as biomarkers of heart failure. We evaluated the plasma levels of NT-proBNP and sST2 in a rat model of severe aortic valve regurgitation (AR) and correlated these findings with echocardiographic measurements. We also examined the impact of omecamtiv mecarbil (OM) on these parameters.
Methods
The plasma levels of NT-proBNP and sST2 were measured in 18 rats both before and 2 months after surgical induction of AR, and at these same time points, in six rats assigned to a sham-procedure control group. Plasma biomarkers were then measured again after infusion of OM or placebo in rats with AR (n=8 and 10, respectively) and OM alone in the sham control rats (n=6). Echocardiographic measurements were collected before and 2 months after induction of AR.
Results
Our results revealed increased levels of plasma NT-proBNP (219 ± 34 pg/mL vs. 429 ± 374 pg/mL; p<0.001) in rats with AR at day 7 after infusion of placebo, whereas plasma levels of sST2 were higher in this cohort after infusion of either OM or placebo. We identified a significant positive correlation between plasma sST2 with posterior wall thickness in diastole (r=0.34, p<0.05) and total body weight (r=0.45, p<0.01) in rats with surgically induced AR.
Conclusions
Because sST2 increased markedly, whereas NT-proBNP remained unchanged, when OM was administered, we hypothesize that sST2 has a distinct capability to detect deleterious effects of passive muscle tension, not reliably assessed by NT-proBNP, in the setting of AR.
Funding source: Fonds pour la Chirurgie Cardiaque, Brussels, Belgium
Award Identifier / Grant number: Fondation Emile Saucez –René Van Poucke, Brusse, grant number 489639
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Research funding: This study was supported and funded by the Fonds pour la Chirurgie Cardiaque, Brussels, Belgium, grant number 489639 and Fondation Emile Saucez –René Van Poucke, Brussels, Belgium.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interest: The authors declare that they have no competing interests.
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Ethics approval: The experimental protocol was approved by the Institutional Animal Care and Use Committee of the ‘Université Libre de Bruxelles’. Studies were conducted in accordance with the Guide for the Care and Use of Laboratory Animals published by the National Institutes of Health (NIH Publication No. 85–23; revised 1996).
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Availability and data materials: All materials and data are available at request at the laboratory of physiology of the University of Brussels.
References
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© 2022 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Minireview
- The effect of endurance, resistance training, and supplements on mitochondria and bioenergetics of muscle cells
- Reviews
- Cellular cross talk between epicardial fat and cardiovascular risk
- The physiological insight of Coenzyme-Q10 administration in preventing the incidence of reperfusion arrhythmia among patients undergoing coronary artery bypass grafting surgery
- Is it possible to treat nonalcoholic liver disease using a flavanol-based nutraceutical approach? Basic and clinical data
- An overview of post COVID sequelae
- Clinical implications of anti-idiotype antibodies in COVID-19
- Original Articles
- Monocyte chemoattractant protein-1 levels are associated with major depressive disorder
- Detection of soluble suppression of tumorigenicity 2 and N-terminal B-type natriuretic peptide in a rat model of aortic regurgitation: differential responses to omecamtiv mecarbil
- Preoperative monocyte-to-lymphocyte ratio as a potential predictor of bladder cancer
- AICAR promotes endothelium-independent vasorelaxation by activating AMP-activated protein kinase via increased ZMP and decreased ATP/ADP ratio in aortic smooth muscle
- Moderate-intensity exercise decreases the circulating level of betatrophin and its correlation among markers of obesity in women
- Impact of muscle mass on blood glucose level
- Behavioral alterations, brain oxidative stress, and elevated levels of corticosterone associated with a pressure injury model in male mice
- Patterns of drug therapy, glycemic control, and predictors of escalation – non-escalation of treatment among diabetes outpatients at a tertiary care center
- Pattern, severity, and outcome of adverse drug reactions in a tertiary care hospital: an evidence from a cross-sectional study
Articles in the same Issue
- Frontmatter
- Minireview
- The effect of endurance, resistance training, and supplements on mitochondria and bioenergetics of muscle cells
- Reviews
- Cellular cross talk between epicardial fat and cardiovascular risk
- The physiological insight of Coenzyme-Q10 administration in preventing the incidence of reperfusion arrhythmia among patients undergoing coronary artery bypass grafting surgery
- Is it possible to treat nonalcoholic liver disease using a flavanol-based nutraceutical approach? Basic and clinical data
- An overview of post COVID sequelae
- Clinical implications of anti-idiotype antibodies in COVID-19
- Original Articles
- Monocyte chemoattractant protein-1 levels are associated with major depressive disorder
- Detection of soluble suppression of tumorigenicity 2 and N-terminal B-type natriuretic peptide in a rat model of aortic regurgitation: differential responses to omecamtiv mecarbil
- Preoperative monocyte-to-lymphocyte ratio as a potential predictor of bladder cancer
- AICAR promotes endothelium-independent vasorelaxation by activating AMP-activated protein kinase via increased ZMP and decreased ATP/ADP ratio in aortic smooth muscle
- Moderate-intensity exercise decreases the circulating level of betatrophin and its correlation among markers of obesity in women
- Impact of muscle mass on blood glucose level
- Behavioral alterations, brain oxidative stress, and elevated levels of corticosterone associated with a pressure injury model in male mice
- Patterns of drug therapy, glycemic control, and predictors of escalation – non-escalation of treatment among diabetes outpatients at a tertiary care center
- Pattern, severity, and outcome of adverse drug reactions in a tertiary care hospital: an evidence from a cross-sectional study
