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The effects of G protein-coupled receptor 30 (GPR30) on cardiac glucose metabolism in diabetic ovariectomized female rats

  • Mohammad Shahbazian , Faezeh Jafarynezhad , Maryam Yadeghari , Zeinab Farhadi , Sanaz Lotfi Samani , Mansour Esmailidehaj , Fatemeh Safari and Hossein Azizian ORCID logo EMAIL logo
Published/Copyright: February 15, 2022
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 34 Issue 2

Abstract

Background

Diabetic cardiometabolic disorders are characterized by significant changes in cardiac metabolism and are increased in postmenopausal women, which emphasize the role of 17β-estradiol (E2). Despite this, there are few safe and effective pharmacological treatments for these disorders. The role of G protein-coupled estrogen receptor (GPR30), which mediates the non-genomic effects of E2, is mostly unexplored.

Methods

In this study, we used ovariectomy (menopausal model) and type 2 diabetic (T2D) rats’ models to evaluate the preclinical action of G-1 (GPR30 agonist) against cardiometabolic disorders. T2D was induced by a high-fat diet and a low dose of streptozotocin. G-1 was administrated for six weeks after the establishment of T2D.

Results

We found that G-1 counteracts the effects of T2D and ovariectomy by increasing the body weight, reducing fasting blood sugar, heart weight, and heart weight to body weight ratio. Also, both ovariectomy and T2D led to decreases in the cardiac protein levels of hexokinase 2 (HK2) and GLUT4, while G-1–treated female rats reversed these changes and only increased HK2 protein level. In addition, T2D and ovariectomy increased glucose and glycogen content in the heart, but G-1 treatment significantly reduced them.

Conclusions

In conclusion, our work demonstrates that G-1 as a selective GPR30 agonist is a viable therapeutic approach against T2D and cardiometabolic diseases in multiple preclinical female models.

Keywords: diabetic heart; glucose metabolism; GPR30; ovariectomy; rats
Corresponding author: Hossein Azizian, Department of Physiology, Faculty of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran, Phone: 09137476641, Fax: +98(0)3538203410-7, E-mail:

Acknowledgments

The authors are grateful to Ms. Zeinab Hafizi for technical assistance. All authors read and approved the final manuscript.

  1. Research funding: None declared.

  2. Author contribution: All authors have accepted responsibility for the entire content of this manuscript and approved its submission

  3. Competing interests: The authors declare that there is no conflict of interests.

  4. Informed consent: Informed consent was obtained from all individuals included in this study.

  5. Ethical approval: All the experimental procedures were performed according to the committee for the purpose of control and supervision of experiments on animals, norms and approved by the Shahid Sadoughi University of Medical Sciences, Animal Ethical Committee with an approval number 1400.005

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Received: 2021-12-08
Revised: 2022-01-04
Accepted: 2022-01-25
Published Online: 2022-02-15

© 2022 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. The clinical potential of flavonoids in Peyronie’s disease
  4. Reviews
  5. Emphasizing roles of BDNF promoters and inducers in Alzheimer's disease for improving impaired cognition and memory
  6. Diabetic wound healing approaches: an update
  7. Metformin: new applications for an old drug
  8. Bispecific antibodies and its applications: a novel approach for targeting SARS-Cov-2
  9. A review on pulmonary and mediastinal synovial sarcoma
  10. Original Articles
  11. Cytokine release by human bone marrow stromal cells isolated from osteoarthritic and diabetic osteoarthritic patients in vitro
  12. Antioxidant and anti-inflammatory effect of olive leaf extract treatment in diabetic rat brain
  13. Implication of nitrergic system in the anticonvulsant effects of ferulic acid in pentylenetetrazole-induced seizures in male mice
  14. The effects of G protein-coupled receptor 30 (GPR30) on cardiac glucose metabolism in diabetic ovariectomized female rats
  15. Effects of weight-bearing vs. non-weight-bearing endurance exercise on reducing body fat and inflammatory markers in obese females
  16. [Lys5,MeLeu9,Nle10]-NKA(4–10) induces neurokinin 2 receptor mediated urination and defecation and neurokinin 1 receptor mediated flushing in rats: measured using the rapid detection voiding assay
  17. Single 30 min treadmill exercise session suppresses the production of pro-inflammatory cytokines and oxidative stress in obese female adolescents
  18. Letter to the Editor
  19. Effect of tirzepatide on prediabetics and blood pressure with implications for future research
https://doi.org/10.1515/jbcpp-2021-0374
Keywords for this article
diabetic heart; glucose metabolism; GPR30; ovariectomy; rats

Articles in the same Issue

  1. Frontmatter
  2. Editorial
  3. The clinical potential of flavonoids in Peyronie’s disease
  4. Reviews
  5. Emphasizing roles of BDNF promoters and inducers in Alzheimer's disease for improving impaired cognition and memory
  6. Diabetic wound healing approaches: an update
  7. Metformin: new applications for an old drug
  8. Bispecific antibodies and its applications: a novel approach for targeting SARS-Cov-2
  9. A review on pulmonary and mediastinal synovial sarcoma
  10. Original Articles
  11. Cytokine release by human bone marrow stromal cells isolated from osteoarthritic and diabetic osteoarthritic patients in vitro
  12. Antioxidant and anti-inflammatory effect of olive leaf extract treatment in diabetic rat brain
  13. Implication of nitrergic system in the anticonvulsant effects of ferulic acid in pentylenetetrazole-induced seizures in male mice
  14. The effects of G protein-coupled receptor 30 (GPR30) on cardiac glucose metabolism in diabetic ovariectomized female rats
  15. Effects of weight-bearing vs. non-weight-bearing endurance exercise on reducing body fat and inflammatory markers in obese females
  16. [Lys5,MeLeu9,Nle10]-NKA(4–10) induces neurokinin 2 receptor mediated urination and defecation and neurokinin 1 receptor mediated flushing in rats: measured using the rapid detection voiding assay
  17. Single 30 min treadmill exercise session suppresses the production of pro-inflammatory cytokines and oxidative stress in obese female adolescents
  18. Letter to the Editor
  19. Effect of tirzepatide on prediabetics and blood pressure with implications for future research
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