Cytokine release by human bone marrow stromal cells isolated from osteoarthritic and diabetic osteoarthritic patients in vitro
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Kar Wai Loh
and
Tunku Kamarul
Abstract
Objectives
Primary Osteoarthritis (OA) is a disease of progressive joints degeneration due to idiopathic causes. Recent evidence showed a positive relationship between OA and metabolic syndrome. This pilot study aimed to assess the baseline level of pro and anti-inflammatory cytokines in OA patients with or without Diabetic Mellitus (DM) and assess the effect of hydrogen peroxide (H2O2) in cytokine production.
Methods
Patients with primary hip and knee OA were recruited, and 3 mL of bone marrow was harvested during joint replacement surgery. Bone marrow stromal cells (BMSC) was isolated and cultured in a culture flask for three passages. Later experiment was then sub-cultured in a well plate labeled as the control group and H2O2 (0.1 mM) treated group. ProcartaPlex® Multiplex Immunoassay was performed to measure cytokine levels produced by the BMSC at 0 h, as well as 72 h.
Results
Cytokines such as tumor necrosis factor-alpha, interleukin (IL)-6, IL-8, and IL-1β generally exhibited higher cytokine levels in subjects with DM than in nonDM subjects at 0 and 72 h. For IL-17, its expression was similar in nonDM and DM groups at 0 and 72 h. Cytokine IL-10 showed no significant difference in both the groups while DM and nonDM groups treated with H2O2 showed decreased IL-4 levels compared to control groups at 72 h. Bone marrow cells from DM-OA are more vulnerable to chemical insult and are associated with higher levels of proinflammatory cytokines production and lower IL-4 level production.
Conclusions
This study provides a clue that management of OA with co-morbidity like DM needs future studies.
Funding source: Fundamental Research Grant Scheme Ministry of Higher Education
Award Identifier / Grant number: FRGS/1/2016/STG03/UM/02/2
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Research funding: This study was supported by Fundamental Research Grant (FRGS/1/2016/STG03/UM/02/2).
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Informed consent was obtained from all individuals included in this study.
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Ethical approval: Medical ethics of this study was approved and granted by the Medical Research Ethics Committee (MREC) of the University of Malaya Medical Center (UMMC) with the reference number: 2018713-6483.
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Editorial
- The clinical potential of flavonoids in Peyronie’s disease
- Reviews
- Emphasizing roles of BDNF promoters and inducers in Alzheimer's disease for improving impaired cognition and memory
- Diabetic wound healing approaches: an update
- Metformin: new applications for an old drug
- Bispecific antibodies and its applications: a novel approach for targeting SARS-Cov-2
- A review on pulmonary and mediastinal synovial sarcoma
- Original Articles
- Cytokine release by human bone marrow stromal cells isolated from osteoarthritic and diabetic osteoarthritic patients in vitro
- Antioxidant and anti-inflammatory effect of olive leaf extract treatment in diabetic rat brain
- Implication of nitrergic system in the anticonvulsant effects of ferulic acid in pentylenetetrazole-induced seizures in male mice
- The effects of G protein-coupled receptor 30 (GPR30) on cardiac glucose metabolism in diabetic ovariectomized female rats
- Effects of weight-bearing vs. non-weight-bearing endurance exercise on reducing body fat and inflammatory markers in obese females
- [Lys5,MeLeu9,Nle10]-NKA(4–10) induces neurokinin 2 receptor mediated urination and defecation and neurokinin 1 receptor mediated flushing in rats: measured using the rapid detection voiding assay
- Single 30 min treadmill exercise session suppresses the production of pro-inflammatory cytokines and oxidative stress in obese female adolescents
- Letter to the Editor
- Effect of tirzepatide on prediabetics and blood pressure with implications for future research
Articles in the same Issue
- Frontmatter
- Editorial
- The clinical potential of flavonoids in Peyronie’s disease
- Reviews
- Emphasizing roles of BDNF promoters and inducers in Alzheimer's disease for improving impaired cognition and memory
- Diabetic wound healing approaches: an update
- Metformin: new applications for an old drug
- Bispecific antibodies and its applications: a novel approach for targeting SARS-Cov-2
- A review on pulmonary and mediastinal synovial sarcoma
- Original Articles
- Cytokine release by human bone marrow stromal cells isolated from osteoarthritic and diabetic osteoarthritic patients in vitro
- Antioxidant and anti-inflammatory effect of olive leaf extract treatment in diabetic rat brain
- Implication of nitrergic system in the anticonvulsant effects of ferulic acid in pentylenetetrazole-induced seizures in male mice
- The effects of G protein-coupled receptor 30 (GPR30) on cardiac glucose metabolism in diabetic ovariectomized female rats
- Effects of weight-bearing vs. non-weight-bearing endurance exercise on reducing body fat and inflammatory markers in obese females
- [Lys5,MeLeu9,Nle10]-NKA(4–10) induces neurokinin 2 receptor mediated urination and defecation and neurokinin 1 receptor mediated flushing in rats: measured using the rapid detection voiding assay
- Single 30 min treadmill exercise session suppresses the production of pro-inflammatory cytokines and oxidative stress in obese female adolescents
- Letter to the Editor
- Effect of tirzepatide on prediabetics and blood pressure with implications for future research
