Startseite Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70
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Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70

  • Sadanandan Rajapriya und Arumugam Geetha EMAIL logo
Veröffentlicht/Copyright: 24. Juni 2021
Journal of Basic and Clinical Physiology and Pharmacology
Aus der Zeitschrift Journal of Basic and Clinical Physiology and Pharmacology Band 33 Heft 4

Abstract

Objectives

Nod-like receptor pyrin domain containing 3 (NLRP3) is one of the well characterized inflammasome that controls the maturation of pro-inflammatory cytokines and thereby the inflammation in pancreas which could be a promising target for anti-inflammatory drugs. The present study is aimed to explore whether luteolin can target the NLRP3 inflammasome and modulate its activity through the signaling protein, HSP70 in the ethanol-cerulein model of experimental pancreatitis.

Methods

Male albino Wistar rats were divided into four groups. Groups 1 and 2 rats received normal diet. Groups 3 and 4 rats received isocalorically adjusted diet containing ethanol for 5 weeks and cerulein (20 μg/kg body weight i.p., thrice weekly for the last 3 weeks of the experimental period). Additionally, group 2 and 4 rats received 2 mg/kg body weight of luteolin orally from third week.

Results

Luteolin co-administration decreased the serum levels of HSP70, oxidative stress markers, myeloperoxidase, GSH/GSSG and GST with concomitant downregulation in the mRNA expression of HSP70, caspase-1, ASC-NLRP3 and NF-κB. Spearman’s rank correlation test showed that serum HSP70 has positive correlation with the expression of ASC-NLRP3, caspase-1, NF-κB and 4-hydroxynonenal and negative correlation with GSH:GSSG ratio.

Conclusions

The modulating effect of luteolin on the expression of HSP70, NF-κB and thereby on ASC-NLRP3 complex may be claimed for its pancreato-protective activity.

Keywords: heat shock protein 70; luteolin; oxidative stress; pancreatitis
Corresponding author: Arumugam Geetha, Dr. Ambedkar Government Arts College, Chennai 600 039, Tamil Nadu, India, Phone: +91 7904316378, E-mail:

Funding source: Indian Council of Medical Research 10.13039/501100001411

Award Identifier / Grant number: 45/07/2018-BIO/BMS

Acknowledgments

The authors thank Dr. Pramod K. Srivastava, Professor of Immunology and Medicine and Director, Carole and Ray Neag Comprehensive Cancer Center, UConn School of Medicine, Farmington, Connecticut, USA for his generous gift of ELISA kit.

  1. Research funding: Financial support was provided by Indian Council of Medical Research (ICMR) [Senior Research Fellow, File no.: 45/07/2018-BIO/BMS], New Delhi, India.

  2. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  3. Competing interests: The funding organization(s) played no role in the study design, in the collection, analysis and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.

  4. Ethical approval: This study was approved by the Committee for the purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India, New Delhi, (XVII/VELS/PCOL/14/2000/CPCSEA/IAEC/06.10.15).

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Received: 2020-08-11
Accepted: 2021-04-14
Published Online: 2021-06-24

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Minireview
  3. Can pulse wave velocity (PWV) alone express arterial stiffness? A neglected tool for vascular function assessment
  4. Review
  5. Hyponatremia in heart failure: not just 135 to 145
  6. Original Articles
  7. Effects of berberine on cholinesterases and monoamine oxidase activities, and antioxidant status in the brain of streptozotocin (STZ)-induced diabetic rats
  8. Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats
  9. Terminalia arjuna supplementation ameliorates high fat diet-induced oxidative stress in nephrotoxic rats
  10. Decreased expression of annexin A2 and loss of its association with vascular endothelial growth factor leads to the deficient trophoblastic invasion in preeclampsia
  11. Algogen-induced vasosensory reflexes modulate short-term heart rate variability parameters in experimental rat models
  12. Assessment of sleep quality and its predictors among newly diagnosed psychiatric patients
  13. Ovalbumin/lipopolysaccharide induced vasculitis in rats: a new predictive model
  14. Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats
  15. Effects of sleeve gastrectomy on neutrophil–lymphocyte ratio
  16. Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70
  17. Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line
  18. Feasibility, face, and content validity of quantitative computed tomography in interstitial lung disease related to connective tissue diseases
  19. In vitro anticoagulant activity of selected medicinal plants: potential interactions with warfarin and development of new anticoagulants
  20. Normal reference value of orthodromic and antidromic sensory nerve conduction velocity of median nerve with intact palmaris longus tendon in apparently healthy individuals
  21. Short Communication
  22. Chemokine receptor antagonists with α1-adrenergic receptor blocker activity
https://doi.org/10.1515/jbcpp-2020-0255
Schlagwörter für diesen Artikel
heat shock protein 70; luteolin; oxidative stress; pancreatitis

Artikel in diesem Heft

  1. Frontmatter
  2. Minireview
  3. Can pulse wave velocity (PWV) alone express arterial stiffness? A neglected tool for vascular function assessment
  4. Review
  5. Hyponatremia in heart failure: not just 135 to 145
  6. Original Articles
  7. Effects of berberine on cholinesterases and monoamine oxidase activities, and antioxidant status in the brain of streptozotocin (STZ)-induced diabetic rats
  8. Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats
  9. Terminalia arjuna supplementation ameliorates high fat diet-induced oxidative stress in nephrotoxic rats
  10. Decreased expression of annexin A2 and loss of its association with vascular endothelial growth factor leads to the deficient trophoblastic invasion in preeclampsia
  11. Algogen-induced vasosensory reflexes modulate short-term heart rate variability parameters in experimental rat models
  12. Assessment of sleep quality and its predictors among newly diagnosed psychiatric patients
  13. Ovalbumin/lipopolysaccharide induced vasculitis in rats: a new predictive model
  14. Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats
  15. Effects of sleeve gastrectomy on neutrophil–lymphocyte ratio
  16. Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70
  17. Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line
  18. Feasibility, face, and content validity of quantitative computed tomography in interstitial lung disease related to connective tissue diseases
  19. In vitro anticoagulant activity of selected medicinal plants: potential interactions with warfarin and development of new anticoagulants
  20. Normal reference value of orthodromic and antidromic sensory nerve conduction velocity of median nerve with intact palmaris longus tendon in apparently healthy individuals
  21. Short Communication
  22. Chemokine receptor antagonists with α1-adrenergic receptor blocker activity
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