Terminalia arjuna supplementation ameliorates high fat diet-induced oxidative stress in nephrotoxic rats
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Pallavi S. Kanthe
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Bheemshetty S. Patil
Abstract
Objectives
Dietary high fat possibly causes oxidative stress; also it makes deleterious effect on kidney functions and land up in lipotoxicity in renal tissue. Lipotoxicity is the pathological situation where lipid Peroxidation occurs and generates reactive oxygen species (ROS). Overproduction of ROS than antioxidant present in tissues cause oxidative stress. Terminalia arjuna is found to be potential antioxidant that counteract oxidative stress and possibly maintain glomerular integrity.
Methods
Ethanolic extract of T. arjuna (ETO) was prepared and phytochemical analysis was done. Rats were divided into four groups, having six rats in each group as following; group 1-Control (20% fat); group 2 (ETO 100 mg/kg/b.wt); group 3 (30% fat) and group 4 (30% fat + ETO 100 mg/kg/b.wt). Dietary and ETO supplementation were continued for 21 days. Gravimetric, kidney functions (blood urea and serum creatinine) and oxidative stress markers like MDA, SOD and GSH were evaluated. Histopathological analysis was done on kidney along with measurement of glomerular integrity. Morphometrical analysis of glomerular integrity was evaluated by measuring glomerular length, width, glomerular area and Bowman’s capsule radius. One way ANOVA was done for analysis of data.
Results
Blood urea and serum creatinine levels were significantly higher in high fat fed rats indicating renal dysfunction. High fat diet showed significant increase in MDA, decrease in SOD and GSH in rats fed with high fat diet, which indicate generation of oxidative stress. Supplementation of ETO showed amelioratic effect against high fat diet induced renal dysfunction and oxidative stress. Histopathological findings were significantly corroborated with morphometrical analysis of glomerular integrity.
Conclusions
Ethanolic extracts of T. arjuna supplementation found to be beneficial against high fat induced renal alterations in terms of functions and architecture.
Acknowledgments
The authors greatly acknowledge BLDE (DU), Vijayapura, and Karnataka, India, to carry out this work.
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Research funding: None disclosed.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Informed consent: Not applicable.
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Ethical approval: All the experimental procedures were performed in accordance with the approval of the Institutional Animal Ethics Committee (IAEC; Ref No. 268/11 dated 01/06/2011) according to the guidelines of the Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA), Government of India.
References
1. Patil, BS, Kanthe, PS, Reddy, CR, Das, KK. Emblica officinalis (Amla) Ameliorates high-fat diet induced Alteration of cardiovascular pathophysiology. Cardiovasc Hematol Agents Med Chem 2019;17:52–63. https://doi.org/10.2174/1871525717666190409120018.Search in Google Scholar
2. Kanthe, PS, Patil, BS, Khodnapur, JP, Bagali, SC, Mullur, L, Aithala, M. Comparative study of rate pressure product in obese women with non-obese women. Int J Biomed Adv Res 2012;3:580–3. https://doi.org/10.7439/ijbar.v3i7.569.Search in Google Scholar
3. Ding, S, Fan, Y, Zhao, N, Yang, H, Ye, X, He, D, et al.. High fat aggravates glucose homeostasis by chronic exposure to bisphenol A. J Endocrinol 2014;221:167–79. https://doi.org/10.1530/joe-13-0386.Search in Google Scholar
4. Kanthe, PS, Patil, BS, Bagali, SC, Reddy, RC, Aithala, MR, Das, KK. Protective effects of ethanolic extract of Emblica officinalis (Amla) on cardiovascular pathophysiology of rats, fed with high fat diet. J Clin Diagn Res: JCDR. 2017;11:CC05. https://doi.org/10.7860/jcdr/2017/28474.10628.Search in Google Scholar
5. Salim, HM, Kurnia, LF, Bintarti, TW, Handayani, H. The effects of high-fat diet on histological changes of kidneys in rats. Biomol Health Sci J 2018;1:109–12. https://doi.org/10.20473/bhsj.v1i2.9675.Search in Google Scholar
6. Muller, CR, Leite, AP, Yokota, R, Pereira, RO, Americo, AL, Nascimento, NR, et al.. Post-weaning exposure to high-fat diet induces kidney lipotoxicity in Adult rats. Frontiers in nutrition 2019;6:60. https://doi.org/10.3389/fnut.2019.00060.Search in Google Scholar
7. Jasmine, B, Arun, K, Christopher, M. Diet-induced metabolic hamster model of nonalcoholic fatty liver disease. Diabetes Metab Syndr Obes 2011;4:195–203.10.2147/DMSO.S18435Search in Google Scholar
8. Mohanty, IR, Borde, M, Maheshwari, U. Dipeptidyl peptidase IV Inhibitory activity of Terminalia arjuna attributes to its cardioprotective effects in experimental diabetes: in silico, in vitro and in vivo analyses. Phytomedicine 2019;57:158–65. https://doi.org/10.1016/j.phymed.2018.09.195.Search in Google Scholar
9. Uthirapathy, S. Novel biomarkers of atherogenic diet induced dyslipidemia and metabolic syndrome suppressed by Terminalia arjuna. Int J Pharma Sci Res 2019;10:2528–36. https://doi.org/10.13040/IJPSR.0975-8232.10(5).2528-36.Search in Google Scholar
10. Barman, S, Das, S. Hypoglycemic effect of ethanolic extract of bark of Terminalia arjuna Linn. in normal and alloxan-induced noninsulin-dependent diabetes mellitus albino rats. Int J Green Pharm 2012;6. https://doi.org/10.4103/0973-8258.108207.Search in Google Scholar
11. Dwivedi, S, Chopra, D. Revisiting Terminalia arjuna–an ancient cardiovascular drug. J Tradit Complement Med 2014;4:224–31. https://doi.org/10.4103/2225-4110.139103.Search in Google Scholar PubMed PubMed Central
12. Manu, TM, Anand, T, Pandareesh, MD, Kumar, PB, Khanum, F. Terminalia arjuna extract and arjunic acid mitigate cobalt chloride–induced hypoxia stress–mediated apoptosis in H9c2 cells. N Schmied Arch Pharmacol 2019;392:1107–19. https://doi.org/10.1007/s00210-019-01654-x.Search in Google Scholar
13. Amalraj, A, Gopi, S. Medicinal properties of Terminalia arjuna (Roxb.) Wight & Arn.: a review. J Tradit Complement Med 2017;7:65–78. https://doi.org/10.1016/j.jtcme.2016.02.003.Search in Google Scholar
14. Tiwari, P, Kumar, B, Kaur, M, Kaur, G, Kaur, H. Phytochemical screening and extraction: A review. Int Pharm Sci 2011;1:103–4.Search in Google Scholar
15. Das, KK, Dasgupta, S. Effect of nickel sulfate on testicular steroidogenesis in rats during protein restriction. Environ Health Perspect 2002;110:923–6. https://doi.org/10.1289/ehp.02110923.Search in Google Scholar
16. Vasanthi, P, Parameswari, CS. Aqueous extract of Terminalia arjuna prevents cyclosporine-induced renal disorders. Comp Clin Pathol 2014;23:583–8. https://doi.org/10.1007/s00580-012-1655-7.Search in Google Scholar
17. Khanna, AK, Chander, R, Kapoor, NK. Terminalia arjuna: an ayurvedic cardiotonic, regulates lipid metabolism in hyperlipaemic rats. Phytother Res 1996;10:663–5. https://doi.org/10.1002/(sici)1099-1573(199612)10:8<663::aid-ptr935>3.0.co;2-w.10.1002/(SICI)1099-1573(199612)10:8<663::AID-PTR935>3.0.CO;2-WSearch in Google Scholar
18. Okhawa, H, Ohishi, N, Yagi, K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351–8.10.1016/0003-2697(79)90738-3Search in Google Scholar
19. Buege, JA, Aust, SD. Microsomal lipid peroxidation. Methods Enzymol 1978;52:302–10. https://doi.org/10.1016/s0076-6879(78)52032-6.Search in Google Scholar
20. Marklund, Marklund, G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. EJB 1974;47:469–47. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x.Search in Google Scholar
21. Beutler, E, Duron, O, Kelly, BM. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882–8.Search in Google Scholar
22. Shetty, B, Rao, G, Banu, N, Reddy, S. Study of protective action of Spondias pinnata bark extract on rat liver and kidney against etoposide induced chemical stress. Phcog J 2016;8.10.5530/pj.2016.1.5Search in Google Scholar
23. Kostyuk, VA, Potapovich, AI, Kovaleva, JI. Simple and sensitive method of definition of superoxide dismutase, based on reaction of oxidation of kvercetine. Questions Med Chem 1990;2:88–91.Search in Google Scholar
24. Luna, L. Manual of histologic staining methods of the AFIP, 3rd ed. NY: McGraw-Hill; 1968:76 p.Search in Google Scholar
25. Available from:https://www.micro-shop.zeiss.com/?l=en&p=hr&f=e&i=10290&o=&h=25&n=0&sd=410135-1019-200 [Accessed 26 Feb 2015].Search in Google Scholar
26. Panigrahi, T, Vishwas, S, Dash, D. A herbal approach to obesity Management: a review. Asian J Pharmaceut Educ Res 2017;6:1–5.Search in Google Scholar
27. Stemmer, K, Perez, TD, Ananthkrishnan, G, Bort, A, Seeley, RJ, Tschöp, MH, et al.. High fat diet induced obesity causes an inflammation and tumor promoting microenvironment in the rat kidney. Dis Model Mech 2012;5:627–35. https://doi.org/10.1242/dmm.009407.Search in Google Scholar
28. Das, K, Chakraborty, PP, Ghosh, D, Nandi, DK. Protective effect of aqueous extract of Terminalia arjuna against dehydrating induced oxidative stress and uremia in male rat. Iran J Pharm Res (IJPR): IJPR 2010;9:153.Search in Google Scholar
29. Ragavan, B, Krishnakumari, S. Effect of T. arjuna stem bark extract on histopathology of liver, kidney and pancreas of alloxan-induced diabetic rats. Afr J Biomed Res 2006;9.10.4314/ajbr.v9i3.48904Search in Google Scholar
30. Raj, CD, Shabi, MM, Jipnomon, J, Dhevi, R, Gayathri, K, Subashini, U, et al.. Terminalia arjuna’s antioxidant effect in isolated perfused kidney. Research in pharmaceutical sciences 2012;7:181.Search in Google Scholar
31. Garcia, IJ, Cézar, JS, Lemos, BS, Silva, LN, Ribeiro, RI, Santana, CC, et al.. Effects of high fat diet on kidney lipid content and the Na, K-ATPase activity. Braz J Pharmaceut Sci 2018;54. https://doi.org/10.1590/s2175-97902018000117165.Search in Google Scholar
32. Dutta, MO, Ghosh, AK, Basu, AN, Bandyopadhyay, DE, Chattopadhyay, AI. Protective effect of aqueous bark extract of Terminalia arjuna against copper ascorbate induced oxidative stress in vitro in goat heart mitochondria. Int J Pharm Pharmaceut Sci 2013;5:439–47.10.1039/C4FO00355ASearch in Google Scholar
33. Mishra, S, Naaz, S, Ghosh, AK, Paul, S, Ghosal, N, Dutta, M, et al.. Orally administered aqueous bark extract of Terminalia arjuna protects against adrenaline-induced myocardial injury in rat heart through antioxidant mechanisms: an in vivo and an in vitro study. J Pharm Res 2016;10:454–78.Search in Google Scholar
34. Manna, P, Sinha, M, Sil, PC. Aqueous extract of Terminalia arjuna prevents carbon tetrachloride induced hepatic and renal disorders. BMC Complement Altern Med 2006;6:33. https://doi.org/10.1186/1472-6882-6-33.Search in Google Scholar PubMed PubMed Central
35. Priya, N, Mathur, KC, Sharma, A, Agarwal, V, Acharya, J. Effect of Terminalia arjuna on total platelet count. Adv Hum Biol 2019;9:98–101.10.4103/AIHB.AIHB_8_18Search in Google Scholar
36. Wicks, SE, Nguyen, TT, Breaux, C, Kruger, C, Stadler, K. Diet-induced obesity and kidney disease–In search of a susceptible mouse model. Biochimie 2016;124:65–73. https://doi.org/10.1016/j.biochi.2015.08.001.Search in Google Scholar PubMed PubMed Central
37. Van Der Heijden, RA, Bijzet, J, Meijers, WC, Yakala, GK, Kleemann, R, Nguyen, TQ, et al.. Obesity-induced chronic inflammation in high fat diet challenged C57BL/6J mice is associated with acceleration of age-dependent renal amyloidosis. Sci Rep 2015;5:16474. https://doi.org/10.1038/srep16474.Search in Google Scholar PubMed PubMed Central
38. George, AL, Nelson, EG. Disorders of kidney and urinary tract. In: Kasper, DL, Fauci, AS, Hauser, S, et al., editors. Harrison’s principles of internal medicine, 17th ed. New York: McGraw-Hill Medical; 2008, vol 9:98–101 pp.Search in Google Scholar
39. Borde, MK, Mohanty, IR, Maheshwari, U, Suman, RK, Deshmukh, YA, et al.. Natural dipeptidyl peptidase-4 inhibitor Terminalia arjuna mitigates myocardial infarction co-existing with diabetes in experimental rats. J Diabetes Metab Disord Control 2018;5:48–56.10.15406/jdmdc.2018.05.00137Search in Google Scholar
© 2021 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Minireview
- Can pulse wave velocity (PWV) alone express arterial stiffness? A neglected tool for vascular function assessment
- Review
- Hyponatremia in heart failure: not just 135 to 145
- Original Articles
- Effects of berberine on cholinesterases and monoamine oxidase activities, and antioxidant status in the brain of streptozotocin (STZ)-induced diabetic rats
- Paradoxical sleep deprivation induces oxidative stress in the submandibular glands of Wistar rats
- Terminalia arjuna supplementation ameliorates high fat diet-induced oxidative stress in nephrotoxic rats
- Decreased expression of annexin A2 and loss of its association with vascular endothelial growth factor leads to the deficient trophoblastic invasion in preeclampsia
- Algogen-induced vasosensory reflexes modulate short-term heart rate variability parameters in experimental rat models
- Assessment of sleep quality and its predictors among newly diagnosed psychiatric patients
- Ovalbumin/lipopolysaccharide induced vasculitis in rats: a new predictive model
- Dose-dependent and time-dependent metabolic, hemodynamic, and redox disturbances in dexamethasone-treated Wistar rats
- Effects of sleeve gastrectomy on neutrophil–lymphocyte ratio
- Effect of luteolin on the gene level expression of apoptosis-associated speck-like protein containing a caspase recruitment domain of NLRP3 inflammasome and NF-κB in rats subjected to experimental pancreatitis – influence of HSP70
- Effects of Onosma dichroanthum Boiss. root extract on AGS human gastric cancer cell-line
- Feasibility, face, and content validity of quantitative computed tomography in interstitial lung disease related to connective tissue diseases
- In vitro anticoagulant activity of selected medicinal plants: potential interactions with warfarin and development of new anticoagulants
- Normal reference value of orthodromic and antidromic sensory nerve conduction velocity of median nerve with intact palmaris longus tendon in apparently healthy individuals
- Short Communication
- Chemokine receptor antagonists with α1-adrenergic receptor blocker activity
