Startseite Intermittent exposure to green and white light-at-night activates hepatic glycogenolytic and gluconeogenetic activities in male Wistar rats
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Intermittent exposure to green and white light-at-night activates hepatic glycogenolytic and gluconeogenetic activities in male Wistar rats

  • Abayomi O. Ige ORCID logo EMAIL logo , Olubori S. Adekanye und Elsie O. Adewoye
Veröffentlicht/Copyright: 21. Juni 2021
Journal of Basic and Clinical Physiology and Pharmacology
Aus der Zeitschrift Journal of Basic and Clinical Physiology and Pharmacology Band 34 Heft 4

Abstract

Objectives

Exposure to light-at-night (LAN) has been reported to impair blood glucose regulation. The liver modulates blood glucose through mechanisms influenced by several factors that include peroxisome proliferator-activated receptor gamma coactivator-1alpha (PGC-1α) and glucose-6-phosphatase (G6Pase). This study investigated the effect of intermittent exposure to green and white LAN on some hepatic glucose regulatory factors in male Wistar rats.

Methods

Animals were divided into three equal groups. Group I (control) was exposed to normal housing conditions. Groups II and III were each daily exposed to either green or white LAN for 2 h (7–9 pm) for 14 days. Body weight and blood glucose was monitored on days 0, 7, and 14. Thereafter, retro-orbital sinus blood was obtained after light thiopental anaesthesia and serum insulin was determined. Liver samples were also obtained and evaluated for glycogen, PGC-1α, and G6Pase activity. Insulin resistance was estimated using the HOMA-IR equation.

Results

Body weight and blood glucose on days 7 and 14 increased in groups II and III compared to control. Hepatic PGC-1α and G6Pase increased in group II (2.33 ± 0.31; 2.07 ± 0.22) and III (2.31 ± 0.20; 0.98 ± 0.23) compared to control (1.73 ± 0.21; 0.47 ± 0.11). Hepatic glycogen was 71.8 and 82.4% reduced in groups II and III compared to control. Insulin in group II increased (63.6%) whiles group III values reduced (27.3%) compared to control. Insulin resistance increased in group II (0.29 ± 0.09) compared to control (0.12 ± 0.03) and group III (0.11 ± 0.03), respectively.

Conclusions

Exposure to 2 h green and white LAN in the early dark phase increases hepatic glycogenolysis and gluconeogenetic activities resulting in increased blood glucose. In male Wistar rats, exposure to green but not white LAN may predispose to insulin resistance.

Keywords: green light; hepatic glucose homeostasis; light-at-night; liver; white light
Corresponding author: Abayomi O. Ige, Applied and Environmental Physiology Unit, Department of Physiology, University of Ibadan, Ibadan, Nigeria, Phone: +234 8033787617, E-mail:

Acknowledgements

The authors would like the acknowledge Dr. S.T Shittu of the Department of Physiology for some technical support in the course of this study.

  1. Research funding: None declared.

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: For this research related to animals, the Applied and Environmental Physiology Unit, Department of Physiology University of Ibadan approved the experimental procedures and protocol, which has complied with all the guidelines laid down by the Animal Care and Use Research Ethics Committee (ACUREC), University of Ibadan and that of the Guide for the Care and Use of Laboratory Animals, 1996, published by National Academy Press, 2101 Constitution Ave. NW, Washington, DC 20055, USA (The Applied and Environmental Unit, Department of Physiology does not issue approval numbers. However, all research studies emanating from this Unit is scrutinised with emphasis on humane care and treatment of animals. Furthermore, the Unit also ensures that all research projects are presented to a larger audience consisting of experts in the field Physiological research. Letters supporting this can be obtained from the Department of Physiology, University of Ibadan if required).

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Received: 2020-09-09
Accepted: 2021-04-15
Published Online: 2021-06-21

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

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  2. Minireview
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https://doi.org/10.1515/jbcpp-2020-0251
Schlagwörter für diesen Artikel
green light; hepatic glucose homeostasis; light-at-night; liver; white light

Artikel in diesem Heft

  1. Frontmatter
  2. Minireview
  3. Impact of mental toughness on athlete’s performance and interventions to improve
  4. Reviews
  5. A review of the mechanisms of anti-cancer activities of some medicinal plants–biochemical perspectives
  6. Antimicrobials in COVID-19: strategies for treating a COVID-19 pandemic
  7. Anxiolytic effects of vestibular stimulation: an update
  8. Original Articles
  9. Intermittent exposure to green and white light-at-night activates hepatic glycogenolytic and gluconeogenetic activities in male Wistar rats
  10. Study of pre-operative neutrophil–lymphocyte ratio in urothelial carcinoma
  11. The effects of Berberis vulgaris L. root extract on the opiate withdrawal syndrome and psychological factors: a randomized double-blind clinical trial
  12. Evidence of alterations in the learning and memory in offspring of stress-induced male rats
  13. The impact of vestibular symptoms and electronystagmography results on recovery from sudden sensorineural hearing loss
  14. Complementary mechanisms of modulation of spontaneous phasic contractions by the gaseous signalling molecules NO, H2S, HNO and the polysulfide Na2S3 in the rat colon
  15. Pharmacist-directed Sputnik V (GAM-COVID-VAC) surveillance program: a prospective observational study in Southern India
  16. Correlation among Poincare plot and traditional heart rate variability indices in adults with different risk levels of metabolic syndrome: a cross-sectional approach from Southern India
  17. Does etodolac affect TRPA1 functionality in vivo in human?
  18. Dynamic of irisin secretion change after moderate-intensity chronic physical exercise on obese female
  19. Letter to the Editor
  20. The prospective record-breaking obesity drug tirzepatide raises concerns about affordability
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