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Complementary mechanisms of modulation of spontaneous phasic contractions by the gaseous signalling molecules NO, H2S, HNO and the polysulfide Na2S3 in the rat colon

  • Ervice Pouokam ORCID logo , Adriana Vallejo , Emma Martínez , Sara Traserra and Marcel Jimenez EMAIL logo
Published/Copyright: October 8, 2021
Journal of Basic and Clinical Physiology and Pharmacology
From the journal Journal of Basic and Clinical Physiology and Pharmacology Volume 34 Issue 4

Abstract

Objectives

Reactive oxygen and nitrogen species may be produced during inflammation leading to the formation of NO, H2S or HNO. Enzymes such as iNOS, CSE and CBS might also be responsible for polysulfide production. Since these signalling molecules might have an impact on colonic motility, the aim of this study was to compare their effect on rat colonic slow phasic contractions (SPC).

Methods

Organ bath measurements with strips obtained from rat proximal colon were performed using the polysulfide Na2S3, sodium nitroprusside (NaNP), sodium hydrogen sulfide (NaHS), Angeli’s salt as NO, H2S, and HNO donors, respectively. TTX (1 µM) was used to block neuronal activity.

Results

All four molecules, concentration-dependently, inhibited the amplitude and frequency of SPC both in the circular and longitudinal muscle layer. The relative potency was NaNP>Angeli’s salt>NaHS>Na2S3. The inhibitory response induced by NaNP (1 µM) and Angeli’s salt (50 µM) was reversed by ODQ (10 µM) whereas the inhibitory effect of NaHS (1 mM) was reversed by apamin (1 µM) and glibenclamide (10 µM). Na2S3 (1 mM) response was partially reversed by apamin (1 µM) and glibenclamide (10 µM). High concentrations of Na2S3 caused an increase in tone. Low concentrations of NaHS or Na2S3 did not potentiate NaNP responses.

Conclusions

All signalling molecules inhibit SPC in both muscle layers. The effect is independent of neural activity and involves guanylyl cyclase (NO and HNO) and SKCa and KATP channels (NaHS or Na2S3). Other pathways might also be involved in Na2S3 responses. Accordingly, complementary mechanisms of inhibition might be attributable to these signalling molecules.

Keywords: gasotransmitter; slow phasic contractions; small conductance Ca2+-activated K+ channels (SKca); soluble guanylyl cyclase
Corresponding author: Marcel Jimenez, Ph.D, Department of Cell Biology, Physiology and Immunology and Neurosciences Institute, Universitat Autònoma de Barcelona, Barcelona, Spain, Phone: 34 935811566, E-mail:

Funding source: German Research Foundation

Award Identifier / Grant number: PO 2143/1-1

  1. Research funding: This study was supported by a research fund from the German Research Foundation (PO 2143/1-1).

  2. Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission. All five authors performed the experiments and analyzed the data; Ervice Pouokam and Marcel Jimenez designed the experiments and wrote the paper.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: Not applicable.

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Received: 2021-06-16
Revised: 2021-09-24
Accepted: 2021-09-26
Published Online: 2021-10-08

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jbcpp-2021-0181
Keywords for this article
gasotransmitter; slow phasic contractions; small conductance Ca2+-activated K+ channels (SKca); soluble guanylyl cyclase

Articles in the same Issue

  1. Frontmatter
  2. Minireview
  3. Impact of mental toughness on athlete’s performance and interventions to improve
  4. Reviews
  5. A review of the mechanisms of anti-cancer activities of some medicinal plants–biochemical perspectives
  6. Antimicrobials in COVID-19: strategies for treating a COVID-19 pandemic
  7. Anxiolytic effects of vestibular stimulation: an update
  8. Original Articles
  9. Intermittent exposure to green and white light-at-night activates hepatic glycogenolytic and gluconeogenetic activities in male Wistar rats
  10. Study of pre-operative neutrophil–lymphocyte ratio in urothelial carcinoma
  11. The effects of Berberis vulgaris L. root extract on the opiate withdrawal syndrome and psychological factors: a randomized double-blind clinical trial
  12. Evidence of alterations in the learning and memory in offspring of stress-induced male rats
  13. The impact of vestibular symptoms and electronystagmography results on recovery from sudden sensorineural hearing loss
  14. Complementary mechanisms of modulation of spontaneous phasic contractions by the gaseous signalling molecules NO, H2S, HNO and the polysulfide Na2S3 in the rat colon
  15. Pharmacist-directed Sputnik V (GAM-COVID-VAC) surveillance program: a prospective observational study in Southern India
  16. Correlation among Poincare plot and traditional heart rate variability indices in adults with different risk levels of metabolic syndrome: a cross-sectional approach from Southern India
  17. Does etodolac affect TRPA1 functionality in vivo in human?
  18. Dynamic of irisin secretion change after moderate-intensity chronic physical exercise on obese female
  19. Letter to the Editor
  20. The prospective record-breaking obesity drug tirzepatide raises concerns about affordability
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