Supplementation of antihypertensive drug regimen with vitamin E ameliorates alterations of primary haemodynamic parameters and total antioxidant capacity in ovariectomised rats
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Temitayo Olabisi Ajibade
,
Foluso B. Bolaji-Alabi
Abstract
Objectives
Ovariectomy induces heightened response to vasoconstrictors, alters vasorelaxation and consequently causes hypertension due to increased oxidative stress in rats.
Methods
This study evaluated the ameliorative effects of ramipril and vitamin E, on primary haemodynamic parameters and cardiac antioxidant defence status, in ovariectomised rats using 64 adult female rats of the Wistar strain randomly divided as follows: Control (sham); Ovariectomised (OVX); OVX plus Ramipril; OVX plus vitamin E; and OVX plus Ramipril plus vitamin E.
Results
The plasma level of oestrogen was significantly lower (p<0.05), in the ovariectomised rats compared with the sham. The systolic, diastolic and mean arterial blood pressure of ovariectomised rats increased significantly (p<0.05), but the alteration was significantly reduced by the administration of ramipril alone or in combination with vitamin E. Significant decrease (p<0.05) was observed in the serum level of nitric oxide in OVX group compared with Sham. Also, analysed markers of oxidative stress: Malondialdehyde (MDA) contents and hydrogen peroxide (H2O2) generated decreased significantly (p<0.05), but systemic antioxidants: reduced glutathione (GSH) contents; glutathione peroxidase (GPx) and superoxide dismutase (SOD) activities increased significantly (p<0.05) in the ovariectomised rats treated with ramipril and vitamin E compared with untreated ovariectomised rats. The study concludes that alteration, in the primary haemodynamic parameters, associated with ovariectomy in rats is potently ameliorated by co-administration of the antihypertensive drug ramipril and vitamin E.
Conclusions
The supplementation of antihypertensive regimen with antioxidants such as vitamin E in the treatment of hypertension is therefore justifiable especially in ovariectomised or hypogonadal patients.
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Research funding: The authors did not receive funding for the research.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Ethical approval: The University of Ibadan Animal Care Use and Research Ethics Committee (Ul-ACUREC) approved the study. Ethical approval number U.I. ACUREC/FBA/2018/004 was assigned.
References
1. 2Khajuria, D, Razdan, R, Mahapatra, D. Description of a new method of ovariectomy in female rats. Rev Bras Reumatol 2012;52:462–73. https://doi.org/10.1590/s0482-50042012000300016.Search in Google Scholar
2. Arenas, I, Armstrong, S, Xu, Y, Davidge, S. Tumor-necrosis factor-alpha and vascular angiotensin II in estrogen-deficient rats. Hypertension 2006;48:497–503. https://doi.org/10.1161/01.hyp.0000235865.03528.f1.Search in Google Scholar
3. Greendale, G, Derby, C, Maki, P. Perimenopause and cognition. Obstet Gynecol Clin N Am 2011;38:519–35. https://doi.org/10.1016/j.ogc.2011.05.007.Search in Google Scholar PubMed PubMed Central
4. Luo, T, Kim, JK. The role of estrogen and estrogen receptors on cardiomyocytes. Can J Cardiol 2016;32:1017–25. https://doi.org/10.1016/j.cjca.2015.10.021.Search in Google Scholar PubMed PubMed Central
5. Stice, J, Jason, P, Knowlton, E. Role of aging versus the loss of estrogens in the reduction in vascular function in female rats. Endocrinology 2009;150:212–19. https://doi.org/10.1210/en.2008-0640.Search in Google Scholar PubMed PubMed Central
6. Jordan, V. The new biology of estrogen-induced apoptosis applied to treat and prevent breast cancer. Endocr Relat Canc 2015;22:1–31. https://doi.org/10.1530/ERC-14-0448.Search in Google Scholar PubMed PubMed Central
7. Knowlton, A, Lee, A. Estrogen and the cardiovascular system. Pharmacol Ther 2012;135:54–70. https://doi.org/10.1016/j.pharmthera.2012.03.007.Search in Google Scholar PubMed PubMed Central
8. Safar, M, Blacher, J, Jankowski, P. Arterial stiffness, pulse pressure, and cardiovascular disease-is it possible to break the vicious circle? Atherosclerosis 2011;218:263–71. https://doi.org/10.1016/j.atherosclerosis.2011.04.039.Search in Google Scholar PubMed
9. Boshtam, M, Rafiei, M, Sadeghi, K, Sarraf-Zadegan, N. Vitamin E can reduce blood pressure in mild hypertensives. Int J Vitam Nutr Res 2002;72:309–14. https://doi.org/10.1024/0300-9831.72.5.309.Search in Google Scholar PubMed
10. Ward, N, Wu, J, Clarke, M, Puddey, I, Burke, V, Croft, K, et al.. The effect of vitamin E on blood pressure in individuals with type 2 diabetes: a randomized, double-blind, placebo-controlled trial. J Hypertens 2007;25:227–34. https://doi.org/10.1097/01.hjh.0000254373.96111.43.Search in Google Scholar PubMed
11. Andrew, R, Skyrme-Jones, P, O’Brien, R, Berry, K, Meredith, I. Vitamin E supplementation improves endothelial function in type I diabetes mellitus: a randomized, placebo-controlled study. J Am Coll Cardiol 2000;36:94–102. https://doi.org/10.1016/s0735-1097(00)00720-8.Search in Google Scholar PubMed
12. Förstermann, U. Nitric oxide and oxidative stress in vascular disease. Pflügers Archiv 2010;459:923–39. https://doi.org/10.1007/s00424-010-0808-2.Search in Google Scholar PubMed
13. Lasota, A, Danowska-Klonowska, D. Experimental osteoporosis – different methods of ovariectomy in female white rats. Rocz Akad Med Bialymst 2004;49:129–31.Search in Google Scholar
14. Gornal, A, Bardawill, J, David, M. Determination of serum proteins by means of biuret reaction. J Biol Chem 1949;177:751–66. https://doi.org/10.1016/s0021-9258(18)57021-6.Search in Google Scholar
15. Olaleye, S, Adaramoye, O, Erigbali, P, Adeniyi, O. Lead exposure increases oxidative stress in the gastric mucosa of HCl/ethanol-exposed rats. World J Gastroenterol 2007;3:5121–6. https://doi.org/10.3748/wjg.v13.i38.5121.Search in Google Scholar PubMed PubMed Central
16. Wolff, S. Ferrous ion oxidation in the presence of ferric ion indicator xylenol orange for measurement of hydrogen peroxides. Methods Enzymol 1994;233:182–9. https://doi.org/10.1016/s0076-6879(94)33021-2.Search in Google Scholar
17. Beutler, E, Duron, O, Kelly, B. Improved method for the determination of blood glutathione. J Lab Clin Med 1963;61:882–8.Search in Google Scholar
18. Misra, H, Fridovich, I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170–5. https://doi.org/10.1016/s0021-9258(19)45228-9.Search in Google Scholar
19. Habig, W, Pabst, M, Jacoby, W. Glutathione-S-transferase activity: the enzymic step in mercapturic acid formation. J Biol Chem 1974;249:130–9. https://doi.org/10.1016/s0021-9258(19)42083-8.Search in Google Scholar
20. Varshney, R, Kale, RK. Effect of calmodulin antagonists on radiation induced lipid peroxidation in microsomes. Int J Biol 1990;158:733–41. https://doi.org/10.1080/09553009014552121.Search in Google Scholar PubMed
21. Mercier, I, Pham-Dang, M, Clement, R, Gosselin, H, Colombo, F, Rouleau, JL, et al.. Elevated mean arterial pressure in the ovariectomized rat was normalized by ET(A) receptor antagonist therapy: absence of cardiac hypertrophy and fibrosis. Br J Pharmacol 2002;136:685–92. https://doi.org/10.1038/sj.bjp.0704765.Search in Google Scholar PubMed PubMed Central
22. Shen, H, Mei-Hui, L, Yu-Hui, C, Yen-Mei, L. Estrogen deficiency modifies matrix metalloproteinases activity and vascular function of mesenteric arteries in female rats. J Med Sci 2017;37:61–8.10.4103/jmedsci.jmedsci_100_16Search in Google Scholar
23. Pierini, D, Bryan, NS. Nitric oxide availability as a marker of oxidative stress. Methods Mol Biol 2015;1208:63–71. https://doi.org/10.1007/978-1-4939-1441-8_5.Search in Google Scholar PubMed
24. Lubos, E, Handy, D, Loscalzo, J. Role of oxidative stress and nitric oxide in atherothrombosis. Front Biosci 2008;13:5323–44. https://doi.org/10.2741/3084.Search in Google Scholar PubMed PubMed Central
25. Meister, A. Glutathione, ascorbate, and cellular protection. Cancer Res 1994;54:1969–75.Search in Google Scholar
26. Rowland, L, Shneider, N. Amyotrophic lateral sclerosis. N Engl J Med 2001;345:1131–2. https://doi.org/10.1056/nejm200110113451514.Search in Google Scholar
27. Hao, F, Ying, G, Xing, T. Estrogen replacement reduces oxidative stress in the rostral ventrolateral medulla of ovariectomized rats. Oxid Med Cell Longev 2016;2158971:1–8. https://doi.org/10.1155/2016/2158971.Search in Google Scholar PubMed PubMed Central
Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/jbcpp-2020-0097).
© 2021 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
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Articles in the same Issue
- Frontmatter
- Editorial
- Peyronie’s disease: where are we at?
- Reviews
- Catheter-associated urinary tract infection: an overview
- Liver and heart failure: an ultrasound relationship
- Original Articles
- The intracellular fluid compartment is smaller than commonly believed when measured by whole-body bioimpedance
- Prevalence and distribution of causes of infertility according to women’s age – a cross-sectional study in a tertiary healthcare hospital setup
- Supplementation of antihypertensive drug regimen with vitamin E ameliorates alterations of primary haemodynamic parameters and total antioxidant capacity in ovariectomised rats
- Analgesic effect of curcumin topical formulation in knee osteoarthritis patients: a clinical trial
- ProACT in the management of stress urinary incontinence after radical prostatectomy. What happens after 8 years of follow up? monocentric analysis in 42 patients
- The effect of metformin on the metabolism of human vascular smooth muscle cells in high glucose conditions
- Physiological response of endurance exercise as a growth hormone mediator in adolescent women’s
- Effect of Om chanting and Yoga Nidra on depression anxiety stress, sleep quality and autonomic functions of hypertensive subjects – a randomized controlled trial
- Effectiveness of electrical vestibular nerve stimulation as adjunctive therapy to improve the cognitive functions in patients with Parkinson’s disease
- Role of interleukin-22 in tuberculosis patients
- Effect of aspirin on the TNF-α-mediated cell survival and death pathways in breast cancer
- Resting heart rate variability as a diagnostic marker of cardiovascular dysautonomia in postural tachycardia syndrome
- Effects of intensity and training on cardiovascular responses to the Valsalva maneouvre
