The effect of metformin on the metabolism of human vascular smooth muscle cells in high glucose conditions
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Ali Akbar Soleimani
Abstract
Objectives
Metformin is widely used in type 2 diabetic patients as an antihyperglycemic drug. The aim of this study was to investigate the effect of metformin on the metabolism of vascular smooth muscle cells in high glucose conditions.
Materials and methods
The vascular smooth muscle cells were cultured in DMEM F12 containing glucose as high as 25 mM. The preconditioned cells were then treated with metformin in doses of 1, 5, and 7 mM for 24 h. MTT method was used to determine cell viability. Biochemical parameters including lactate, glucose, total protein, creatinine, and triglyceride were measured in the cell culture after the treatment with metformin. Oil Red O staining method was used to stain the lipids in the cells.
Results
Metformin reduced significantly (p<0.001) VSMC proliferation in a concentration-dependent manner. With the increase of glucose uptake by VSMCs, the cell lipid deposition was not changed. Other biochemical parameters such as lactate, triglyceride, total protein, and creatinine were significantly changed in the cell culture (p<0.05).
Conclusions
Metformin increased the glucose uptake impacting metabolic pathways in VSMCs. It also increased the lactate efflux and protein metabolism without the change in cellular lipid deposition in high glucose conditions.
Funding source: Iran University of Medical Sciences
Award Identifier / Grant number: 1400-1-4-19363
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Research funding: This study was supported by the Iran University of Medical Sciences (1400-1-4-19363).
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript.
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Competing interests: The authors did not have any conflicts of interest.
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Informed consent: No applicable.
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Ethical approval: The study was approved by the Ethics Committee of Iran University of Medical Sciences (IR.IUMS.FMD.REC.1400.182).
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Articles in the same Issue
- Frontmatter
- Editorial
- Peyronie’s disease: where are we at?
- Reviews
- Catheter-associated urinary tract infection: an overview
- Liver and heart failure: an ultrasound relationship
- Original Articles
- The intracellular fluid compartment is smaller than commonly believed when measured by whole-body bioimpedance
- Prevalence and distribution of causes of infertility according to women’s age – a cross-sectional study in a tertiary healthcare hospital setup
- Supplementation of antihypertensive drug regimen with vitamin E ameliorates alterations of primary haemodynamic parameters and total antioxidant capacity in ovariectomised rats
- Analgesic effect of curcumin topical formulation in knee osteoarthritis patients: a clinical trial
- ProACT in the management of stress urinary incontinence after radical prostatectomy. What happens after 8 years of follow up? monocentric analysis in 42 patients
- The effect of metformin on the metabolism of human vascular smooth muscle cells in high glucose conditions
- Physiological response of endurance exercise as a growth hormone mediator in adolescent women’s
- Effect of Om chanting and Yoga Nidra on depression anxiety stress, sleep quality and autonomic functions of hypertensive subjects – a randomized controlled trial
- Effectiveness of electrical vestibular nerve stimulation as adjunctive therapy to improve the cognitive functions in patients with Parkinson’s disease
- Role of interleukin-22 in tuberculosis patients
- Effect of aspirin on the TNF-α-mediated cell survival and death pathways in breast cancer
- Resting heart rate variability as a diagnostic marker of cardiovascular dysautonomia in postural tachycardia syndrome
- Effects of intensity and training on cardiovascular responses to the Valsalva maneouvre
