Evaluation of cytogenotoxic potential of Morinda lucida leaf extract on Swiss albino male mice using two bioassays
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Abass Toba Anifowoshe
,
Adam Olaitan Abdulkareem
Abstract
Background: Utilization of Morinda lucida for the treatment of ailments such as malaria, diarrhea, infertility in women, and dysentery in many countries including Nigeria is on increase due to its efficiency, availability, and affordability. However, its cytogenotoxicity has not been elucidated. This study investigated the phytochemical constituents and possible genotoxic and cytotoxic effects of M. lucida leaf extract in Swiss albino male mice using bone marrow micronucleus and sperm morphology assays. Methods: Plant materials was collected, thoroughly washed, and air-dried at room temperature prior to maceration. The extract was assessed for the presence of the phytochemical compounds. Swiss albino male mice (Mus musculus) (n=4) were treated with different concentrations of this extract (400 mg/kg, 800 mg/kg, and 1200 mg/kg) while distilled water was used as negative control. Results: Phytochemical analysis revealed that the extract contains saponins, terpenoids, flavonoids, glycosides, and tannins. We observed micronucleated polychromatic erythrocytes (PCE) and normochromatic erythrocytes (NCE) of 3.20%, 1.10%, and 1.95% at different concentrations of 400 mg/kg, 800 mg/kg, and 1200 mg/kg respectively in the treated animals. The result showed no significant increase in the frequency of abnormalities (p < 0.05) when compared to the negative control; however, in separate analysis of mono-micronucleated PCE and mono-micronucleated NCE per group, a significant increase at 400 mg/kg and 1200 mg/kg concentrations was observed. The sperm anomalies decreased with increase in concentration. Conclusions: This study recommends 800 mg/kg concentration of the plant extract; however, it should be further and properly investigated as it contains some pharmaceutical components that can be used for pharmacological purposes.
Acknowledgments
We acknowledge Mr Bolu (Taxonomist) of Department of Plant Biology, Faculty of Life Sciences, University of Ilorin, Ilorin, Nigeria, for his assistance in the identification of the plant.
Research funding: None declared.
Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
Competing interests: Authors state no conflict of interest.
Ethical approval: This study was carried out following strictly the guidelines on care and use of laboratory animals of the Ethical Committee of the University of Ilorin, Ilorin, Nigeria.
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Artikel in diesem Heft
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- Original Articles
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- Evaluation of cytogenotoxic potential of Morinda lucida leaf extract on Swiss albino male mice using two bioassays
- Acute and subacute toxicity evaluation of calcium carbide and ethylene glycol in Wistar albino rats
- Evaluation of the possible hepatotoxic and nephrotoxic potentials of the Averrhoa carambola juice extract in female albino rats
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- Prescribing pattern of antihypertensive medication and adherence to Joint National Commission-8 guidelines in a rural tertiary care Indian teaching hospital
- Assessment of cardiac risk in chronic asymptomatic alcoholics using blood pressure and electrocardiogram, and the relation with duration of drinking
- Effect of ghrelin on VEGF-B and connexin-43 in a rat model of doxorubicin-induced cardiomyopathy
- In vitro antioxidant and enzyme inhibitory properties of the n-butanol fraction of Senna podocarpa (Guill. and Perr.) leaf
- Blood pressure-reducing activity of Gongronema latifolium Benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry
- Evaluation of the anticonvulsant and anxiolytic-like activities of aqueous leaf extract of Cymbopogon citratus in mice
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- Miscellaneous
- Suspected reactivation of extrapulmonary tuberculosis focus after non-medical abuse of anabolic androgenic steroids: a case report
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Artikel in diesem Heft
- Minireview
- Pharmacokinetics and pharmacodynamics of mitragynine, the principle alkaloid of Mitragyna speciosa: present knowledge and future directions in perspective of pain
- Original Articles
- Trehalose protects against spinal cord injury through regulating heat shock proteins 27 and 70 and caspase-3 genes expression
- Evaluation of cytogenotoxic potential of Morinda lucida leaf extract on Swiss albino male mice using two bioassays
- Acute and subacute toxicity evaluation of calcium carbide and ethylene glycol in Wistar albino rats
- Evaluation of the possible hepatotoxic and nephrotoxic potentials of the Averrhoa carambola juice extract in female albino rats
- Chlorpyrifos and its metabolite modulates angiogenesis in the chorioallantoic membrane of chick embryo
- Prescribing pattern of antihypertensive medication and adherence to Joint National Commission-8 guidelines in a rural tertiary care Indian teaching hospital
- Assessment of cardiac risk in chronic asymptomatic alcoholics using blood pressure and electrocardiogram, and the relation with duration of drinking
- Effect of ghrelin on VEGF-B and connexin-43 in a rat model of doxorubicin-induced cardiomyopathy
- In vitro antioxidant and enzyme inhibitory properties of the n-butanol fraction of Senna podocarpa (Guill. and Perr.) leaf
- Blood pressure-reducing activity of Gongronema latifolium Benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry
- Evaluation of the anticonvulsant and anxiolytic-like activities of aqueous leaf extract of Cymbopogon citratus in mice
- Terpenoids and phytosteroids isolated from Commelina benghalensis Linn. with antioxidant activity
- Miscellaneous
- Suspected reactivation of extrapulmonary tuberculosis focus after non-medical abuse of anabolic androgenic steroids: a case report
- Acknowledgment
