Startseite Medizin Blood pressure-reducing activity of Gongronema latifolium Benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry
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Blood pressure-reducing activity of Gongronema latifolium Benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry

  • Justin Atiang Beshel , Javier Palacios , Favour Nyoh Beshel , Clement Oshie Nku , Daniel U. Owu EMAIL logo , Magdalene Nwokocha , Jorge Bórquez , Mario J. Simirgiotis und Chukwuemeka R. Nwokocha
Veröffentlicht/Copyright: 14. August 2019
Journal of Basic and Clinical Physiology and Pharmacology
Aus der Zeitschrift Journal of Basic and Clinical Physiology and Pharmacology Band 31 Heft 1

Abstract

Background

Gongronema latifolium Benth. (family Apocynaceae) leaves (GL) has interesting medicinal properties. The effects of extracts from G. latifolium on blood pressure (BP) and the possible mechanisms of action were also investigated.

Methods

The ultrahigh resolution liquid chromatography orbitrap MS analysis was used to identify the phytochemicals present. Normotensive Wistar rats were anesthetized with sodium pentobarbitone (40 mg/kg) intraperitoneally, and the jugular vein was cannulated for infusion of drugs while the carotid artery was cannulated for direct BP measurement. GL extract (5–20 mg) alone or with nifedipine (10 mg/kg), atropine (2 mg/kg), L-NAME (5 mg/kg), methyl blue (3 mg/kg) and propranolol (1 mg/kg) were administered intravenously to Wistar rats and direct BP measurements were carried out.

Results

Systolic and diastolic BP levels (128/90 mm Hg; MAP 103 ± 3 mm Hg) and heart rates were all significantly (p < 0.01) decreased after GL administration. Raised mean arterial pressure (MAP) and heart rate by atropine, L-NAME and methyl blue were significantly (p < 0.01) reduced after GL administration, while propranolol significantly (p < 0.01) inhibited hypotension caused by GL. Infusion of GL reduced MAP (95 ± 3 mm Hg) comparable with nifedipine (93 ± 2 mm Hg), a calcium channel blocker. The phytochemicals identified were 34 compounds, including oleanolic acid derivatives, flavonoids, antioxidant fatty acids, 2 coumarins and 2 iridoids.

Conclusions

These results suggest that G. latifolium has hypotensive properties mediated by the synergistic activity of the compounds, probably via the β-adrenergic blockade mechanism.

Keywords: Beta adrenergic receptor; blood pressure; calcium antagonism; Gongronema latifolium; mass spectrometry; phytochemicals

Funding source: FONDECYT

Award Identifier / Grant number: 1180059

Funding statement: Mario Simirgiotis and Jorge Bórquez acknowledge funds from FONDECYT, Funder Id: http://dx.doi.org/10.13039/501100002850, 1180059.

Acknowledgments

Dr. C. R. Nwokocha acknowledges funds from the University of The West Indies Mona Graduate School.

  1. Author contributions: All authors participated in the design, collection of data, interpretation of the studies, analysis of the data and review of the manuscript. JAB and FNB conducted the experiments; CON and DUO were involved in the design and data analysis; MN, JP, JB and MJS were involved in the UHPLC-MS analysis and interpretation; CRN carried out the data interpretation and drafting of the manuscript. All authors read and approved the final manuscript.

  2. Research funding: None declared.

  3. Employment or leadership: None declared.

  4. Honorarium: None declared.

  5. Competing interests: Authors state no conflict of interest.

  6. Ethical approval: Research involving animals complied with all relevant national regulations and institutional policies (Faculty of Basic Medical sciences, Animal Research Ethics Committee, University of Calabar) for the care and use of animals. (019PY20317).

References

[1] World Health Organization. A global brief on hypertension. WHO/DCO/WHD/2013.2, Switzerland.Suche in Google Scholar

[2] Unützer J, Klap R, Sturm R, Young A, Marmon T, Shatkin J, et al. Mental disorders and the use of alternative medicines: results from a national survey. Am J Psychiatry 2000;157:1851–7.10.1176/appi.ajp.157.11.1851Suche in Google Scholar

[3] Kadiri S, Walker O, Salako B, Akinkugbe O. Blood pressure, hypertension and correlates in urbanized workers in Ibadan, Nigeria: a revisit. J Hum Hypertens 1999;13:23–7.10.1038/sj.jhh.1000722Suche in Google Scholar

[4] Ajayi IO, Sowemimo IO, Akpa OM, Osai NE. Prevalence of hypertension and associated factors among residents of Ibadan-North Local Government Area of Nigeria. Niger J Cardiol 2016;13:67–75.10.4103/0189-7969.165168Suche in Google Scholar

[5] Ordinioha B, Brisibe S. Prevalence of hypertension and modifiable risk factors amongst traditional chiefs of an oil-bearing community in south-south Nigeria. Sahel Med J 2013;16:24–7.10.4103/1118-8561.112065Suche in Google Scholar

[6] Nwokocha CR, Owu DU, Kinlocke K, Murray J, Delgoda R, Thaxter K, et al. Possible mechanism of action of the hypotensive effect of Peperomia pellucida and interactions between human cytochrome P450 enzymes. Med Aromat Plants 2012;1:105.10.4172/2167-0412.1000105Suche in Google Scholar

[7] Abat JK, Kumar S, Mohanty A. Ethnomedicinal, phytochemical and ethnopharmacological aspects of four medicinal plants of Malvaceae used in Indian traditional medicines: a review. Medicines 2017;4:75.10.3390/medicines4040075Suche in Google Scholar

[8] Kumar A, Krishna G, Hullatti P, Akshara T. Indian plants with cardioprotective activity – a review. Sys Rev Pharmacol 2017;8:8.10.5530/srp.2017.1.3Suche in Google Scholar

[9] Ugochukwu NH, Babady NE. Antioxidant effects of Gongronema latifolium in hepatocytes of rat models of non-insulin dependent diabetes mellitus. Fitoterapia 2002;73:612–8.10.1016/S0367-326X(02)00218-6Suche in Google Scholar

[10] Hanelt P. Mansfeld´ encyclopedia of Agricultural and horticultural crops. Gatersleben, Germany: Springer, 2001.10.1007/978-3-540-30442-5Suche in Google Scholar

[11] Morebise O, Fafunso MA, Makinde JM, Olajide OA, Awe EO. Anti-inflammatory properties of the leaves of Gongronema latifolium. Phytother Res 2002;16:75–7.10.1002/ptr.784Suche in Google Scholar PubMed

[12] Ugochukwu NH, Babady NE, Cobourne MK, Gasset SR. The effect of Gongronema latifolium extract on serum lipid profile and oxidative stress in hepatocytes of diabetic rats. J Biosci 2003;28:1–5.10.1007/BF02970124Suche in Google Scholar

[13] Ugochukwu NH, Cobourne MK. Modification of renal oxidative stress and lipid peroxidation in streptozotocin-induced diabetic rats treated with extracts from Gongronema latifolium leaves. Clin Chim Acta 2003;336:73–81..10.1016/S0009-8981(03)00325-5Suche in Google Scholar

[14] Owu DU, Nwokocha CR, Obembe AO, Essien AD, Ikpi DE, Osim EE. Effect of Gongronema latifolium ethanol leaf extract on gastric acid secretion and cytoprotection in streptozotocin-induced diabetic rats. West Indian Med J 2012;61:853–60.10.7727/wimj.2011.040Suche in Google Scholar PubMed

[15] Antai AB, Ofem OE, Ikpi DE, Ukafia S, Agiang EA. Phytochemistry and some haematological changes following oral administration of ethanolic root extract of Gongronema latifolium in rats. Niger J Physiol Sci 2009;24:79–83.10.4314/njps.v24i1.46388Suche in Google Scholar

[16] Atangwho IJ, Ebong PE, Eyong EU, Williams IO, Eteng MU, Egbung GE. Comparative chemical composition of leaves of some antidiabetic medicinal plants. Afr J Biotechnol 2009;8:4685–9.Suche in Google Scholar

[17] Aziz S, Saha K, Sultana N, Nur HP, Ahsan MA, Ahmed S, et al. Comparative studies of elemental composition in leaves and flowers of Catharanthus roseus growing in Bangladesh. Asian Pac J Trop Biomed 2016;6:50–4.10.1016/j.apjtb.2015.10.003Suche in Google Scholar

[18] Eleyinmi AF. Chemical composition and antibacterial activity of Gongronema latifolium. J Zhejiang Univ Sci B 2007;8:352–8.10.1631/jzus.2007.B0352Suche in Google Scholar PubMed PubMed Central

[19] Simirgiotis MJ, Quispe C, Bórquez J, Schmeda-Hirschmann G, Avendaño M, Sepúlveda B, et al. Fast high resolution Orbitrap MS fingerprinting of the resin of Heliotropium taltalense Phil. from the Atacama Desert. Ind Crops Prod 2016;85:159–66.10.1016/j.indcrop.2016.02.054Suche in Google Scholar

[20] Mocan A, Moldovan C, Zengin G, Bender O, Locatelli M, Simirgiotis M, et al. UHPLC-QTOF-MS analysis of bioactive constituents from two Romanian Goji (Lycium barbarum L.) berries cultivars and their antioxidant, enzyme inhibitory, and real-time cytotoxicological evaluation. Food Chem Toxicol 2018;115:414–24.10.1016/j.fct.2018.01.054Suche in Google Scholar PubMed

[21] Sánchez-Montoya EL, Reyes MA, Pardo J, Nuñez-Alarcón J, Ortiz JG, Jorge JC, et al. High Resolution UHPLC447 MS Metabolomics and sedative-anxiolytic effects of Latua pubiflora: a mystic plant used by Mapuche Amerindians. Front Pharmacol 2017;8:494.10.3389/fphar.2017.00494Suche in Google Scholar PubMed PubMed Central

[22] Torres-Benítez A, Rivera-Montalvo M, Sepúlveda B, Castro O, Nagles E, Simirgiotis M, et al. Metabolomic analysis of two Parmotrema lichens: P. robustum (Degel.) Hale and P. andinum (Mull. Arg.) Hale using UHPLC-ESI-OT-MS-MS. Molecules 2017;22:1861.10.3390/molecules22111861Suche in Google Scholar PubMed PubMed Central

[23] Puangpraphant S, Berhow MA, de Mejia EG. Mate (Ilex paraguariensis St Hilaire) saponins induce caspase-3-dependent apoptosis in human colon cancer cells in vitro. Food Chem 2011;125:1171–8.10.1016/j.foodchem.2010.10.023Suche in Google Scholar

[24] Shan L, Wu Y, Yuan L, Zhang Y, Xu Y, Li Y. Rapid screening of chemical constituents in Rhizoma anemarrhenae by UPLC-Q-TOF/MS combined with data postprocessing techniques. Evidence-Based Compl Altern Med 2017; Article ID 4032820, 14 pages.10.1155/2017/4032820Suche in Google Scholar

[25] Lee MW, Hahn DR. Triterpenoidal saponins from the leaves of Kalopanax pictum var. chinense. Arch Pharmacol Res 199114:124–9.10.1007/BF02892016Suche in Google Scholar

[26] Shih YL, Au MK, Liu KL, Yeh MY, Lee CH, Lee MH, et al. Ouabain impairs cell migration, and invasion and alters gene expression of human osteosarcoma U-2 OS cells. Environ Toxicol 2017;32:2400–13.10.1002/tox.22453Suche in Google Scholar

[27] de Souza LM, Dartora N, Scoparo CT, Cipriani TR, Gorin PA, Iacomini M, et al. Comprehensive analysis of mate (Ilex paraguariensis) compounds: development of chemical strategies for matesaponin analysis by mass spectrometry. J Chromatogr 2011;1218:7307–15.10.1016/j.chroma.2011.08.047Suche in Google Scholar

[28] Brito A, Areche C, Sepulveda B, Kennelly EJ, Simirgiotis MJ. Anthocyanin characterization, total phenolic quantification and antioxidant features of some chilean edible berry extracts. Molecules 2014;19:10936–55.10.3390/molecules190810936Suche in Google Scholar

[29] Jiang B, Zhang ZW. Comparison on phenolic compounds and antioxidant properties of Cabernet sauvignon and merlot wines from four wine grape-growing regions in China. Molecules 2012;17:8804–21.10.3390/molecules17088804Suche in Google Scholar

[30] Spiteller P, Spiteller G. 9-Hydroxy-10,12-octadecadienoic acid (9-HODE) and 13-hydroxy-9,11-octadecadienoic acid (13-HODE): excellent markers for lipid peroxidation. Chem Phys Lipids 1997;89:131–9.10.1016/S0009-3084(97)00070-4Suche in Google Scholar

[31] Simirgiotis MJ, Ramirez JE, Hirschmann GS, Kennelly EJ. Bioactive coumarins and HPLC-PDA-ESI-ToF-MS metabolic profiling of edible queule fruits (Gomortega keule), an endangered endemic Chilean species. Food Res Int 2013;54:532–43.10.1016/j.foodres.2013.07.022Suche in Google Scholar

[32] Jerkovic I, Tuberoso CI, Marijanovic Z, Kranjac M, Malenica-Staver M. Antioxidant capacity and chemical profiles of Jerkovic I, Tuberoso CI, Marijanovic Z, Kranjac M, Malenica-Staver M. Antioxidant capacity and chemical profiles of Satureja montana l. honey: hotrienol and syringyl derivatives as biomarkers. Chem Biodivers 2015;12:1047–56.10.1002/cbdv.201400183Suche in Google Scholar PubMed

[33] Egbewande FA, Nilsson N, White JM, Coster MJ, Davis RA. The design, synthesis, and anti-inflammatory evaluation of a drug-like library based on the natural product valerenic. Bioorg Med Chem Lett 2017;27:3185–9.10.1016/j.bmcl.2017.05.021Suche in Google Scholar PubMed

[34] Alipieva K, Simova S, Zahmanov G, Zhou S, Wolfender JL, Georgiev MI. New tetraacetylated iridoid glycosides from Sambucus ebulus L. leaves. Phytochem Lett 2017;20:429–32.10.1016/j.phytol.2017.01.003Suche in Google Scholar

[35] Nwokocha CR, Owu DU, Gordon A, Thaxter K, McCalla G, Ozolua RI, et al. Possible mechanisms of action of the hypotensive effect of Annona muricata (soursop) in normotensive Sprague-Dawley rats. Pharm Biol 2012;50:1436–41.10.3109/13880209.2012.684690Suche in Google Scholar PubMed

[36] Adeneye AA, Ajagbonna OP, Mojiminiyi FB, Odigie IP, Ojobor PD, Etarrh RR. The hypotensive mechanisms for the aqueous stem bark extract of Musanga cercropioides in Sprague-Dawley rats. J Ethnopharmacol 2006;106:203–7.10.1016/j.jep.2005.12.023Suche in Google Scholar PubMed

[37] Tom EN, Demougeot C, Mtopi OB, Dimo T, Djomeni PD, Bilanda DC, et al. The aqueous extract of Terminalia superba (Combretaceae) prevents glucose-induced hypertension in rats. J Ethnopharmacol 2011;133:828–33.10.1016/j.jep.2010.11.016Suche in Google Scholar PubMed

[38] Ahmad M, Gilani AU, Aftab K, Ahmad VU. Effects of kaempferol-3-O-rutinoside on rat blood pressure. Phytother Res 1993;7:314–6.10.1002/ptr.2650070411Suche in Google Scholar

[39] Jin Y, Tang YP. Pharmacokinetic comparison of seven major bio-active components in normal and blood stasis rats after oral administration of herb pair Danggui-Honghua by UPLC-TQ/MS. Molecules 2017;22:1746.10.3390/molecules22101746Suche in Google Scholar PubMed PubMed Central

[40] Nwokocha C, Palacios J, Simirgiotis MJ, Thomas J, Nwokocha M, Young L, et al. Aqueous extract from leaf of Artocarpus altilis provides cardio-protection from isoproterenol induced myocardial damage in rats: negative chronotropic and inotropic effects. J Ethnopharmacol 2017;203:163–70.10.1016/j.jep.2017.03.037Suche in Google Scholar PubMed

[41] Najmanová I, Doseděl M, Hrdina R, Anzenbacher P, Filipský T, Říha M, et al. Cardiovascular effects of coumarins besides their antioxidant activity. Curr Top Med Chem 2015;15:830–49.10.2174/1568026615666150220112437Suche in Google Scholar PubMed

[42] Hodgson JM, Croft KD. Dietary flavonoids: effects on endothelial function and blood pressure. J Food Agric 2006;86:1097–210.10.1002/jsfa.2675Suche in Google Scholar

[43] Ajay M, Chai HJ, Mustafa AM, Gilani AH, Mustafa MR. Mechanisms of the antihypertensive effect of Hibiscus sabdariffa L. calyses. J Ethnopharmacol 2007;109:388–93.10.1016/j.jep.2006.08.005Suche in Google Scholar PubMed

[44] Güçlü-Üstündağ O, Mazza G. Saponins: properties, applications and processing. Critical Rev Food Sci Nutri 2007;47:231–58.10.1080/10408390600698197Suche in Google Scholar PubMed

[45] Li W, Yan XT, Sun YN, Ngan TT, Shim SH, Kim YH. Anti-inflammatory and PPAR transactivational effects of oleanane-type triterpenoid saponins from the roots of Pulsatilla koreana. Biomol Ther (Seoul) 2014;22:334–40.10.4062/biomolther.2014.047Suche in Google Scholar PubMed PubMed Central

[46] Kim DS, Goo YM, Cho J, Lee J, Lee DY, Sin SM, et al. Effect of volatile organic chemicals in Chrysanthemum indicum Linné on blood pressure and electroencephalogram. Molecules 2018;23:2063.10.3390/molecules23082063Suche in Google Scholar PubMed PubMed Central

[47] Naseem R, Adam AM, Khan F, Dossal A, Khan I, Khan A, et al. Prevalence and characteristics of resistant hypertensive patients in an Asian population. Indian Heart J 2017;69:442–6.10.1016/j.ihj.2017.01.012Suche in Google Scholar PubMed PubMed Central

Supplementary Material

The online version of this article offers Supplementary material (DOI: 10.1515/jbcpp-2018-0178).

Received: 2018-10-03
Accepted: 2019-06-25
Published Online: 2019-08-14

© 2020 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/jbcpp-2018-0178
Schlagwörter für diesen Artikel
Beta adrenergic receptor; blood pressure; calcium antagonism; Gongronema latifolium; mass spectrometry; phytochemicals

Artikel in diesem Heft

  1. Minireview
  2. Pharmacokinetics and pharmacodynamics of mitragynine, the principle alkaloid of Mitragyna speciosa: present knowledge and future directions in perspective of pain
  3. Original Articles
  4. Trehalose protects against spinal cord injury through regulating heat shock proteins 27 and 70 and caspase-3 genes expression
  5. Evaluation of cytogenotoxic potential of Morinda lucida leaf extract on Swiss albino male mice using two bioassays
  6. Acute and subacute toxicity evaluation of calcium carbide and ethylene glycol in Wistar albino rats
  7. Evaluation of the possible hepatotoxic and nephrotoxic potentials of the Averrhoa carambola juice extract in female albino rats
  8. Chlorpyrifos and its metabolite modulates angiogenesis in the chorioallantoic membrane of chick embryo
  9. Prescribing pattern of antihypertensive medication and adherence to Joint National Commission-8 guidelines in a rural tertiary care Indian teaching hospital
  10. Assessment of cardiac risk in chronic asymptomatic alcoholics using blood pressure and electrocardiogram, and the relation with duration of drinking
  11. Effect of ghrelin on VEGF-B and connexin-43 in a rat model of doxorubicin-induced cardiomyopathy
  12. In vitro antioxidant and enzyme inhibitory properties of the n-butanol fraction of Senna podocarpa (Guill. and Perr.) leaf
  13. Blood pressure-reducing activity of Gongronema latifolium Benth. (Apocynaeceae) and the identification of its main phytochemicals by UHPLC Q-Orbitrap mass spectrometry
  14. Evaluation of the anticonvulsant and anxiolytic-like activities of aqueous leaf extract of Cymbopogon citratus in mice
  15. Terpenoids and phytosteroids isolated from Commelina benghalensis Linn. with antioxidant activity
  16. Miscellaneous
  17. Suspected reactivation of extrapulmonary tuberculosis focus after non-medical abuse of anabolic androgenic steroids: a case report
  18. Acknowledgment
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