Antioxidant potentials and effects on the hematology and osmotic fragility scores of a polyherbal formulation used in Southeast Nigeria
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Solomon Nnah Ijioma
, Eme Eme Osim
Abstract
Background
In this study, the hematological and antioxidant potential as well as the osmotic fragility effects of a Nigerian polyherbal formulation were evaluated.
Materials and methods
A total of 40 fats were divided into four groups of 10 rats each. Group 1 served as the control group, and the rest were assigned increasing daily oral administration of the extract for 28 days. At the end of treatment, blood was collected for hematological and osmotic fragility studies. The free radical scavenging effect of the extract was investigated via different in vitro models as well.
Results
Results showed that the nitric oxide scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) activities of the extract were significant (p < 0.05) and compared favorably with that of vitamin C. At 200 and 400 μg/mL, the nitric oxide scavenging activities for Ajumbise Polyherbal Extract (APE) were 60.71 ± 0.25% and 59.49 ± 0.98%, respectively, whereas for the same concentrations of vitamin C, 74.60 ± 0.25% and 85.24 ± 0.14 scavenging activities were obtained. The (DPPH) activity at 100 μg/mL was 81.24 ± 0.02% for the extract and 96.22 ± 0.18% for vitamin C. However, at all concentrations, the extract had significantly lower Ferric Reducing Antioxidant Power (FRAP) activity than vitamin C. Red blood cell counts (RBCC), hemoglobin and packed cell volume values (PCV) were significantly lowered only in groups treated with 400 and 800 mg/kg of the extract (p < 0.05), whereas other RBCC parameters and white blood cell counts (WBCC) were not significantly affected (p < 0.05). Platelet (PLT) count was also significantly lowered in all extract-treated groups. The extract also significantly reduced RBCC percentage hemolysis (p < 0.05).
Conclusions
Ajumbise polyherbal may be free of hematoxicity and may improve the integrity of the RBC membrane due to its appreciable antioxidant activity.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Competing interests: Authors state no potential conflict of interest.
Ethical approval: The research related to animals’ use has been complied with all the relevant national regulations and institutional policies (as approved by the Physiology Laboratory of the Department of Physiology and Pharmacology, College of Veterinary Medicine, Michael Okpara University of Agriculture, Umudike, Nigeria) for the care and use of animals. (National Institutes of Health - NIH, 2011).
References
[1] Ijioma SN, Okafor AI, Ndukuba PI, Akomas SC. Hypoglycemic, Haematologic and Hypolipidemic activity of Jatropha tanjorensis ethanol leaf extract in alloxan induced diabetic rats. Ann Biol Res. 2014;5:15–19.Search in Google Scholar
[2] Devasagayam TP, Tilak JC, Tilak KK, Boloor KS, Saroj SG, Lele RD. Free radicals and antioxidants in human health: current status and future prospects. J Assoc Phys India 2004;9:52.Search in Google Scholar
[3] Dragan AD, Davidovi A, Drago B, Nenad T. Structure-radical scavenging activity relationships of flavonoids. Croatica Chemica Acta 2003;76:55–61.Search in Google Scholar
[4] Hamzah RU, Jigam AA, Makun HA, Egwim EC. Antioxidant properties of selected african vegetables, fruits and mushrooms: a review. 2006. DOI: 10.5772/52771.Search in Google Scholar
[5] Emeka EJ, Fang FL, Rang RC, Yan L, Conrad AO, Ying JZ. Anti-cancer and free radical scavenging activity of some Nigerian food plants in vitro. Int J Cancer Resb 2015;11:41–51.10.3923/ijcr.2015.41.51Search in Google Scholar
[6] Mau JL, Lin HC, Song SF. Antioxidant properties of several specialty mushrooms. Food Res Int 2002;35:519–26.10.1016/S0963-9969(01)00150-8Search in Google Scholar
[7] Farrukh A, Iqbal A, Zafar M. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk J Biol 2006;30:177–83.Search in Google Scholar
[8] Adenkola AY, Oluremi OI. Erythrocyte osmotic fragility score in rabbits fed hibiscus sabdariffa in graded level. Niger J Physiol Sci 2014;29:113–7.Search in Google Scholar
[9] Odukoya OA, Inya-Agha SI, Segun FI, Sofidiya OO, Ilori OO. Antioxidant activitry of selected Nigerian green leafy vegetables. Am J Food Tech 2007;2:167–75.10.3923/ajft.2007.169.175Search in Google Scholar
[10] Edet EC, Ofem JE, Igile GO, Ofem OE, Zainab DB, Akwaowo G. Antioxidant capacity of different African seeds and vegetables and correlation with the contents of ascorbic, phenolics and flavonoids. J Med Plant Res 2015;9:456–61.10.5897/JMPR2014.5660Search in Google Scholar
[11] Mpiana PT, Ngbolua KN, Bokota MT, Kasonga TK, Atibu EK, Tshibangu DS, et al. In vitro effects of anthocyanin extracts from Justicia secunda Vahl on the solubility of haemoglobin S and membrane stability of sickle erythrocytes. Blood Transfus 2010;8:248–54.Search in Google Scholar
[12] Pacini N, Ferrari A, Menozzi M, Borziani F. Melatonin enhances the in vitro action of cytochalasin B on globular resistance and osmotic fragility of erythrocytes. Neuro Endocrinol Lett 2011;32:292–300.Search in Google Scholar
[13] Ijioma SN, Osim EE, Nwankwo AA, Nwosu CO, Nwankudu ON, Igwe KK, et al. Plants composition and identification of phytocomponents in a polyherbal formulation used in southeast Nigeria. CARD Int J Med Appl Biosci 2017;1:84–98.Search in Google Scholar
[14] Yao LH, Jang YM, Shi J, Tomas-Barberan FA, Datta N, Singanu-Song R, et al. Flavonoids in food and their health benefits. Plants Foods Human Nutr 2004;59:113–22.10.1007/s11130-004-0049-7Search in Google Scholar
[15] Lewis D, Field W and Shaw G. A natural flavonoid present in unripe plankain banana pulp protects the gastric mucosa from aspirin-induced erosion. J Ethnorpharm 1999;65:283–6.10.1016/S0378-8741(99)00005-7Search in Google Scholar
[16] Kumar M, Manish KG, Anit S, Goel RJ. Healing effects of Musa sapientum var. Paradisiacal in diabetic rats with co-occuring gastric ulcer, cytokines and growth factor by PCR amplification. BML Complement Altern Med 2013;13:305.10.1186/1472-6882-13-305Search in Google Scholar PubMed PubMed Central
[17] Chung KT, Wong TY, Wei CT. Tannins and human health: a review. Crit Rev Food Sci 1998;38:421–34.10.1080/10408699891274273Search in Google Scholar PubMed
[18] Huang Q, Lu G, Shan HM, Chung MC, Ong CN. Anticancer properties of anthraquinones from rhubarb. Med Res Rev 2007;27:609–30.10.1002/chin.200747265Search in Google Scholar
[19] OECD. Guidelines for the Testing of Chemicals/Section 4: Health Effects Tests no, 423: Acute oral Toxicity – Acute Toxic Class method. Paris: Organization for Economic Cooperation and Development, 2001.Search in Google Scholar
[20] Adenkola AY, Oluremi OI. Erythrocyte osmotic fragility score in rabbits fed Hibiscus sabdariffa in graded level. Niger J Physiol Sci 2014;29:113–7.Search in Google Scholar
[21] Marcocci JJ, Maguire MT, Droylefaix LP. The nitric oxide-scavenging properties of ginkgo biloba extract EGb 761. Biochem Biophys Res Commun 1994;201:748–55.10.1006/bbrc.1994.1764Search in Google Scholar PubMed
[22] Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem 1982;126:131–8.10.1016/0003-2697(82)90118-XSearch in Google Scholar
[23] Mensor LL, Fabio SM, Gilda GL, Alexandre SR, Tereza CD, Cintia SC, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 2001;15:127–30.10.1002/ptr.687Search in Google Scholar
[24] Iwalewa EO, Adewale IO, Aiwo BJ, Arogundabe T, Osinowo A, Daniyan OM, et al. Effects of Harungana madagascariensis stem bark extract on the antiodixant markers in alloxan induced diabetic and carrageenan induced inflammatory disorders in rats. J Comp Int Med 2008;5:1–18.10.2202/1553-3840.1088Search in Google Scholar
[25] Benzie FF, Strain JJ. Ferric reducing/antioxidant power Assay: direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;299:15–23.10.1016/S0076-6879(99)99005-5Search in Google Scholar
[26] Chernecky CC, Krech RL, Berger BJ. Laboratory tests and diagnostic procedures. Philadelphia: W.B. Saunders Company, a Division of Harcourt Brace & Company, 1993;252–255:638–9.Search in Google Scholar
[27] Horn RC, Vagas VF. Antimutagenic activity of extracts of natural substances in the Salmonella/microsome assay. Oxford J (mutagenesis) 2002;18:113–8.10.1093/mutage/18.2.113Search in Google Scholar
[28] Saliu JA, Elekofehinti OO, Komolafe K, Oboh G. Effects of some green leafy vegetables on the haematological parameters of diabetic rats. J Nat Prod Plant Res 2012;2:482–5.Search in Google Scholar
[29] Orhue ES, Idu M, Ataman JE, Ebite LE. Haematological and histopathological studies of Jatropha tanjorensis leaves in Rabbits. Asian J Bio Sci 2008;1:84–9.10.3923/ajbs.2008.84.89Search in Google Scholar
[30] Ita SO, Udofia UA. Comparative stydu of some haematological parameters in rat following ingestion of crude oil (Nigerian Bony light), petrol, kerosene and diesel. Asian J Bio Sci 2011;4:498–505.10.3923/ajbs.2011.498.505Search in Google Scholar
[31] Akomas SC, Ijioma SN. Bleeding and clotting time effects of ethanolic extracts of Chromolaena odorata versus Ocimum gratissimum treated albino rats. Com J Med Sci 2014;2:9–13.Search in Google Scholar
[32] Torres-Urrutia C, Guzman L, Schmeda-Hirschman G, Moore-Carrasco R, Alavcon M, Astuillo L, et al. Anti-platelet, anti-coagulant and fibrinolytic activity in vitro of extracts from selected fruits and vegetables. Blood Coagul Fibrinolysis 2011;22:197–205.10.1097/MBC.0b013e328343f7daSearch in Google Scholar
[33] Inyang OI, Okon UA, Ekpeyong U. Reduction of platelets and lymphocytes counts and elevation of neutrophil counts in rats treated with aqueous leaf extract of Ocimum gratissimum. Afri J Biochem Res 2011;5:22–8.Search in Google Scholar
[34] Guyton AC, Hall JE. Textbook of medical physiology. Philadelphia: W.B Saunders Company, 1996:771–8.Search in Google Scholar
[35] Ashafa AO, Grierson DS, Afolayan AJ. In vitro antioxidant activity of extracts from Felicia muricata thumb: An under-utilized medicinal plant in the eastern cape province South Africa. Afri J Trad Comp Alt Med 2010;7:294–302.10.4314/ajtcam.v7i4.56695Search in Google Scholar
[36] Sindhu T, Rajamanikandan S, Srinivasan P. In vitro antioxidant and anti-bacterial activities of methanol extract of Kylinga nemoralis. Ind J Pharm Sci 2014;76:170–4.Search in Google Scholar
[37] Nayan RB, Pankaj BN, Acharya RN, Shukia VJ. In vitro antioxidant activity of hydro-alcoholic extract from the fruit pulp of Cassia fistula Linn. Ayu 2013;34:209–14.10.4103/0974-8520.119684Search in Google Scholar
[38] Suprava S, Goutam G, Debaju-Oti D, Sangharitra N. Phytochemical investigation and in vitro antioxidant activity of an indigenous medicinal plant Alpinia nigra. Asian Pac J Trop Biomed 2013;3:871–6.10.1016/S2221-1691(13)60171-9Search in Google Scholar
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Articles in the same Issue
- Minireview
- Glial pathology in neuropsychiatric disorders: a brief review
- Original Articles
- Fenugreek seed extract ameliorates cognitive deficits in streptozotocin-induced diabetic rats
- The effect of silymarin supplementation on cognitive impairment induced by diabetes in rats
- Evaluation of the antianxiety and antidepressant activities of mosapride in Wistar albino rats
- Sexual stimulant effects of the mixture of Mondia whitei, Dracaena arborea, and Bridelia ferruginea in normal and prediabetic male Wistar rats
- Evaluating exercise challenge to validate cardiac autonomic dysfunction in lean PCOS phenotype
- Involvement of proinflammatory cytokines and metallothionein in the repairing of arsenic-mediated uterine tissue damage by curcumin
- Antioxidant potentials and effects on the hematology and osmotic fragility scores of a polyherbal formulation used in Southeast Nigeria
- Protective mechanisms of protocatechuic acid against doxorubicin-induced nephrotoxicity in rat model
- Phytochemicals and antidiabetic activity of the aqueous extract of the Punica granatum fruit in streptozotocin-induced diabetic mice
- Ficus cunia Buch.-Ham. ex Roxb. (leaves): An experimental evaluation of the cytotoxicity, thrombolytic, analgesic and neuropharmacological activities of its methanol extract
- Ameliorative effects of Annona muricata Linn. (Annonaceae) against potassium dichromate-induced hypertension in vivo: involvement of Kim-1/p38 MAPK/Nrf2 signaling
- Case Report
- A rare case of clomiphene-induced leukocytoclastic vasculitis