Antidiarrheal and antimicrobial activities of the ethanol extract from the Icacina senegalensis root bark
-
Godwin C. Akuodor
,
Ndubuisi N. Nwobodo
Abstract
Background:
The Icacina senegalensis root bark is traditionally used for the treatment of gastrointestinal disorders in Nigeria. To date, no scientific study has substantiated or refuted this claim.
Methods:
The antidiarrheal and antimicrobial activities of the ethanol root bark extract were investigated in rats and against some selected diarrhea-causing microorganisms.
Results:
The extract significantly decreased the frequency of castor oil-induced diarrhea, and inhibited the masses and volumes of intestinal fluid accumulation in the castor oil-induced enteropooling method. The distance travelled by the charcoal meal was also decreased by the extract gastrointestinal transit method. The extract also strongly inhibited the growth of some selected microorganisms.
Conclusions:
The ethanol extract of the I. senegalensis root bark showed antidiarrheal activity, thus justifying its long folkloric use in diarrhea treatment. The extract also demonstrated antimicrobial activity against selected diarrheal causing organisms.
Acknowledgments
The authors acknowledge the support of TETFUND in funding the study. The authors also wish to thank Mr. Simon Eze Nwibo and Mr. Chibueze Nwonu for their technical assistance.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved its submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Omoboyowa DA, Fred O, Nwodo C, Elijah P. Anti-diarrhoeal activity of Chloroform-ethanol extracts of Cashew (Anacardium occidentale) kernel. J Nat Prod 2013;6:109–17.Search in Google Scholar
2. Idowu OA, Soniran OT, Ajana O, Aworinde DO. Ethnobotanical survey of anti-malarial plants used in Ogun State, Southwest Nigeria. Afr J Pharm Pharmacol 2009;4:55–60.Search in Google Scholar
3. Srivastava J, Lambert J, Vietmeyer N. Medicinal plants: an expanding role in development. World Bank Technical 1996; Paper. No. 320. 20.7.10.1596/0-8213-3613-4Search in Google Scholar
4. Sarr OS, Perrotey S, Fall I, Ennahar S, Zhao M, Diop Y, et al. Icacina senegalensis (Icacicaceae), traditionally used for the treatment of malaria, inhibits in vitro Plasmodium falciparum growth without host cell toxicity. Malar J 2011;10:85.10.1186/1475-2875-10-85Search in Google Scholar
5. Essien David-Oku, Obiajunwa-Otteh JI, Akuodor GC, Essien AD. Evaluation of the antimalarial potential of Icacina senegalensis Juss (Icacinaceae). Asian Pac J Trop Med 2014;7:S469–72.10.1016/S1995-7645(14)60276-5Search in Google Scholar
6. Mbatchou VC, Dawda S. Phytochemical and pharmacological profile of genus Icacina. Phytopharmacol 2012;2:135–43.Search in Google Scholar
7. Obiajunwa-Otteh JI, Akuodor GC, Onyekpe PI, Umoh EU. Phytochemical and antimicrobial potency of aqueous and methanol leaf Extracts of Icacina senegalensis Pharmacologia 2014;5:321–5.10.5567/pharmacologia.2014.321.325Search in Google Scholar
8. Akuodor GC, Udia PM, Bassey A, Chilaka KC, Okezie OA. Antihyperglycemic and antihyperlipidemic properties of aqueous root extract of Icacina senegalensis in alloxan induced diabetic rats. J Acute Disease 2014;3:99–103.10.1016/S2221-6189(14)60025-1Search in Google Scholar
9. David-Oku E, Akuodor GC, Ogbuji GK, Edet EE, Obiajunwa-Otteh JI, Aja DO. Antinociceptive, anti-inflammatory and antipyretic effects of ethanolic root bark extract of Icacina senegalensis in rodents. J Appl Pharm Sci 2016;6:104–8.10.7324/JAPS.2016.60215Search in Google Scholar
10. Akuodor GC, David-Oku E, Nkorroh JA, Essien AD, Nkanor EE, Ezeunala MN, et al. Antiplasmodial activity of the ethanolic root bark extract of Icacina senegalensis in mice infected by Plasmodium berghei. J Basic Clin Physiol Pharmacol 2017;28:181–4.10.1515/jbcpp-2016-0109Search in Google Scholar PubMed
11. Burkill HM. The useful plants of west tropical Africa, 2nd ed. Vol. 3. Kew (London): Royal Botanical Garden, 1985.Search in Google Scholar
12. Kuroyanagi T. Revision to the WMA Declaration of Helsinki. Japan Med Assoc J 2009;52:293–318.Search in Google Scholar
13. National Institute of Health (NIH). Committee for the update of the guide for the Care and use of laboratory animals. Washington (DC): National Academies Press (US), 2011.Search in Google Scholar
14. Oloyede OI. Chemical profile of unripe pulp of Carica papaya. Pak J Nutr 2005;4:379–81.10.3923/pjn.2005.379.381Search in Google Scholar
15. Mukherjee PR. Quality control of herbal drugs, an approach to evaluation of botanicals, 13th ed. New Delhi: Business Horizones Publishers, 2006:419–49.Search in Google Scholar
16. Ajayi AO. Antimicrobial nature and use of some medicinal plants in Nigeria. Afr J Biotechnol 2008;7:595–9.Search in Google Scholar
17. Organisation for Economic co-operation and Development (OECD). Guidelines for the testing of chemicals. Testing number 423 acute oral toxicity. Acute Toxicity Class Method 2010;1:1–14.Search in Google Scholar
18. Sunil B, Bedi K, Singla A, Johri R. Antidiarrhoeal activity of piperine in mice. Planta Medica 2001;67:284–7.10.1055/s-2001-11999Search in Google Scholar PubMed
19. Qnais EY, Elokda AS, Ghalyun YA, Abdulla FA. Antidiarrhea activity of the aqueous extract of Punica granatum (Pomegranate) peels. Pharm Biol 2007;45:715–20.10.1080/13880200701575304Search in Google Scholar
20. Gerald NT, Jules RK, Omer BN, Donatie G. Antidiarrhoeal and antimicrobial activities of Emilia coccinea (Sims) G. Don extracts. J Ethnopharmacol 2007;112:278–83.10.1016/j.jep.2007.03.007Search in Google Scholar
21. Akuodor GC, Idris-Usman M, Ugwu TC, Akpan JL, Irogbeyi LA, Iwuanyanwu TC, et al. Ethanolic leaf extract of Verbena hastata produces antidiarrhoeal and gastrointestinal slowing effects in albino rats. J Med Plants Res 2010;4:1624–7.Search in Google Scholar
22. Oben JE, Assi SE, Agbor GA, Musoro DF. Effect of Eremomastax speciosa on experimental diarrhoea. Afr J Trad Compl Altern Med 2006;3:95–100.10.4314/ajtcam.v3i1.31144Search in Google Scholar
23. Havagiray R, Ramesh C, Sadhna K. Study of antidiarrhoeal activity of Calotropis gigantean in experimental animals. J Pharm Sci 2004;7:70–5.Search in Google Scholar
24. Akanji MA, Yakubu MT. α-Tocopherol protects against metabisulphite- induced tissue damage in rats. Nigerian J Biochem Mol Biol 2000;15:179–83.Search in Google Scholar
25. Akuodor GC, Onyewenjo SC, Anyalewechi NA, Essien AD, Akpan JL, Okoroafor DO, et al. In vitro antimicrobial activity of leaf extract of Berlina grandiflora Huth. and Dalz. Afr J Microbiol Res 2011;5:1358–60.10.5897/AJMR10.692Search in Google Scholar
26. Adebayo EA, Ishola OR. Phytochemical and antimicrobial screening of Crude extracts from the root, stem bark and leaves of Terminalia Glaucescens. Afr J Pharm Pharmacol 2009;3: 217–21.Search in Google Scholar
27. Andrews JM. Determination of minimum inhibitory concentration. J Antimicrob Chemother 2001;48:5–16.10.1093/jac/48.suppl_1.5Search in Google Scholar
28. Dunnett C, Goldsmith C. In: Buncher CR, Tsay JY, editors. Statistics in the pharmaceutical industry, 2nd ed. NY: Marcel Dekker, 1993;397–433.Search in Google Scholar
29. Ganginella TS, Steward JJ, Oslo WA, Bass P. Actions of ricinoleic acid and structurally related fatty acid on the gastrointestinal tract11. Effect on water and electrolyte absorption in vitro. J Pharmacol Exp Ther 1975;195:355–61.Search in Google Scholar
30. Tunaru S, Althoff TF, Nusing RM, Diener M, Offermanns S. Castor oil induces laxation and uterus contraction via ricinoleic acid activating prostaglandin EP3 receptor. Proc Natl Acad Sci USA 2012;109:9179–84.10.1073/pnas.1201627109Search in Google Scholar
31. Zavala MA, Perez S, Perez ZC, Vergas B, Perez RM. Anti-diarrhoeal activity of Walfheria Americana, Commelina coelestis and Alternanthera repeus. J Ethnopharmacol 1998;61:41–7.10.1016/S0378-8741(98)00014-2Search in Google Scholar
32. Izzo AA, Mascolo N, Capasso R, Germano MP, Depasquel R, Capasso F. Inhibitory effect of cannabinoid agonist on gastric emptying in the rat. Arch Pharmacol 1999;360:221–3.10.1007/s002109900054Search in Google Scholar PubMed
33. Brijesh S, Daswani P, Tetali P, Antia N, Birdi T. Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: validating its traditional usage. BMC Comp Altern Med 2009;9:1–12.10.1186/1472-6882-9-47Search in Google Scholar PubMed PubMed Central
34. Saralaya MG, Patel P, Patel M, Roy SP, Patel AN. Anti-diarrhoeal activity of methanolic extract of Moringa oleifera lam roots in experimental animal models. Int J Pharm Res 2010;2:35–9.Search in Google Scholar
35. Daswani PD, Brijesh S, Tetali P, Antia NH, Birdi TJ. Antidiarrhoeal activity of Zingiber officinale (Rosc.) Curr Sci 2010;98:222–9.Search in Google Scholar
©2018 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Minireview
- Acorus calamus: a bio-reserve of medicinal values
- Behavior and Neuroprotection
- Electrodermal response to auditory stimuli in relation to menopausal transition period
- Reproduction
- First-line antituberculosis drugs disrupt endocrine balance and induce ovarian and uterine oxidative stress in rats
- Launaea taraxacifolia (Willd.) Amin ex C. Jeffrey inhibits oxidative damage and econucleotidase followed by increased cellular ATP in testicular cells of rats exposed to metropolitan polluted river water
- Cardiovascular Function
- Ameliorative effect of Azadirachta indica on sodium fluoride-induced hypertension through improvement of antioxidant defence system and upregulation of extracellular signal regulated kinase 1/2 signaling
- Regulation of hypoxia inducible factor/prolyl hydroxylase binding domain proteins 1 by PPARα and high salt diet
- Oxidative Stress
- Amla (Emblica officinalis) improves hepatic and renal oxidative stress and the inflammatory response in hypothyroid female wistar rats fed with a high-fat diet
- Metabolism
- Cocos nucifera water improves metabolic functions in offspring of high fat diet fed Wistar rats
- Prevalence of dry eye disease and its association with dyslipidemia
- Phytotherapy
- An isobolographic analysis of the anti-nociceptive effect of geraniin in combination with morphine or diclofenac
- Antidiarrheal and antimicrobial activities of the ethanol extract from the Icacina senegalensis root bark
- Chromatographic fingerprint analysis, antioxidant properties, and inhibition of cholinergic enzymes (acetylcholinesterase and butyrylcholinesterase) of phenolic extracts from Irvingia gabonensis (Aubry-Lecomte ex O’Rorke) Baill bark
- Corrigendum
- Corrigendum to: Possible modulation of PPAR-γ cascade against depression caused by neuropathic pain in rats
