Effects of peripherally and centrally applied ghrelin on the oxidative stress induced by renin angiotensin system in a rat model of renovascular hypertension
-
Vivian Boshra
Abstract
Background:
Renovascular hypertension (RVH) is a result of renal artery stenosis, which is commonly due to astherosclerosis. In this study, we aimed to clarify the central and peripheral effects of ghrelin on the renin-angiotensin system (RAS) in a rat model of RVH.
Methods:
RVH was induced in rats by partial subdiaphragmatic aortic constriction. Experiment A was designed to assess the central effect of ghrelin via the intracerebroventricular (ICV) injection of ghrelin (5 μg/kg) or losartan (0.01 mg/kg) in RVH rats. Experiment B was designed to assess the peripheral effect of ghrelin via the subcutaneous (SC) injection of ghrelin (150 μg/kg) or losartan (10 mg/kg) for 7 consecutive days. Mean arterial blood pressure (MAP), heart rate, plasma renin activity (PRA), and oxidative stress markers were measured in all rats. In addition, angiotensin II receptor type 1 (AT1R) concentration was measured in the hypothalamus of rats in Experiment B.
Results:
RVH significantly increased brain AT1R, PRA, as well as the brain and plasma oxidative stress. Either SC or ICV ghrelin or losartan caused a significant decrease in MAP with no change in the heart rate. Central ghrelin or losartan caused a significant decrease in brain AT1R with significant alleviation of the brain oxidative stress. Central ghrelin caused a significant decrease in PRA, whereas central losartan caused a significant increase in PRA. SC ghrelin significantly decreased PRA and plasma oxidative stress, whereas SC losartan significantly increased PRA and decreased plasma oxidative stress.
Conclusions:
The hypotensive effect of ghrelin is mediated through the amelioration of oxidative stress, which is induced by RAS centrally and peripherally.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2017 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Editorial
- The double face of light effects: circadian adjustment or disruption
- Review
- Artificial light-at-night – a novel lifestyle risk factor for metabolic disorder and cancer morbidity
- Behavior and Neuroprotection
- Anti-stress effects of a GSK-3β inhibitor, AR-A014418, in immobilization stress of variable duration in mice
- Cardiovascular Function
- Addition of omega-3 fatty acid and coenzyme Q10 to statin therapy in patients with combined dyslipidemia
- Oxidative Stress
- Protective effect of Moringa oleifera oil against HgCl2-induced hepato- and nephro-toxicity in rats
- Effects of peripherally and centrally applied ghrelin on the oxidative stress induced by renin angiotensin system in a rat model of renovascular hypertension
- Investigation of the role of α-lipoic acid on fatty acids profile, some minerals (zinc, copper, iron) and antioxidant activity against aluminum-induced oxidative stress in the liver of male rats
- Metabolism
- The choice of freely preferred cadence by trained nonprofessional cyclists may not be characterized by mechanical efficiency
- Immune Response
- Ascorbic acid does not modulate potassium currents in cultured human lymphocytes
- Phytotherapy
- Anti-inflammatory activity of Elaeagnus angustifolia fruit extract on rat paw edema
- Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats
- In vitro inhibition of phosphodiesterase-5 and arginase activities from rat penile tissue by two Nigerian herbs (Hunteria umbellata and Anogeissus leiocarpus)
- Antioxidant and antiproliferative potentials of methanol extract of Xylopia aethiopica (Dunal) A. Rich in PC-3 and LNCaP cells
Articles in the same Issue
- Frontmatter
- Editorial
- The double face of light effects: circadian adjustment or disruption
- Review
- Artificial light-at-night – a novel lifestyle risk factor for metabolic disorder and cancer morbidity
- Behavior and Neuroprotection
- Anti-stress effects of a GSK-3β inhibitor, AR-A014418, in immobilization stress of variable duration in mice
- Cardiovascular Function
- Addition of omega-3 fatty acid and coenzyme Q10 to statin therapy in patients with combined dyslipidemia
- Oxidative Stress
- Protective effect of Moringa oleifera oil against HgCl2-induced hepato- and nephro-toxicity in rats
- Effects of peripherally and centrally applied ghrelin on the oxidative stress induced by renin angiotensin system in a rat model of renovascular hypertension
- Investigation of the role of α-lipoic acid on fatty acids profile, some minerals (zinc, copper, iron) and antioxidant activity against aluminum-induced oxidative stress in the liver of male rats
- Metabolism
- The choice of freely preferred cadence by trained nonprofessional cyclists may not be characterized by mechanical efficiency
- Immune Response
- Ascorbic acid does not modulate potassium currents in cultured human lymphocytes
- Phytotherapy
- Anti-inflammatory activity of Elaeagnus angustifolia fruit extract on rat paw edema
- Histopathological and biochemical assessments of Costus afer stem on alloxan-induced diabetic rats
- In vitro inhibition of phosphodiesterase-5 and arginase activities from rat penile tissue by two Nigerian herbs (Hunteria umbellata and Anogeissus leiocarpus)
- Antioxidant and antiproliferative potentials of methanol extract of Xylopia aethiopica (Dunal) A. Rich in PC-3 and LNCaP cells
