Analgesic properties of aqueous leaf extract of Haematostaphis barteri: involvement of ATP-sensitive potassium channels, adrenergic, opioidergic, muscarinic, adenosinergic and serotoninergic pathways
-
Elvis Ofori Ameyaw
,
Ama Kyerea Thomford
Abstract
Background:
Pain is the most common cause of patients seeking medical advice as a result of its association with different pathologies. This study evaluated the antinociceptive property of Haematostaphis barteri as well as the possible mechanism(s) associated with its antinociceptive property.
Methods:
Mice were administered H. barteri (30–300 mg kg−1; p.o.), followed by intraplantar injection of 10 μL of 5% formalin into the hind paws. The pain score was determined for 1 h in the formalin test. The possible nociceptive pathways involved in the antinociceptive action of H. barteri were determined by pre-treating mice with theophylline (5 mg kg−1, a non-selective adenosine receptor antagonist), naloxone (2 mg kg−1, a non-selective opioid receptor antagonist), glibenclamide (8 mg kg−1; an ATP-sensitive K+ channel inhibitor), and atropine (3 mg kg−1; non-selective muscarinic antagonist).
Results:
H. barteri (30–300 mg kg−1) significantly and dose dependently precluded both first and second phases of nociception. Pre-treatment with naloxone had no effect on the analgesic activities of H. barteri in the first phase. Again, pre-treatment with atropine and glibenclamide did not significantly reverse the neurogenic antinociception of the extract in phase 1. However, theophylline reversed the analgesic effect of the extract in the first phase. In phase 2, theophylline had no effect on the analgesic activities of the extract. Naloxone, atropine, and glibenclamide significantly blocked the antinociception of H. barteri in the inflammatory phase of the formalin test.
Conclusions:
H. barteri possesses antinociceptive property mediated via the opioidergic, adrenergic, muscarinic, ATP-sensitive K+ channels, and adenosinergic nociceptive pathways.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
References
1. Jagerovic N, Cano C, Elguero J, Goya P, Callado LF, Meana JJ, et al. Long-acting fentanyl analogues: synthesis and pharmacology of N-(1-phenylpyrazolyl)-N-(1-phenylalkyl-4-piperidyl) propanamides. Bioorg Med Chem 2002;10:817–27.10.1016/S0968-0896(01)00345-5Search in Google Scholar
2. National Academies Press. Relieving Pain in America: A Blueprint for Transforming Prevention, Care, Education, and Research. Institute of Medicine (US) Committee on Advancing Pain Research, Care, and Education. Washington, DC, 2011.Search in Google Scholar
3. Rang HP, Dale MN. Analgesic drugs. In: Rang HP, Dale MN, Ritter JM, Flower RJ, editors. Rang and Dale’s pharmacology, 6th ed. Edinburgh, London, New York, Oxford, Philadelphia, St. Louis, Sydney, Tronto: Elsevier Churchill Livingstone, 2012:588–96.10.1016/B978-0-443-06911-6.50046-3Search in Google Scholar
4. Bruehl S, Apkarian AV, Ballantyne JC, Berger A, Borsook D, Chen WG, et al. Personalized medicine and opioid analgesic prescribing for chronic pain: opportunities and challenges. J Pain 2013;14:103–13.10.1016/j.jpain.2012.10.016Search in Google Scholar
5. Candiotti KA, Gitlin MC. Review of the effect of opioid-related side effects on the undertreatment of moderate to severe chronic non-cancer pain: tapentadol, a step toward a solution? Curr Med Res Opin 2010;26:1677–84.10.1185/03007995.2010.483941Search in Google Scholar
6. Chandok N, Watt KD. Pain management in the cirrhotic patient: the clinical challenge. Mayo Clin Proc 2010;85:451–8.10.4065/mcp.2009.0534Search in Google Scholar
7. Srivastav S, Singh P, Mishra G, Jha KK, Khosa RL. Achyranthes aspera – an important medicinal plant: a review. J Nat Prod Plant Resources 2011;1:1–14.Search in Google Scholar
8. Tijani Y, Uguru MO, Salawu OA. Anti-pyretic, anti-inflammatory and anti-diarrhoeal properties of Faidherbia albida in rats. African J Biotechnol 2008;7:696–700.Search in Google Scholar
9. Asase A, Kokubun T, Grayer RJ, Kite G, Simmonds MS, Oteng-Yeboah AA. Chemical constituents and antimicrobial activity of medicinal plants from Ghana: Cassia sieberiana, Haematostaphis barteri, and Pseudocedrela kotschyi. Phytother Res 2008;22:1013–1016.10.1002/ptr.2392Search in Google Scholar
10. Woode E, Ameyaw EO, Ainooson GK, Abotsi WM, Gyasi EB, Kyekyeku JO. Analgesic effects of an ethanol extract of the fruits of xylopia aethiopica and xylopic acid in murine models of pain: possible mechanism(s). Pharmacologia 2013;4:1–13.10.5567/pharmacologia.2013.285.300Search in Google Scholar
11. Meunier CJ, Burton J, Cumps J, Verbeeck KR. Evaluation of the formalin test to assess the analgesic activity of diflunisal in the rat. Eur J Pharm Sci 1998;6:307–12.10.1016/S0928-0987(97)10020-3Search in Google Scholar
12. Coderre TJ, Abbott FV, Sawynok J. Formalin test. In: Gebhart GF, Schmidt, Robert F, editors. Encyclopaedia of Pain. 2nd Edition. Springer Berlin Heidelberg 2013:1303–8.10.1007/978-3-642-28753-4_1535Search in Google Scholar
13. Shields SD, Cavanaugh DJ, Lee H, Anderson DJ, Basbaum AI. Pain behavior in the formalin test persists after ablation of the great majority of C-fiber nociceptors. Pain 2010;151:422–9.10.1016/j.pain.2010.08.001Search in Google Scholar PubMed PubMed Central
14. Vanderah TW. Delta and kappa opioid receptors as suitable drug targets for pain. Clin J Pain 2010;26 (Suppl 10):S10–5.10.1097/AJP.0b013e3181c49e3aSearch in Google Scholar PubMed
15. Al-Hasani R, Bruchas MR. Molecular mechanisms of opioid receptor-dependent signaling and behavior. Anesthesiology 2011;115:1363–81.10.1097/ALN.0b013e318238bba6Search in Google Scholar
16. Sawynok J. Adenosine receptor activation and nociception. Eur J Pharmacol 1998;347:1–11.10.1016/S0014-2999(97)01605-1Search in Google Scholar
17. Berg KA, Patwardhan AM, Akopian AN. Receptor and channel heteromers as pain targets. Pharmaceuticals (Basel) 2012;5:249–78.10.3390/ph5030249Search in Google Scholar PubMed PubMed Central
18. Bouaziz H, Hewitt C, Eisenach JC. Subarachnoid neostigmine potentiation of alpha 2-adrenergic agonist analgesia. Dexmedetomidine versus clonidine. Reg Anesth 1995;20:121–7.Search in Google Scholar
©2016 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Analgesic properties of aqueous leaf extract of Haematostaphis barteri: involvement of ATP-sensitive potassium channels, adrenergic, opioidergic, muscarinic, adenosinergic and serotoninergic pathways
- Correlative study of peripheral ATP1A1 gene expression level to anxiety severity score on major depressive disorder patients
- Comparison of fluoxetine and 1-methyl-L-tryptophan in treatment of depression-like illness in Bacillus Calmette-Guerin-induced inflammatory model of depression in mice
- Reproduction
- Tobacco smoke exposure induces irreversible alteration of testicular function in prepubertal rats
- Oxidative Stress
- α-Lipoic acid attenuates transplacental nicotine-induced germ cell and oxidative DNA damage in adult mice
- Effects of nicotine in the presence and absence of vitamin E on morphology, viability and osteogenic gene expression in MG-63 osteoblast-like cells
- Metabolism
- Age-dependent features of CYP3A, CYP2C, and CYP2E1 functioning at metabolic syndrome
- Exercise increases the level of plasma orexin A in humans
- Inflammation
- Drug combinations in diabetic neuropathic pain: an experimental validation
- Pharmacokinetics of ceftriaxone in patients undergoing continuous renal replacement therapy
- Hematology
- Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states
- Protection against arsenic-induced hematological and hepatic anomalies by supplementation of vitamin C and vitamin E in adult male rats
- One more health benefit of blood donation: reduces acute-phase reactants, oxidants and increases antioxidant capacity
- Phytotherapy
- Antithrombotic and cytotoxic activities of four Bangladeshi plants and PASS prediction of their isolated compounds
