Protection against arsenic-induced hematological and hepatic anomalies by supplementation of vitamin C and vitamin E in adult male rats
-
Rubia Mondal
,
Aparna Mukhopadhyay
Abstract
Background:
Chronic arsenic exposure via contaminated drinking water is a global environmental health problem associated with hematological, hepatic and many serious systemic disorders. This study on adult male rats evaluated the protective effects of vitamin E (VE) and vitamin C (VC) against arsenic-mediated hematological and hepatic toxicities.
Methods:
Arsenic was administered orally as arsenic trioxide (3 mg/kg body weight/day), as a single dose for 30 consecutive days or along with VC/ascorbic acid (200 mg/kg body weight/day dissolved in water) and VE/α-tocopherol (400 mg/kg body weight/day dissolved in olive oil) as supplements. Multiple hematological and hepatic parameters were assessed.
Results:
Arsenic exposure caused significant reduction of erythrocyte counts (p<0.05), leukocyte counts (p<0.01) and hemoglobin (Hb) levels (p<0.01). Arsenic exposure also led to marked echinocytic transformation of erythrocytes resulting in increased morphological index (p<0.001). Altered serum oxidative balance was observed with a higher oxidative stress index (p<0.001). The results also showed a significant increase of serum cholesterol (p<0.05), low-density lipoprotein (p<0.001) and triglycerides (p<0.01), and decreased high-density lipoprotein (p<0.01) along with total protein (p<0.01). A marked elevation of hepatic thiobarbituric acid reactive substance (p<0.05) along with decreased reduced glutathione (p<0.001) levels were also observed. Interestingly, co-administration of VC and VE significantly prevented all the arsenic-induced alterations (p<0.05) except Hb content and serum protein.
Conclusions:
The present investigation offers strong evidence regarding the protective efficacy of co-administration of VC and VE against hematotoxicity and hepatotoxicity in adult male rats caused by chronic arsenic exposure.
Acknowledgments
The authors gratefully acknowledge the financial support provided by the Faculty Research and Professional Development Fund from Presidency University.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding:None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2016 Walter de Gruyter GmbH, Berlin/Boston
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- Comparison of fluoxetine and 1-methyl-L-tryptophan in treatment of depression-like illness in Bacillus Calmette-Guerin-induced inflammatory model of depression in mice
- Reproduction
- Tobacco smoke exposure induces irreversible alteration of testicular function in prepubertal rats
- Oxidative Stress
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- Pharmacokinetics of ceftriaxone in patients undergoing continuous renal replacement therapy
- Hematology
- Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states
- Protection against arsenic-induced hematological and hepatic anomalies by supplementation of vitamin C and vitamin E in adult male rats
- One more health benefit of blood donation: reduces acute-phase reactants, oxidants and increases antioxidant capacity
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Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Analgesic properties of aqueous leaf extract of Haematostaphis barteri: involvement of ATP-sensitive potassium channels, adrenergic, opioidergic, muscarinic, adenosinergic and serotoninergic pathways
- Correlative study of peripheral ATP1A1 gene expression level to anxiety severity score on major depressive disorder patients
- Comparison of fluoxetine and 1-methyl-L-tryptophan in treatment of depression-like illness in Bacillus Calmette-Guerin-induced inflammatory model of depression in mice
- Reproduction
- Tobacco smoke exposure induces irreversible alteration of testicular function in prepubertal rats
- Oxidative Stress
- α-Lipoic acid attenuates transplacental nicotine-induced germ cell and oxidative DNA damage in adult mice
- Effects of nicotine in the presence and absence of vitamin E on morphology, viability and osteogenic gene expression in MG-63 osteoblast-like cells
- Metabolism
- Age-dependent features of CYP3A, CYP2C, and CYP2E1 functioning at metabolic syndrome
- Exercise increases the level of plasma orexin A in humans
- Inflammation
- Drug combinations in diabetic neuropathic pain: an experimental validation
- Pharmacokinetics of ceftriaxone in patients undergoing continuous renal replacement therapy
- Hematology
- Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states
- Protection against arsenic-induced hematological and hepatic anomalies by supplementation of vitamin C and vitamin E in adult male rats
- One more health benefit of blood donation: reduces acute-phase reactants, oxidants and increases antioxidant capacity
- Phytotherapy
- Antithrombotic and cytotoxic activities of four Bangladeshi plants and PASS prediction of their isolated compounds
