Effects of nicotine in the presence and absence of vitamin E on morphology, viability and osteogenic gene expression in MG-63 osteoblast-like cells
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Maryam Torshabi
Abstract
Background:
Evidence shows that oxidative stress induced by nicotine plays an important role in bone loss. Vitamin E with its antioxidative properties may be able to reverse the effects of nicotine on bone. This study aimed to assess the effects of nicotine in the presence and absence of vitamin E on morphology, viability and osteogenic gene expression in MG-63 (osteosarcoma) human osteoblast-like cells.
Methods:
We treated the cells with 5 mM nicotine. The viability and morphology of cells were evaluated respectively using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) and crystal violet assays. The effect of nicotine on osteogenic gene expression in MG-63 cells was assessed by real-time reverse-transcription polymerase chain reaction of osteoblast markers, namely, alkaline phosphatase, osteocalcin and bone sialoprotein.
Results:
The results revealed that survival and proliferation of MG-63 cells were suppressed following exposure to nicotine, and cytoplasm vacuolization occurred in the cells. Nicotine significantly down-regulated the expression of osteogenic marker genes. Such adverse effects on morphology, viability and osteogenic gene expression of MG-63 cells were reversed by vitamin E therapy.
Conclusions:
In conclusion, vitamin E supplementation may play a role in proliferation and differentiation of osteoblasts, and vitamin E can be considered as an anabolic agent to treat nicotine-induced bone loss.
Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
Competing interests: The funding organization(s) played no role in the study design; in the collection, analysis, and interpretation of data; in the writing of the report; or in the decision to submit the report for publication.
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©2016 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Analgesic properties of aqueous leaf extract of Haematostaphis barteri: involvement of ATP-sensitive potassium channels, adrenergic, opioidergic, muscarinic, adenosinergic and serotoninergic pathways
- Correlative study of peripheral ATP1A1 gene expression level to anxiety severity score on major depressive disorder patients
- Comparison of fluoxetine and 1-methyl-L-tryptophan in treatment of depression-like illness in Bacillus Calmette-Guerin-induced inflammatory model of depression in mice
- Reproduction
- Tobacco smoke exposure induces irreversible alteration of testicular function in prepubertal rats
- Oxidative Stress
- α-Lipoic acid attenuates transplacental nicotine-induced germ cell and oxidative DNA damage in adult mice
- Effects of nicotine in the presence and absence of vitamin E on morphology, viability and osteogenic gene expression in MG-63 osteoblast-like cells
- Metabolism
- Age-dependent features of CYP3A, CYP2C, and CYP2E1 functioning at metabolic syndrome
- Exercise increases the level of plasma orexin A in humans
- Inflammation
- Drug combinations in diabetic neuropathic pain: an experimental validation
- Pharmacokinetics of ceftriaxone in patients undergoing continuous renal replacement therapy
- Hematology
- Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states
- Protection against arsenic-induced hematological and hepatic anomalies by supplementation of vitamin C and vitamin E in adult male rats
- One more health benefit of blood donation: reduces acute-phase reactants, oxidants and increases antioxidant capacity
- Phytotherapy
- Antithrombotic and cytotoxic activities of four Bangladeshi plants and PASS prediction of their isolated compounds
Articles in the same Issue
- Frontmatter
- Behavior and Neuroprotection
- Analgesic properties of aqueous leaf extract of Haematostaphis barteri: involvement of ATP-sensitive potassium channels, adrenergic, opioidergic, muscarinic, adenosinergic and serotoninergic pathways
- Correlative study of peripheral ATP1A1 gene expression level to anxiety severity score on major depressive disorder patients
- Comparison of fluoxetine and 1-methyl-L-tryptophan in treatment of depression-like illness in Bacillus Calmette-Guerin-induced inflammatory model of depression in mice
- Reproduction
- Tobacco smoke exposure induces irreversible alteration of testicular function in prepubertal rats
- Oxidative Stress
- α-Lipoic acid attenuates transplacental nicotine-induced germ cell and oxidative DNA damage in adult mice
- Effects of nicotine in the presence and absence of vitamin E on morphology, viability and osteogenic gene expression in MG-63 osteoblast-like cells
- Metabolism
- Age-dependent features of CYP3A, CYP2C, and CYP2E1 functioning at metabolic syndrome
- Exercise increases the level of plasma orexin A in humans
- Inflammation
- Drug combinations in diabetic neuropathic pain: an experimental validation
- Pharmacokinetics of ceftriaxone in patients undergoing continuous renal replacement therapy
- Hematology
- Phenotypic homogeneity with minor deviance in osmotic fragility of Sahel goat erythrocytes in non-ionic sucrose media during various physiologic states
- Protection against arsenic-induced hematological and hepatic anomalies by supplementation of vitamin C and vitamin E in adult male rats
- One more health benefit of blood donation: reduces acute-phase reactants, oxidants and increases antioxidant capacity
- Phytotherapy
- Antithrombotic and cytotoxic activities of four Bangladeshi plants and PASS prediction of their isolated compounds
