Physiological characteristics of crab Portunus sanguinolentus egg mass extract from southeast coast of India
-
Subramanian Bragadeeswaran
,
Nadarajah Sri Kumaran
Abstract
Background: The present investigation was undertaken to evaluate the physiological characteristics of crab egg mass (Portunus sanguinolentus) crude extract.
Methods: Assays were followed by standard methods.
Results: A maximum of 256 hemolytic units (HU) and a minimum of 128 HU were found in chicken and goat erythrocytes. In an antimicrobial assay, Salmonella paratyphi and Aspergillus niger showed most susceptibility (8.0 mm and 13.0 mm) against n-butanol extracts. In the tail flick method, 5.33±0.19* AR the maximum analgesic response was recorded 30 min after the administration of crude extract (150 mg kg–1). In the hot plate method, 5.13±0.13* AR maximum paw licking was recorded 15 min after the administration of crude extract (150 mg kg–1). In an anti-inflammatory assay, 0.31±0.23* anti-inflammatory response and inhibition of paw edema 39.22% were observed 1 h after the administration of crude extract (250 mg kg–1). Primary structural groups gained through Fourier transformed infrared (FTIR) analysis peaks were observed and assigned as amide groups I (1517.81–1633.12 cm–1), II (1446.58–1517.81 cm–1) and III (1342.47–1406.85 cm–1).
Conclusions: The results indicate that crab egg masses have remarkable antimicrobial, hemolytic and cytotoxic activities.
References
1. Iwanaga S, Lee BL. Recent advances in the innate immunity of invertebrate animals. J Biochem Mol Biol 2005;38:128–50.10.5483/BMBRep.2005.38.2.128Search in Google Scholar
2. Hancock RE, Brown KL, Mookherjee N. Host defence peptides from invertebrates emerging antimicrobial strategies. Immunobiology 2006;211:315–22.10.1016/j.imbio.2005.10.017Search in Google Scholar
3. Arrieta MC, Leskiw BK, Kaufman WR. Antimicrobial activity in the egg wax of the African cattle tick Amblyomma hebraeum (Acari: Ixodidae). Exp Appl Acarol 2006;39:297–13.10.1007/s10493-006-9014-5Search in Google Scholar
4. Fernando SA, Fernando OJ. A field guide to the common invertebrates of the east coast of India. Centre of Advanced Study in Marine Biology, Annamalai University, 2002:63.Search in Google Scholar
5. Bradford MM. A rapid and sensitive method for the quantification of microgram quantities of protein using the principle of protein dye binding. Anal Biochem 1976;72:248–54.10.1016/0003-2697(76)90527-3Search in Google Scholar
6. Bragadeeswaran S, Ganesan K, Sri Kumaran N. Hemolytic activities from ascidian Polyclinum madrasensis Sebestian, 1952 and Phallusia nigra Savigny, 1816 from Tuticorin coast of India. Asian J Appl Sci 2011;4:630–9.10.3923/ajaps.2011.630.639Search in Google Scholar
7. Meyer BN, Ferrigi NR, Putnam JE, Jacobson LB, Nicolas DE, Mclaughin JL. Brine shrimp: a convenient general bioassay for active plant constituents. Planta Med 1982;45:31–4.10.1055/s-2007-971236Search in Google Scholar PubMed
8. Laouer H, Meriem EK, Parado S, Baldovini N. An antibacterial and antifungal phenylpropanoid from Carum montaum (Coss. et Dur.) Benth. et Hook. Phytother Res 2009;23:1726–30.10.1002/ptr.2820Search in Google Scholar PubMed
9. National Committee for Clinical Laboratory Standards. Method for antifungal disk diffusion susceptibility testing in yeasts. Approved guideline M-44-A. Wayne, PA: CLSI/National Committee for Clinical Laboratory Standards, 2006;M100-S16.Search in Google Scholar
10. Al-Ali A, Alkhawajah A, Randhawa MA, Shaikh NA. Oral and intraperitoneal LD50 of thymoquinone, an active principle of Nigella sativa, in mice and rats. J Ayub Med Coll Abbotabad 2008;20:25–7.Search in Google Scholar
11. Miller LC, Tainter ML. Estimation of LD50 and its error by means of log-probit graph paper. Proc Soc Exp Bio Med 1944;57:261–4.10.3181/00379727-57-14776Search in Google Scholar
12. Sutha D, Azadeh SE, Sabariah I, Surash R, Mun FY. Evaluation of the antinociceptive activity and acute oral toxicity of standardized ethanolic extract of the rhizome of Curcuma xanthorrhiza Roxb. Molecules 2010;15:2925–34.10.3390/molecules15042925Search in Google Scholar PubMed PubMed Central
13. Jung HJ, Song YS, Lim CJ, Park EH. Anti-inflammatory, anti-angiogenic and anti-nociceptive activities of an ethanol extract of Salvia plebeia R. Brown. J Ethnopharmacol 2009;126:355–60.10.1016/j.jep.2009.08.031Search in Google Scholar PubMed
14. Thangaraj S, Bragadeeswaran S. Assessment of biomedical and pharmacological activities of sea anemones Stichodactyla mertensii and Stichodactyla gigantea from Gulf of Mannar Biosphere Reserve, southeast coast of India. J Venom Anim Toxins incl Trop Dis 2012;18:53–61.10.1590/S1678-91992012000100007Search in Google Scholar
15. Sri Kumaran N, Bragadeeswaran S, Balasubramanian T, Meenakshi VK. Bioactivity potential of extracts from ascidian Lissoclinum fragile. Afr J Pharmacy Pharmacol 2012;6:1854–9.10.5897/AJPP12.277Search in Google Scholar
16. Hussain SM, Ananthan G, Sivaperumal P. Exploration of cytotoxic potential from marine ascidians Trididemnum clinides and Trididemnum savignii. J Pharmacy Res 2011;4:2032–3.Search in Google Scholar
17. Prasath Sankar P. Bioactive potential of sea urchin Temnopleurus toreumaticus from Devanampattinam, southeast coast of India. MSc thesis, Annamalai University, 2011.Search in Google Scholar
18. Stewart JE, Zwicker BM. Natural and induced bactericidal activities in the hemolymph of the lobster; Homarus americanus: product of hemocyte-plasma interactions. Can J Microbiol 1972;18:1499–509.10.1139/m72-229Search in Google Scholar PubMed
19. Benkendorff K, Bremner JB, Davis AR. Tyrian purple precursors in the egg masses of the Australian muricid Dicathais orbita: a possible defensive role. J Chem Ecol 2000;26:1037–50.10.1023/A:1005441116095Search in Google Scholar
20. Veeruraj A, Ravichandran S, Rameshkumar G. Antibacterial activity of crab haemolymph on clinical pathogens. Trends Appl Sci Res 2008;3:174–81.10.3923/tasr.2008.174.181Search in Google Scholar
21. Gopalakrishnan S, Meenakshi VK, Shanmuga Priya D. Antipyretic and analgesic activity of Phallusia nigra, Savigny, 1816. Ann Biol Res 2011;2:192–6.Search in Google Scholar
22. Neves SA, Freitas AL, Sousa BW, Rocha ML, Correia MV, Sampaio DA, et al. Antinociceptive properties in mice of lectin isolated from the marine alga Amansia multifida Lamouroux. J Braz Med Biol Res 2007;40:127–34.10.1590/S0100-879X2007000100016Search in Google Scholar
23. Suganthi K, Bragadeeswaran S, Sri Kumaran N, Thangaraj S, Balasubramanian T. Biological and pharmacological activities of jelly fish Crambionella stuhalmanni (Chun, 1896) and Chrysaora quinquecirrha (Desor, 1848). J Pharm Pharm Sci 2011;3:230–6.Search in Google Scholar
24. Gautam R, Jachak SM. Recent developments in anti-inflammatory natural products. Med Res Rev 2009;29:767–20.10.1002/med.20156Search in Google Scholar PubMed
25. Dang HT, Lee HJ, Yoo ES, Shinde PB, Lee YM, Hong J, et al. Anti-inflammatory constituents of the red alga Gracilaria verrucosa and their synthetic analogues. J Nat Prod 2008;71: 232–40.10.1021/np070452qSearch in Google Scholar PubMed
26. Park YK, Rasmussen HE, Ehler SJ, Blobaum KR, Lu F, Schlegel VL, et al. Repression of proinflammatory gene expression by lipid extract of Nostoc commune var sphaeroides Kutzing, a blue-green alga, via inhibition of nuclear factor-kappaB in RAW 264.7 macrophages. Nutr Res 2008;28:83–91.10.1016/j.nutres.2007.11.008Search in Google Scholar PubMed
27. Hussein G, Sankawa U, Gato H, Matsumoto K, Watanabe H. Astaxanthin, a carotenoid with potential in human health and nutrition. J Nat Prod 2006;69:443–9.10.1021/np050354+Search in Google Scholar PubMed
©2015 by De Gruyter
Articles in the same Issue
- Frontmatter
- Genotoxicity and Cytotoxicity
- Randomized, double-blind, placebo-controlled study to investigate the pharmacodynamic interaction of 5-HT3 antagonist ondansetron and paracetamol in postoperative patients operated in an ENT department under local anesthesia
- Influence of vitamin E and quercetin on Nigerian Bonny Light crude oil-induced neuronal and testicular toxicity in Wistar rats
- Vascular Conditions
- Reduction of pressor response to stress by centrally acting apelin in spontaneously hypertensive rats
- Oxidative Stress
- Protective effects of blackberry and quercetin on sodium fluoride-induced oxidative stress and histological changes in the hepatic, renal, testis and brain tissue of male rat
- Modulatory effects of cod liver oil on the antioxidant status and oxidative stress induced by acute exposure to carbon tetrachloride (CCL4) in experimental animal models
- Hematological Profile
- Acute high blood glucose level attenuates histamine-stimulated acid secretion in male Wistar rats
- Inflammation
- Physiological characteristics of crab Portunus sanguinolentus egg mass extract from southeast coast of India
- Phytotherapy
- Attenuating effect of standardized lyophilized Cinnamomum zeylanicum bark extract against streptozotocin-induced experimental dementia of Alzheimer’s type
- Erythrina lysistemon-derived flavonoids account only in part for the plant’s specific effects on rat uterus and vagina
- A prospective drug use evaluation of antihypertensive drugs in in-patients of a tertiary referral care hospital
- Role for monoaminergic systems in the antidepressant and anxiolytic properties of the hydroethanolic leaf extract from Adenia cissampeloides
- Short Communication
- Anti-inflammatory effects of aqueous extract of Mangifera indica in Wistar rats
Articles in the same Issue
- Frontmatter
- Genotoxicity and Cytotoxicity
- Randomized, double-blind, placebo-controlled study to investigate the pharmacodynamic interaction of 5-HT3 antagonist ondansetron and paracetamol in postoperative patients operated in an ENT department under local anesthesia
- Influence of vitamin E and quercetin on Nigerian Bonny Light crude oil-induced neuronal and testicular toxicity in Wistar rats
- Vascular Conditions
- Reduction of pressor response to stress by centrally acting apelin in spontaneously hypertensive rats
- Oxidative Stress
- Protective effects of blackberry and quercetin on sodium fluoride-induced oxidative stress and histological changes in the hepatic, renal, testis and brain tissue of male rat
- Modulatory effects of cod liver oil on the antioxidant status and oxidative stress induced by acute exposure to carbon tetrachloride (CCL4) in experimental animal models
- Hematological Profile
- Acute high blood glucose level attenuates histamine-stimulated acid secretion in male Wistar rats
- Inflammation
- Physiological characteristics of crab Portunus sanguinolentus egg mass extract from southeast coast of India
- Phytotherapy
- Attenuating effect of standardized lyophilized Cinnamomum zeylanicum bark extract against streptozotocin-induced experimental dementia of Alzheimer’s type
- Erythrina lysistemon-derived flavonoids account only in part for the plant’s specific effects on rat uterus and vagina
- A prospective drug use evaluation of antihypertensive drugs in in-patients of a tertiary referral care hospital
- Role for monoaminergic systems in the antidepressant and anxiolytic properties of the hydroethanolic leaf extract from Adenia cissampeloides
- Short Communication
- Anti-inflammatory effects of aqueous extract of Mangifera indica in Wistar rats
