Temporal expression of IL-1β and IL-10 in rat skin, muscle, small bowel, and colon wounds: a correlative study
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Mohammed A. Alzoghaibi
,
Abdullah I. Al-Oraini
Abstract
Background: Cytokines play a major role in coordinated wound healing events. We hypothesized that rapid intestinal healing is due to an early upregulation of the pro-inflammatory cytokine interleukin-1β (IL-1β), followed by increases in the expression of the anti-inflammatory cytokine IL-10.
Methods: We characterized the time course of IL-1β and IL-10 release at four wounds (skin, muscle, small bowel, and colonic anastomosis) after surgery on 38 juvenile male Sprague-Dawley rats. The tissue samples of each site were harvested at 0 (control), 1, 3, 5, 7, and 14 days postoperatively (n=6–8 per group) and analyzed by enzyme-linked immunosorbent assay kits for IL-1β and IL-10.
Results: IL-1β expression peaked at days 5 and 7 in small bowel and colonic wounds when compared to skin or muscle. Similarly, IL-10 showed high expression in these time points in small bowel and colonic wounds. However, IL-10 showed the same expression in all time points in muscle and skin tissues except at day 1.
Conclusions: The high expression in IL-1β and IL-10 levels in small bowel and colon might explain the accelerated healing process in these wounds in comparison to skin and muscle tissues. Additional studies are required to determine whether IL-1β and IL-10 expression is the major factor defining site-specific differences in healing rates in different tissues. Understanding cytokine action in the wound healing process could lead to novel and effective therapeutic strategies.
Acknowledgments
This work was supported by King Abdulaziz City for Science and Technology (KACST) Grant number: “gs1”ARP-28-82. We would like to thank Mr. Sabirin S. for his help in running the samples.
Conflict of interest statement
Authors’ conflict of interest disclosure: The authors stated that there are no conflicts of interest regarding the publication of this article.
Research funding: None declared.
Employment or leadership: None declared.
Honorarium: None declared.
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©2014 by Walter de Gruyter Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Review
- Cerebral stroke injury: the role of cytokines and brain inflammation
- Cardiovascular-Pulmonary Interactions
- Gene functional dynamics: environment as a trigger?
- Arrhythmogenesis after acute myocardial necrosis with and without preceding ischemia in rats
- Measuring cardiopulmonary parameters during dual-task while walking
- Calcium-dependent mechanisms mediate the vasorelaxant effects of Tridax procumbens (Lin) aqueous leaf extract in rat aortic ring
- Inflammation
- Effects of cyclosporine and phenytoin on biomarker expressions in gingival fibroblasts of children and adults: an in vitro study
- Anti-inflammatory and analgesic activities of the methanol leaf extract of Phyllanthus amarus in some laboratory animals
- Oxidative Stress
- Ameliorative effect of diallyl trisulphide on arsenic-induced oxidative stress in rat erythrocytes and DNA damage in lymphocytes
- Anticholinesterase and antioxidative properties of water-extractable phytochemicals from some citrus peels
- Immune Response
- Temporal expression of IL-1β and IL-10 in rat skin, muscle, small bowel, and colon wounds: a correlative study
- Augmented primary humoral immune response and decreased cell-mediated immunity by Murraya koenigii in rats
- Analgesic, anti-inflammatory, and heme biomineralization inhibitory properties of Entada africana ethanol leaf extract with antiplasmodial activity against Plasmodium falciparum
- Hematological Profile
- Hematological profile in neonatal jaundice
- Effects of blood lead level on biochemical and hematological parameters in children with neurological diseases of Western Maharashtra, India
- Phytotherapy
- Antipsychotic property of solvent-partitioned fractions of Lonchocarpus cyanescens leaf extract in mice
- Aphrodisiac effect of aqueous root extract of Lecaniodiscus cupanioides in sexually impaired rats
- Virgin coconut oil protects against liver damage in albino rats challenged with the anti-folate combination, trimethoprim-sulfamethoxazole
- Metabolism
- Evaluation of in vitro aldose reductase inhibitory potential of alkaloidal fractions of Piper nigrum, Murraya koenigii, Argemone mexicana, and Nelumbo nucifera
Artikel in diesem Heft
- Frontmatter
- Review
- Cerebral stroke injury: the role of cytokines and brain inflammation
- Cardiovascular-Pulmonary Interactions
- Gene functional dynamics: environment as a trigger?
- Arrhythmogenesis after acute myocardial necrosis with and without preceding ischemia in rats
- Measuring cardiopulmonary parameters during dual-task while walking
- Calcium-dependent mechanisms mediate the vasorelaxant effects of Tridax procumbens (Lin) aqueous leaf extract in rat aortic ring
- Inflammation
- Effects of cyclosporine and phenytoin on biomarker expressions in gingival fibroblasts of children and adults: an in vitro study
- Anti-inflammatory and analgesic activities of the methanol leaf extract of Phyllanthus amarus in some laboratory animals
- Oxidative Stress
- Ameliorative effect of diallyl trisulphide on arsenic-induced oxidative stress in rat erythrocytes and DNA damage in lymphocytes
- Anticholinesterase and antioxidative properties of water-extractable phytochemicals from some citrus peels
- Immune Response
- Temporal expression of IL-1β and IL-10 in rat skin, muscle, small bowel, and colon wounds: a correlative study
- Augmented primary humoral immune response and decreased cell-mediated immunity by Murraya koenigii in rats
- Analgesic, anti-inflammatory, and heme biomineralization inhibitory properties of Entada africana ethanol leaf extract with antiplasmodial activity against Plasmodium falciparum
- Hematological Profile
- Hematological profile in neonatal jaundice
- Effects of blood lead level on biochemical and hematological parameters in children with neurological diseases of Western Maharashtra, India
- Phytotherapy
- Antipsychotic property of solvent-partitioned fractions of Lonchocarpus cyanescens leaf extract in mice
- Aphrodisiac effect of aqueous root extract of Lecaniodiscus cupanioides in sexually impaired rats
- Virgin coconut oil protects against liver damage in albino rats challenged with the anti-folate combination, trimethoprim-sulfamethoxazole
- Metabolism
- Evaluation of in vitro aldose reductase inhibitory potential of alkaloidal fractions of Piper nigrum, Murraya koenigii, Argemone mexicana, and Nelumbo nucifera
