Production and Preliminary Characterization of Antioxidant Polysaccharide by Submerged Culture of Culinary and Medicinal Fungi Cordyceps militaris CICC14013
-
Wan Chen
Abstract
Polysaccharides from Cordyceps militaris show multiple biological activities, such as antioxidation, antitumor, antivirus and immunomodulating properties, etc. The nutritional requirements for antioxidant polysaccharide bio-synthesis by submerged culture of C. militaris were evaluated on the basis of assay for DPPH radical scavenging ability and reducing power in this work. The results showed the best antioxidant activity of polysaccharide could be achieved when the media consisted of mannose, peptone, MgSO4, Na2HPO4, and KH2PO4. Furthermore, the main fraction of antioxidant polysaccharide, CMP-2, was preliminary isolated from the fermentation broth of C. militaris by ethanol precipitation, filtration, and DEAE cellulose-52 chromatography. CMP-2 was composed of mannose, rhamnose, glucose and galactose in a ratio of 2527:164:172:100, and had an average molecular weight (Mw) of approximately 8175 Da. The results suggested antioxidant polysaccharide could be produced by submerged culture of C. militaris and the medium composition significantly affected its DPPH radical scavenging ability and reducing power.
Funding statement: This work was supported by Zhejiang Department of Science and Technology (Project No. 2015C32131) and Program for New Century Excellent Talents in University (Project No. NCET-13-1046).
References
1. Tabaraki R, Nateghi A. Optimization of ultrasonic-assisted extraction of natural antioxidants from rice bran using response surface methodology. Ultrason Sonochem 2011;18:1279–86.10.1016/j.ultsonch.2011.05.004Search in Google Scholar
2. Tan LH, Zhang D, Yu B, Zhao SP, Wang JW, Yao L, et al. Antioxidant activity and optimization of extraction of polysaccharide from the roots of Dipsacus asperoides. Int J Biol Macromol 2015;81:332–9.10.1016/j.ijbiomac.2015.08.022Search in Google Scholar
3. Zhang CH, Yu Y, Liang YZ, Chen XQ. Purification, partial characterization and antioxidant activity of polysaccharides from Glycyrrhiza uralensis. Int J Biol Macromol 2015;79:681–6.10.1016/j.ijbiomac.2015.05.060Search in Google Scholar
4. Iverson F. Phenolic antioxidants: health protection branch studies on butylated hydroxyanisole. Cancer Lett 1995;93:49–54.10.1016/0304-3835(95)03787-WSearch in Google Scholar
5. Xiao JH, Xiao DM, Chen DX, Xiao Y, Liang ZQ, Zhong JJ. Polysaccharides from the medicinal mushroom Cordyceps taii show antioxidant and immunoenhancing activities in a D-galactose-induced aging mouse model. Evid Based Complement Alternat Med 2012;2012. ID 273435.10.1155/2012/273435Search in Google Scholar PubMed PubMed Central
6. Chen X, Wu G, Huang Z. Structural analysis and antioxidant activities of polysaccharides from cultured Cordyceps militaris. Int J Biol Macromol 2013;58:18–22.10.1016/j.ijbiomac.2013.03.041Search in Google Scholar PubMed
7. Won SY, Park EH. Anti-inflammatory and related pharmacological activities of cultured mycelia and fruiting bodies of Cordyceps militaris. J Ethnopharmacol 2005;96:555–61.10.1016/j.jep.2004.10.009Search in Google Scholar PubMed
8. Cui JD. Biotechnological production and applications of Cordyceps militaris, a valued traditional Chinese medicine. Crit Rev Biotechnol 2015;35:475–84.10.3109/07388551.2014.900604Search in Google Scholar PubMed
9. Jing Y, Cui X, Chen Z, Huang L, Song L, Liu T, et al. Elucidation and biological activities of a new polysaccharide from cultured Cordyceps militaris. Carbohydr Polym 2014;102:288–96.10.1016/j.carbpol.2013.11.061Search in Google Scholar PubMed
10. Lin R, Liu H, Wu S, Pang L, Jia M, Fan K, et al. Production and in vitro antioxidant activity of exopolysaccharide by a mutant, Cordyceps militaris SU5-08. Int J Biol Macromol 2012;51:153–7.10.1016/j.ijbiomac.2012.04.011Search in Google Scholar PubMed
11. Wu J, Ding ZY, Zhang KC. Improvement of exopolysaccharide production by macro-fungus Auricularia auricula in submerged culture. Enzyme Microbial Technol 2006;39:743–9.10.1016/j.enzmictec.2005.12.012Search in Google Scholar
12. Gao H, Gu WY. Optimization of polysaccharide and ergosterol production from Agaricus brasiliensis by fermentation process. Biochemical Eng J 2007;33:202–10.10.1016/j.bej.2006.10.022Search in Google Scholar
13. Kim HO, Yun JW. A comparative study on the production of exopolysaccharides between two entomopathogenic fungi Cordyceps militaris and Cordyceps sinensis in submerged mycelial cultures. J Appl Microbiol 2005;99:728–38.10.1111/j.1365-2672.2005.02682.xSearch in Google Scholar PubMed
14. Park JP, Kim SW, Hwang HJ, Yun JW. Optimization of submerged culture conditions for the mycelial growth and exo-biopolymer production by Cordyceps militaris. Lett Appl Microbiol 2001;33:76–81.10.1046/j.1472-765X.2001.00950.xSearch in Google Scholar PubMed
15. Kim SW, Hwang HJ, Xu CP, Sung JM, Choi JW, Yun JW. Optimization of submerged culture process for the production of mycelial biomass and exo-polysaccharides by Cordyceps militaris C738. J Appl Microbiol 2003;94:120–6.10.1046/j.1365-2672.2003.01754.xSearch in Google Scholar PubMed
16. Cui JD, Zhang BZ. Comparison of culture methods on exopolysaccharide production in the submerged culture of Cordyceps militaris and process optimization. Lett Appl Microbiol 2011;52:123–8.10.1111/j.1472-765X.2010.02987.xSearch in Google Scholar PubMed
17. Yu R, Yang W, Song L, Yan C, Zhang Z, Zhao Y. Structural characterization and antioxidant activity of a polysaccharide from the fruiting bodies of cultured Cordyceps militaris. Carbohydr Polym 2007;70:430–6.10.1016/j.carbpol.2007.05.005Search in Google Scholar
19. Chen R, Jin C, Li H, Liu Z, Lu J, Li S, et al. Ultrahigh pressure extraction of polysaccharides from Cordyceps militaris and evaluation of antioxidant activity. Sep Purif Technol 2014;134:90–9.10.1016/j.seppur.2014.07.017Search in Google Scholar
20. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric method for determinations of sugars and related substances. Anal Chem 1956;28:350–6.10.1021/ac60111a017Search in Google Scholar
21. Wu M, Wu Y, Qu M, Li W, Yan X. Evaluation of antioxidant activities of water-soluble polysaccharides from brown alga Hizikia fusiformis. Sep Purif Technol 2013;56:28–33.10.1016/j.ijbiomac.2013.01.017Search in Google Scholar PubMed
22. Tahmouzi S, Ghodsi M. Optimum extraction of polysaccharides from motherwort leaf and its antioxidant and antimicrobial activities. Carbohydr Polym 2014;112:396–403.10.1016/j.carbpol.2014.06.024Search in Google Scholar PubMed
23. Zheng Y, Li Y, Wang W. Optimization of ultrasonic-assisted extraction and in vitro antioxidant activities of polysaccharides from Trametes orientalis. Carbohydr Polym 2014;111:315–23.10.1016/j.carbpol.2014.04.034Search in Google Scholar PubMed
24. Lin S. Production of exopolysaccharides by submerged mycelial culture of Grifola frondosa TFRI1073 and their antioxidant and antiproliferative activities. World J Microbiol Biotechnol 2011;27:555–61.10.1007/s11274-010-0489-1Search in Google Scholar
25. Yang HL, He GQ. Influence of nutritional conditions on exopolysaccharide production by submerged cultivation of the medicinal fungus Shiraia bambusicola. World J Microbiol Biotechnol 2008;24:2903–7.10.1007/s11274-008-9832-1Search in Google Scholar
26. Kwon JS, Lee JS, Shin WC, Lee KE, Hong EK. Optimization of culture conditions and medium components for the production of mycelial biomass and exo-polysaccharides with Cordyceps militaris in liquid culture. Biotechnol Biopro Eng 2009;14:756–62.10.1007/s12257-009-0024-0Search in Google Scholar
27. Cui JD, Jia SR. Optimization of medium on exopolysaccharides production in submerged culture of Cordyceps militaris. Food Sci Biotechnol 2010;19:1567–71.10.1007/s10068-010-0222-8Search in Google Scholar
28. Xiao JH, Xiao DM, Xiong Q, Liang ZQ, Zhong JJ. Nutritional requirements for the hyperproduction of bioactive exopolysaccharides by submerged fermentation of the edible medicinal fungus Cordyceps taii. Biochemical Eng J 2010;49:241–9.10.1016/j.bej.2009.12.019Search in Google Scholar
29. Xiao JH, Chen DX, Wan WH, Hu XJ, Qi Y, Liang ZQ. Enhanced simultaneous production of mycelia and intracellular polysaccharide in submerged cultivation of Cordyceps jiangxiensis using desirability functions. Process Biochem 2006;41:1887–93.10.1016/j.procbio.2006.03.031Search in Google Scholar
30. Kim SW, Xu CP, Hwang HJ, Choi JW, Kim CW, Yun JW. Production and characterization of exopolysaccharides from an enthomopathogenic fungus Cordyceps militaris NG3. Biotechnol Prog 2003;19:428–35.10.1021/bp025644kSearch in Google Scholar PubMed
31. Shih IL, Tsai KL, Hsieh CY. Effects of culture conditions on the mycelial growth and bioactive metabolite production in submerged culture of Cordyceps militaris. Biochemical Eng J 2007;33:193–201.10.1016/j.bej.2006.10.019Search in Google Scholar
32. Yang CT, Kuo JT, Lin ES. Screening of medium composition for the free radical-scavenging properties by Antrodia cinnamomea. Int J Food Sci Technol 2010;45:305–11.10.1111/j.1365-2621.2009.02136.xSearch in Google Scholar
33. Zhao L, Dong Y, Chen G, Hu Q. Extraction, purification, characterization and antitumor activity of polysaccharides from Ganoderma lucidum. Carbohydr Polym 2010;80:783–9.10.1016/j.carbpol.2009.12.029Search in Google Scholar
34. Ge Y, Duan Y, Fang G, Wang S. Polysaccharides from fruit calyx of Physalis alkekengi var. Francheti: Isolation, purification, structural features and antioxidant activities. Carbohydr Polym 2009;77:188–93.10.1016/j.carbpol.2008.12.020Search in Google Scholar
35. Wu Z. Effect of different drying methods on chemical composition and bioactivity of finger citron polysaccharides. Int J Biol Macromol 2015;76:218–23.10.1016/j.ijbiomac.2015.02.043Search in Google Scholar PubMed
©2017 by De Gruyter
Articles in the same Issue
- Evolutionary Algorithm-Based Multi-objective Control Scheme for Food Drying Process
- Stability and Bioaccessibility of Fucoxanthin in Nanoemulsions Prepared from Pinolenic Acid-contained Structured Lipid
- Functional and Rheological Properties of Piñuela (Bromelia karatas) in Two Ripening Stages.
- Experimental Study on Heat and Mass Transfer of Millet in a Fixed Furnace
- Effects of Processing Treatments on the Antioxidant Properties of Polysaccharide from Cordyceps militaris
- Production and Preliminary Characterization of Antioxidant Polysaccharide by Submerged Culture of Culinary and Medicinal Fungi Cordyceps militaris CICC14013
- Effect of Heat Processing and Ultrasonication Treatment on Custard Apple Peroxidase Activity and Vitamin C
- Response Surface Methodology Approach for Optimization of Extrusion Process of Production of Poly (Hydroxyl Butyrate-Co-Hydroxyvalerate) /Tapioca Starch Blends
- Phenolic Profile and Antioxidant Capacity of Walnut Extract as Influenced by the Extraction Method and Solvent
- Optimization of the Spray-Drying Process for Developing Stingless Bee Honey Powder
Articles in the same Issue
- Evolutionary Algorithm-Based Multi-objective Control Scheme for Food Drying Process
- Stability and Bioaccessibility of Fucoxanthin in Nanoemulsions Prepared from Pinolenic Acid-contained Structured Lipid
- Functional and Rheological Properties of Piñuela (Bromelia karatas) in Two Ripening Stages.
- Experimental Study on Heat and Mass Transfer of Millet in a Fixed Furnace
- Effects of Processing Treatments on the Antioxidant Properties of Polysaccharide from Cordyceps militaris
- Production and Preliminary Characterization of Antioxidant Polysaccharide by Submerged Culture of Culinary and Medicinal Fungi Cordyceps militaris CICC14013
- Effect of Heat Processing and Ultrasonication Treatment on Custard Apple Peroxidase Activity and Vitamin C
- Response Surface Methodology Approach for Optimization of Extrusion Process of Production of Poly (Hydroxyl Butyrate-Co-Hydroxyvalerate) /Tapioca Starch Blends
- Phenolic Profile and Antioxidant Capacity of Walnut Extract as Influenced by the Extraction Method and Solvent
- Optimization of the Spray-Drying Process for Developing Stingless Bee Honey Powder
