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The influence of the configurations of multiple-impeller on canrenone bioconversion using resting cells of Aspergillus ochraceus

  • Juan Huang , Huixuan Zhu , Shimin Guan , Huaixiang Tian EMAIL logo , Chen Chen , Botao Zhang and Shaofeng Rong EMAIL logo
Published/Copyright: May 10, 2023
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International Journal of Chemical Reactor Engineering
From the journal International Journal of Chemical Reactor Engineering Volume 21 Issue 9

Abstract

11 α-Hydroxycanrenone is a key intermediate in the synthesis of eplernone which is a drug that protects the cardiovascular system. It can be obtained by microbial transformation of canrenone using Aspergillus ochraceus. The impeller configuration has a great impact on the microbial transformation efficiency. In this study, three kinds of multiple-impeller including six-blade Rushton turbine (lower) and six-blade Rushton turbine (upper) (RT + RT), six-blade Rushton turbine (lower) and six-arrow blade turbine (upper) (RT + ABT), six-blade Rushton turbine impeller (lower) and six-blade Chemineer CD6 impeller (upper) (RT + CD6) were employed to carry out the microbial conversion process, which was investigated by experiments and computational fluid dynamic (CFD) simulations. The CFD simulation was performed only for the hydrodynamic part of the bioreactor in this article. The results showed that RT + CD6 gave better conversion ratio compared to the other two multiple impellers. It had higher axial flow and better air volume fraction distribution which was benefit for the biotransformation process. A certain amount of cell content should be guaranteed in order to obtain a good substrate conversion (45 % approximately). The final conversion ratio of canrenone was proportional to the content of mycelium at the late stage of conversion, while the content of mycelium at the early stage had a subtle effect. Besides, A. ochraceus resting cells could tolerate the maximum and average shear strain rate in the order of 2598 s−1 and 52 s−1, respectively. The research results provided a guide for the selection of impeller for the biotransformation of canrenone in biopharmaceutical industry.

Keywords: biotransformation; canrenone; computational fluid dynamics; multiple-impellers; resting cells
Corresponding authors: Huaixiang Tian and Shaofeng Rong, Department of Food Science and Technology, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai, 201418, P.R. China; and Department of Biological Engineering, Shanghai Institute of Technology, Shanghai 201418, P.R. China, E-mail: ,

  1. Author contribution: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The research was supported by the Science and Technology Development Fund for Young and Middle-aged Teachers of Shanghai Institute of Technology, Shanghai, China. (ZQ2021-4).

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2022-11-18
Accepted: 2023-04-16
Published Online: 2023-05-10

© 2023 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/ijcre-2022-0219
Keywords for this article
biotransformation; canrenone; computational fluid dynamics; multiple-impellers; resting cells

Articles in the same Issue

  1. Frontmatter
  2. Articles
  3. Eco friendly synthesis of epoxidized palm oleic acid in acidic ion exchange resin
  4. Two-stage adsorber design for malachite green and methylene blue removal using adsorbents derived from banana peel
  5. Modeling and simulation of trickle bed reactors for the purification of 1-butene
  6. Smith-predictor based enhanced Dual-DOF fractional order control for integrating type CSTRs
  7. Enhancement investigation of mass transfer and mixing performance in the static mixers with three twisted leaves
  8. The influence of the configurations of multiple-impeller on canrenone bioconversion using resting cells of Aspergillus ochraceus
  9. De–NO x conversion of selective catalytic reduction system for diesel engine using dual catalyst coated ceramic monoliths
  10. Experimental study on coal-fired flue gas HCl removal by injecting adsorbent into flue duct
  11. Ferrous and manganese oxalate for efficient heterogenous-Fenton degradation of organic pollutants: composite active site and mechanism perception
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