Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues
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Congzhen Shen
,
Yi Tan
,
Lin Li
Abstract
Thiols are important units in amino acids such as cysteine and peptides like glutathione. Development of chemical sensors capable of precise detection of thiols is important in cancer diagnosis and therapy. We have developed novel two-photon fluorescent turn-on probes for selective detection of thiols. The probes displayed excellent sensitivity and low detection limits. The dual-purpose probes have been demonstrated to be suitable for simultaneous imaging and proteome profiling in live cells and tumor tissues. The unique turn-on design endows the probes with excellent selectivity toward thiols in vitro and in situ, and can be further developed to support a thiol-quantification assay.
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 21877050, 22077051, 81803389
Funding source: National Key R&D Program of China
Award Identifier / Grant number: 2019YFC1711000
Funding source: RGC/CRF Equipment
Award Identifier / Grant number: RGC/CRF C5033-19E
Funding source: RGC/ECS
Award Identifier / Grant number: 25301518
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Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
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Research funding: Funding was provided by the National Key R&D Program of China (2019YFC1711000), National Natural Science Foundation of China (21907037, 22077051, 81803389, 81820108029). RGC/CRF Equipment Grant (RGC/CRF C5033-19E), RGC/ECS (25301518).
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Conflict of interest statement: The authors declare no competing financial interest.
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Supplementary Material
The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2021-0189).
© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Highlight: Chemical Biology in Drug Discovery
- Chemical biology in drug discovery
- Rational approaches towards inorganic and organometallic antibacterials
- Epithelial-mesenchymal transition and H2O2 signaling – a driver of disease progression and a vulnerability in cancers
- Covalent fragment-based ligand screening approaches for identification of novel ubiquitin proteasome system modulators
- Artificial metalloenzymes in a nutshell: the quartet for efficient catalysis
- Photochemical protein modification in complex biological environments: recent advances and considerations for future chemical methods development
- Using the yeast three-hybrid system for the identification of small molecule-protein interactions with the example of ethinylestradiol
- Expanded profiling of β-lactam selectivity for penicillin-binding proteins in Streptococcus pneumoniae D39
- Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues
Artikel in diesem Heft
- Frontmatter
- Highlight: Chemical Biology in Drug Discovery
- Chemical biology in drug discovery
- Rational approaches towards inorganic and organometallic antibacterials
- Epithelial-mesenchymal transition and H2O2 signaling – a driver of disease progression and a vulnerability in cancers
- Covalent fragment-based ligand screening approaches for identification of novel ubiquitin proteasome system modulators
- Artificial metalloenzymes in a nutshell: the quartet for efficient catalysis
- Photochemical protein modification in complex biological environments: recent advances and considerations for future chemical methods development
- Using the yeast three-hybrid system for the identification of small molecule-protein interactions with the example of ethinylestradiol
- Expanded profiling of β-lactam selectivity for penicillin-binding proteins in Streptococcus pneumoniae D39
- Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues
