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Artificial metalloenzymes in a nutshell: the quartet for efficient catalysis

  • Paul Ebensperger EMAIL logo and Claudia Jessen-Trefzer ORCID logo EMAIL logo
Published/Copyright: October 18, 2021
Biological Chemistry
From the journal Biological Chemistry Volume 403 Issue 4

Abstract

Artificial metalloenzymes combine the inherent reactivity of transition metal catalysis with the sophisticated reaction control of natural enzymes. By providing new opportunities in bioorthogonal chemistry and biocatalysis, artificial metalloenzymes have the potential to overcome certain limitations in both drug discovery and green chemistry or related research fields. Ongoing advances in organometallic catalysis, directed evolution, and bioinformatics are enabling the design of increasingly powerful systems that outperform conventional catalysis in a growing number of cases. Therefore, this review article collects challenges and opportunities in designing artificial metalloenzymes described in recent review articles. This will provide an equitable insight for those new to and interested in the field.

Keywords: biocatalysis; in vivo catalysis; new-to-nature reactivity; protein engineering
Corresponding authors: Paul Ebensperger and Claudia Jessen-Trefzer, Institute of Organic Chemistry, University of Freiburg, Albertstrasse 21, D-79104 Freiburg i. Br., Germany, E-mail: , (C. Jessen-Trefzer)

Funding source: Evangelisches Studienwerk Villigst

Award Identifier / Grant number: 851250

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: The author thanks “Evangelisches Studienwerk Villigst” (851250) for financial support.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2021-07-23
Accepted: 2021-10-05
Published Online: 2021-10-18
Published in Print: 2022-03-28

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2021-0329
Keywords for this article
biocatalysis; in vivo catalysis; new-to-nature reactivity; protein engineering

Articles in the same Issue

  1. Frontmatter
  2. Highlight: Chemical Biology in Drug Discovery
  3. Chemical biology in drug discovery
  4. Rational approaches towards inorganic and organometallic antibacterials
  5. Epithelial-mesenchymal transition and H2O2 signaling – a driver of disease progression and a vulnerability in cancers
  6. Covalent fragment-based ligand screening approaches for identification of novel ubiquitin proteasome system modulators
  7. Artificial metalloenzymes in a nutshell: the quartet for efficient catalysis
  8. Photochemical protein modification in complex biological environments: recent advances and considerations for future chemical methods development
  9. Using the yeast three-hybrid system for the identification of small molecule-protein interactions with the example of ethinylestradiol
  10. Expanded profiling of β-lactam selectivity for penicillin-binding proteins in Streptococcus pneumoniae D39
  11. Two-photon fluorescent turn-on probes for highly efficient detection and profiling of thiols in live cells and tissues
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