Startseite Lebenswissenschaften SRPK1 promotes cell proliferation and tumor growth of osteosarcoma through activation of the NF-κB signaling pathway
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SRPK1 promotes cell proliferation and tumor growth of osteosarcoma through activation of the NF-κB signaling pathway

  • Yubao Gong ORCID logo EMAIL logo , Chen Yang , Zhengren Wei und Jianguo Liu
Veröffentlicht/Copyright: 29. Dezember 2021
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 403 Heft 7

Abstract

To explore the expression and the functions of SRPK1 in osteosarcoma, we retrieved transcription profiling dataset by array of human bone specimens from patients with osteosarcoma from ArrayExpress (accession E-MEXP-3628) and from Gene Expression Omnibus (accession GSE16102) and analyzed expression level of SRPK1 and prognostic value in human osteosarcoma. Then we examined the effect of differential SRPK1 expression levels on the progression of osteosarcoma, including cell proliferation, cell cycle, apoptosis, and investigated its underlying molecular mechanism using in vitro osteosarcoma cell lines and in vivo nude mouse xenograft models. High expression level of SRPK1 was found in human osteosarcoma tissues and cell lines as compared to the normal bone tissues and osteoblast cells, and predicted poor prognosis of human osteosarcoma. Overexpression of SRPK1 in osteosarcoma U2OS cells led to cell proliferation but inhibition of apoptosis. In contrast, knockdown of SRPK1 in HOS cells impeded cell viability and induction of apoptosis. Moreover, silencing SRPK1 inhibited osteosarcoma tumor growth in nude mice. Mechanistic studies revealed that SRPK1 promoted cell cycle transition in osteosarcoma cells and activation of NF-κB is required for SRPK1 expression and its pro-survival signaling. SRPK1 promoted human osteosarcoma cell proliferation and tumor growth by regulating NF-κB signaling pathway.

Keywords: cell cycle; cell proliferation; NF-κB; osteosarcoma; SRPK1
Corresponding author: Yubao Gong, Department of Orthopedics, The First Hospital of Jilin University, No. 1 Xinmin Street, Changchun 130021, China, E-mail:

  1. Author contributions: Yubao Gong designed the study. Yubao Gong, Chen Yang and Zhengren Wei, performed the experiments. Yubao Gong and Chen Yang contributed to the writing of the manuscript. Yubao Gong, Chen Yang and Jianguo Liu performed the analyses and calculations. All authors read and approved the final manuscript.

  2. Research funding: None declared.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

  4. Conflict of interest statement Ethics approval: This study was approved by the Institutional Animal Care and Use Committee of the First Hospital of Jilin University.

  5. Conflict of interest statement Availability of data and materials: Please contact the corresponding author (Yubao Gong) for data requests.

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Supplementary Material

The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2020-0394).

Received: 2020-12-23
Accepted: 2021-12-20
Published Online: 2021-12-29
Published in Print: 2022-06-27

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

  1. Frontmatter
  2. Review
  3. Chemerin – exploring a versatile adipokine
  4. Research Articles/Short Communications
  5. Protein Structure and Function
  6. Evolutionary adaptation of DHFR via expression of enzyme isoforms with various binding properties and dynamics behavior: a bioinformatics and computational study
  7. Cell Biology and Signaling
  8. SRPK1 promotes cell proliferation and tumor growth of osteosarcoma through activation of the NF-κB signaling pathway
  9. LINC00520 up-regulates SOX5 to promote cell proliferation and invasion by miR-4516 in human hepatocellular carcinoma
  10. Long non-coding RNA FAM66C regulates glioma growth via the miRNA/LATS1 signaling pathway
  11. CERKL alleviates ischemia reperfusion-induced nervous system injury through modulating the SIRT1/PINK1/Parkin pathway and mitophagy induction
https://doi.org/10.1515/hsz-2020-0394
Schlagwörter für diesen Artikel
cell cycle; cell proliferation; NF-κB; osteosarcoma; SRPK1

Artikel in diesem Heft

  1. Frontmatter
  2. Review
  3. Chemerin – exploring a versatile adipokine
  4. Research Articles/Short Communications
  5. Protein Structure and Function
  6. Evolutionary adaptation of DHFR via expression of enzyme isoforms with various binding properties and dynamics behavior: a bioinformatics and computational study
  7. Cell Biology and Signaling
  8. SRPK1 promotes cell proliferation and tumor growth of osteosarcoma through activation of the NF-κB signaling pathway
  9. LINC00520 up-regulates SOX5 to promote cell proliferation and invasion by miR-4516 in human hepatocellular carcinoma
  10. Long non-coding RNA FAM66C regulates glioma growth via the miRNA/LATS1 signaling pathway
  11. CERKL alleviates ischemia reperfusion-induced nervous system injury through modulating the SIRT1/PINK1/Parkin pathway and mitophagy induction
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