Elucidating the anti-biofilm and anti-quorum sensing potential of selenocystine against respiratory tract infections causing bacteria: in vitro and in silico studies

Bharti Patel; Subrata Mishra; Indira K. Priyadarsini; Sirisha L. Vavilala

doi:10.1515/hsz-2020-0375

Article

Elucidating the anti-biofilm and anti-quorum sensing potential of selenocystine against respiratory tract infections causing bacteria: in vitro and in silico studies

Bharti Patel , Subrata Mishra , Indira K. Priyadarsini and Sirisha L. Vavilala

Published/Copyright: March 19, 2021

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From the journal Biological Chemistry Volume 402 Issue 7

Abstract

Bacteria are increasingly relying on biofilms to develop resistance to antibiotics thereby resulting in their failure in treating many infections. In spite of continuous research on many synthetic and natural compounds, ideal anti-biofilm molecule is still not found thereby warranting search for new class of molecules. The current study focuses on exploring anti-biofilm potential of selenocystine against respiratory tract infection (RTI)-causing bacteria. Anti-bacterial and anti-biofilm assays demonstrated that selenocystine inhibits the growth of bacteria in their planktonic state, and formation of biofilms while eradicating preformed-biofilm effectively. Selenocystine at a MIC₅₀ as low as 42 and 28 μg/mL effectively inhibited the growth of Klebsiella pneumonia and Pseudomonas aeruginosa. The antibacterial effect is further reconfirmed by agar cup diffusion assay and growth-kill assay. Selenocystine showed 30–60% inhibition of biofilm formation in K. pneumonia, and 44–70% in P. aeruginosa respectively. It also distorted the preformed-biofilms by degrading the eDNA component of the Extracellular Polymeric Substance matrix. Molecular docking studies of selenocystine with quorum sensing specific proteins clearly showed that through the carboxylic acid moiety it interacts and inhibits the protein function, thereby confirming its anti-biofilm potential. With further validation selenocystine can be explored as a potential candidate for the treatment of RTIs.

Keywords: biofilm eradication; biofilm inhibition; molecular docking; pyocyanin; quorum sensing; selenocystine

Corresponding authors: Indira K. Priyadarsini and Sirisha L. Vavilala, School of Biological and Chemical Sciences, UM DAE Centre for Excellence in Basic Sciences, University of Mumbai, Kalina Campus, Santacruz East, Mumbai400098, India, E-mail: k.indira@cbs.ac.in, sirisha@cbs.ac.in

Funding source: Department of Atomic Energy

Acknowledgments

This work is supported by Department of Atomic Energy, India. The authors are thankful to Dr V.K. Jain, Director, CEBS for constant support and encouragement.

Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.
Research funding: This work is supported by Department of Atomic Energy, India.
Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2020-11-24

Accepted: 2021-03-10

Published Online: 2021-03-19

Published in Print: 2021-06-25

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https://doi.org/10.1515/hsz-2020-0375

Keywords for this article

biofilm eradication; biofilm inhibition; molecular docking; pyocyanin; quorum sensing; selenocystine