Startseite Elucidating the anti-biofilm and anti-quorum sensing potential of selenocystine against respiratory tract infections causing bacteria: in vitro and in silico studies
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Elucidating the anti-biofilm and anti-quorum sensing potential of selenocystine against respiratory tract infections causing bacteria: in vitro and in silico studies

  • Bharti Patel , Subrata Mishra , Indira K. Priyadarsini ORCID logo EMAIL logo und Sirisha L. Vavilala EMAIL logo
Veröffentlicht/Copyright: 19. März 2021
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 402 Heft 7

Abstract

Bacteria are increasingly relying on biofilms to develop resistance to antibiotics thereby resulting in their failure in treating many infections. In spite of continuous research on many synthetic and natural compounds, ideal anti-biofilm molecule is still not found thereby warranting search for new class of molecules. The current study focuses on exploring anti-biofilm potential of selenocystine against respiratory tract infection (RTI)-causing bacteria. Anti-bacterial and anti-biofilm assays demonstrated that selenocystine inhibits the growth of bacteria in their planktonic state, and formation of biofilms while eradicating preformed-biofilm effectively. Selenocystine at a MIC50 as low as 42 and 28 μg/mL effectively inhibited the growth of Klebsiella pneumonia and Pseudomonas aeruginosa. The antibacterial effect is further reconfirmed by agar cup diffusion assay and growth-kill assay. Selenocystine showed 30–60% inhibition of biofilm formation in K. pneumonia, and 44–70% in P. aeruginosa respectively. It also distorted the preformed-biofilms by degrading the eDNA component of the Extracellular Polymeric Substance matrix. Molecular docking studies of selenocystine with quorum sensing specific proteins clearly showed that through the carboxylic acid moiety it interacts and inhibits the protein function, thereby confirming its anti-biofilm potential. With further validation selenocystine can be explored as a potential candidate for the treatment of RTIs.

Keywords: biofilm eradication; biofilm inhibition; molecular docking; pyocyanin; quorum sensing; selenocystine
Corresponding authors: Indira K. Priyadarsini and Sirisha L. Vavilala, School of Biological and Chemical Sciences, UM DAE Centre for Excellence in Basic Sciences, University of Mumbai, Kalina Campus, Santacruz East, Mumbai400098, India, E-mail: ,

Funding source: Department of Atomic Energy

Acknowledgments

This work is supported by Department of Atomic Energy, India. The authors are thankful to Dr V.K. Jain, Director, CEBS for constant support and encouragement.

  1. Author contributions: All the authors have accepted responsibility for the entire content of this submitted manuscript and approved submission.

  2. Research funding: This work is supported by Department of Atomic Energy, India.

  3. Conflict of interest statement: The authors declare no conflicts of interest regarding this article.

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Received: 2020-11-24
Accepted: 2021-03-10
Published Online: 2021-03-19
Published in Print: 2021-06-25

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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  8. Cell Biology and Signaling
  9. Berberine improves dietary-induced cardiac remodeling by upregulating Kruppel-like factor 4-dependent mitochondrial function
  10. Decreased level of miR-1301 promotes colorectal cancer progression via activation of STAT3 pathway
  11. JMJD3-regulated expression of IL-6 is involved in the proliferation and chemosensitivity of acute myeloid leukemia cells
  12. Modulation of recombinant human alpha 1 glycine receptor by flavonoids and gingerols
  13. IL-24 inhibits the malignancy of human glioblastoma cells via destabilization of Zeb1
  14. Glycation of benign meningioma cells leads to increased invasion
  15. Proteolysis
  16. Marked difference in efficiency of the digestive enzymes pepsin, trypsin, chymotrypsin, and pancreatic elastase to cleave tightly folded proteins
https://doi.org/10.1515/hsz-2020-0375
Schlagwörter für diesen Artikel
biofilm eradication; biofilm inhibition; molecular docking; pyocyanin; quorum sensing; selenocystine

Artikel in diesem Heft

  1. Frontmatter
  2. Research Articles/Short Communications
  3. Protein Structure and Function
  4. Structural and kinetic characterization of Porphyromonas gingivalis glutaminyl cyclase
  5. Molecular Medicine
  6. Elucidating the anti-biofilm and anti-quorum sensing potential of selenocystine against respiratory tract infections causing bacteria: in vitro and in silico studies
  7. Carotenoids in Sporidiobolus pararoseus ameliorate diabetic nephropathy in mice through attenuating oxidative stress
  8. Cell Biology and Signaling
  9. Berberine improves dietary-induced cardiac remodeling by upregulating Kruppel-like factor 4-dependent mitochondrial function
  10. Decreased level of miR-1301 promotes colorectal cancer progression via activation of STAT3 pathway
  11. JMJD3-regulated expression of IL-6 is involved in the proliferation and chemosensitivity of acute myeloid leukemia cells
  12. Modulation of recombinant human alpha 1 glycine receptor by flavonoids and gingerols
  13. IL-24 inhibits the malignancy of human glioblastoma cells via destabilization of Zeb1
  14. Glycation of benign meningioma cells leads to increased invasion
  15. Proteolysis
  16. Marked difference in efficiency of the digestive enzymes pepsin, trypsin, chymotrypsin, and pancreatic elastase to cleave tightly folded proteins
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