Abstract
Hydrophobins are fungal proteins that can self-assemble into amphiphilic films at hydrophobic-hydrophilic interfaces. Class I hydrophobin aggregates resemble amyloid fibrils, sharing some features with them. Here, five site-directed mutants of Vmh2, a member of basidiomycota class I hydrophobins, were designed and characterized to elucidate the molecular determinants playing a key role in class I hydrophobin self-assembly. The mechanism of fibril formation proposed for Vmh2 foresees that the triggering event is the destabilization of a specific loop (L1), leading to the formation of a β-hairpin, which in turn generates the β-spine of the amyloid fibril.
Acknowledgments
The authors thank Dr. Alfredo Maria Gravagnuolo for the helpful discussions. This work was supported by a grant from the University Federico II, Naples, Italy (000023_ALTRI_DR_409_2017_Ricerca Ateneo_GIARDINA) ‘Immobilization of ENzymes on hydrophobin-functionalized NAnomaterials, IENA’.
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Supplementary Material:
The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2018-0124).
©2018 Walter de Gruyter GmbH, Berlin/Boston
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Articles in the same Issue
- Frontmatter
- Reviews
- Exploratory cell dynamics: a sense of touch for cells?
- Kallikrein-related peptidases and associated microRNAs as promising prognostic biomarkers in gastrointestinal malignancies
- Sphingolipid metabolism – an ambiguous regulator of autophagy in the brain
- Minireview
- Insulin-like signaling within and beyond metazoans
- Research Articles/Short Communications
- Genes and Nucleic Acids
- Oxidation of 1-N2-etheno-2′-deoxyguanosine by singlet molecular oxygen results in 2′-deoxyguanosine: a pathway to remove exocyclic DNA damage?
- Protein Structure and Function
- Novel approach to quorum quenching: rational design of antibacterials in combination with hexahistidine-tagged organophosphorus hydrolase
- The forkhead domain hinge-loop plays a pivotal role in DNA binding and transcriptional activity of FOXP2
- New clues into the self-assembly of Vmh2, a basidiomycota class I hydrophobin
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- Model construction of Niemann-Pick type C disease in zebrafish
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- Upregulation of Twist is involved in Gli1 induced migration and invasion of hepatocarcinoma cells