Abstract
Hedgehog (Hh) signaling is involved in the progression of hepatocellular carcinoma (HCC), while its detailed mechanisms are not well illustrated. Our present study revealed that the expression of Gli1, while not Gli2 or Gli3, is significantly increased in HCC cell lines and 20/28 (71.4%) HCC tissues as compared with their corresponding controls. Over expression of Gli1 can promote the migration, invasion and epithelial-mesenchymal transition (EMT) of HCC cells. Gli1 can increase the expression of Twist, while not other EMT transcription factors such as Snail, ZEB1 or Slug. Gli1 increases the transcription of Twist while it has no significant effect on the protein or mRNA stability. Chromatin immunoprecipitation-polymerase chain reaction confirms that Gli1 can directly bind to the promoter of Twist, in which the third binding site is essential for Gli1 induced transcription. Collectively, our data suggest that upregulation of Twist is involved in Gli1 induced migration and invasion of HCC cells.
Funding source: National Natural Science Foundation of China
Award Identifier / Grant number: 81702927
Funding statement: The National Natural Science Foundation of China (Funder Id: 10.13039/501100001809, 81702927); the Medical Science and Technology research project of Henan province (201702001); Key Scientific Research Project of Henan Higher Education Institutions of China (18A320038); Joint research fund of the First Affiliated Hospital of Zhengzhou University and the Dalian institute of Chemical Physics, Chinese Academy of Science.
Conflict of interest statement: The authors declare no conflict of interest.
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©2018 Walter de Gruyter GmbH, Berlin/Boston
Articles in the same Issue
- Frontmatter
- Reviews
- Exploratory cell dynamics: a sense of touch for cells?
- Kallikrein-related peptidases and associated microRNAs as promising prognostic biomarkers in gastrointestinal malignancies
- Sphingolipid metabolism – an ambiguous regulator of autophagy in the brain
- Minireview
- Insulin-like signaling within and beyond metazoans
- Research Articles/Short Communications
- Genes and Nucleic Acids
- Oxidation of 1-N2-etheno-2′-deoxyguanosine by singlet molecular oxygen results in 2′-deoxyguanosine: a pathway to remove exocyclic DNA damage?
- Protein Structure and Function
- Novel approach to quorum quenching: rational design of antibacterials in combination with hexahistidine-tagged organophosphorus hydrolase
- The forkhead domain hinge-loop plays a pivotal role in DNA binding and transcriptional activity of FOXP2
- New clues into the self-assembly of Vmh2, a basidiomycota class I hydrophobin
- Membranes, Lipids, Glycobiology
- Model construction of Niemann-Pick type C disease in zebrafish
- Cell Biology and Signaling
- Upregulation of Twist is involved in Gli1 induced migration and invasion of hepatocarcinoma cells
Articles in the same Issue
- Frontmatter
- Reviews
- Exploratory cell dynamics: a sense of touch for cells?
- Kallikrein-related peptidases and associated microRNAs as promising prognostic biomarkers in gastrointestinal malignancies
- Sphingolipid metabolism – an ambiguous regulator of autophagy in the brain
- Minireview
- Insulin-like signaling within and beyond metazoans
- Research Articles/Short Communications
- Genes and Nucleic Acids
- Oxidation of 1-N2-etheno-2′-deoxyguanosine by singlet molecular oxygen results in 2′-deoxyguanosine: a pathway to remove exocyclic DNA damage?
- Protein Structure and Function
- Novel approach to quorum quenching: rational design of antibacterials in combination with hexahistidine-tagged organophosphorus hydrolase
- The forkhead domain hinge-loop plays a pivotal role in DNA binding and transcriptional activity of FOXP2
- New clues into the self-assembly of Vmh2, a basidiomycota class I hydrophobin
- Membranes, Lipids, Glycobiology
- Model construction of Niemann-Pick type C disease in zebrafish
- Cell Biology and Signaling
- Upregulation of Twist is involved in Gli1 induced migration and invasion of hepatocarcinoma cells