Startseite Determination of selenium during pathogenesis of hepatic fibrosis employing hydride generation and inductively coupled plasma mass spectrometry
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Determination of selenium during pathogenesis of hepatic fibrosis employing hydride generation and inductively coupled plasma mass spectrometry

  • Joseph George ORCID logo EMAIL logo
Veröffentlicht/Copyright: 6. Februar 2018
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 399 Heft 5

Abstract

Serum and liver selenium levels were studied during the pathogenesis of N-nitrosodimethylamine (NDMA) induced hepatic fibrosis in rats. The degree of fibrosis was assessed with Masson’s trichrome staining and quantifying collagen content in the liver. Lipid peroxides were measured in blood and liver samples and total glutathione and glutathione peroxidase were assayed in the liver tissue to evaluate oxidative stress. Interleukin-6 (IL-6) and transforming growth factor-β1 (TGF-β1) were measured in the serum. Selenium levels were determined using inductively coupled plasma-mass spectrometry (ICP-MS) after acid digestion and hydride generation of selenium. Serial administrations of NDMA produced well-developed fibrosis and early cirrhosis in the liver with 4-fold increase of total collagen content and deposition of collagen fibers. Blood and hepatic lipid peroxides, serum IL-6 and TGF-β1 were significantly increased. There was significant reduction in hepatic glutathione and glutathione peroxidase levels. Serum and liver selenium were remarkably decreased on all the days studied. The results suggest that decreased selenium and glutathione peroxidase contribute to the impairment of cellular antioxidant defense, which in turn results in oxidative stress and trigger pathogenesis of hepatic fibrosis. The study further demonstrated that ICP-MS with hydride generation technique is a reliable and sensitive method for determination of selenium in biological samples.

Keywords: dimethylnitrosamine; glutathione peroxidase; hepatic fibrosis; ICP-MS; N-nitrosodimethylamine; selenium

Acknowledgments

The author is thankful to Dr. C. K. Mathews, Director, Chemical Group, Indira Gandhi Center for Atomic Research (IGCAR), Kalpakkam, Tamil Nadu, India for his permission to use the PerkinElmer SCIEX ELAN Inductively Coupled Plasma-Mass Spectrometer for the measurement of selenium. The technical assistance of Dr. Lisa George and Ms. S. Vijayalakshmi, Chemical Group, IGCAR, Kalpakkam is gratefully acknowledged. This work was supported by the Indian Council of Medical Research (Funder Id: 10.13039/501100001411), New Delhi, India, by grant no. 3/1/2/3/(9201540)/92-NCD-III to the author.

  1. Conflicts of interest statement: The author does not have any conflicts of interest to declare in connection with this article.

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Received: 2017-10-04
Accepted: 2018-01-11
Published Online: 2018-02-06
Published in Print: 2018-04-25

©2018 Walter de Gruyter GmbH, Berlin/Boston

Artikel in diesem Heft

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  2. Reviews
  3. Generation of superoxide and hydrogen peroxide by side reactions of mitochondrial 2-oxoacid dehydrogenase complexes in isolation and in cells
  4. Update on mitochondria and muscle aging: all wrong roads lead to sarcopenia
  5. Research Articles/Short Communications
  6. Protein Structure and Function
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  8. Membranes, Lipids, Glycobiology
  9. Changes of the peripheral blood mononuclear cells membrane fluidity from type 1 Gaucher disease patients: an electron paramagnetic resonance study
  10. Molecular Medicine
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  12. Cell Biology and Signaling
  13. Molecular determinants of Drosophila immunophilin FKBP39 nuclear localization
  14. The effect of lncRNA HOTAIR on chemoresistance of ovarian cancer through regulation of HOXA7
  15. Novel Techniques
  16. Determination of selenium during pathogenesis of hepatic fibrosis employing hydride generation and inductively coupled plasma mass spectrometry
https://doi.org/10.1515/hsz-2017-0260
Schlagwörter für diesen Artikel
dimethylnitrosamine; glutathione peroxidase; hepatic fibrosis; ICP-MS; N-nitrosodimethylamine; selenium

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Generation of superoxide and hydrogen peroxide by side reactions of mitochondrial 2-oxoacid dehydrogenase complexes in isolation and in cells
  4. Update on mitochondria and muscle aging: all wrong roads lead to sarcopenia
  5. Research Articles/Short Communications
  6. Protein Structure and Function
  7. CRISPR/Cas9-mediated modification of the extreme C-terminus impairs PDGF-stimulated activity of Duox2
  8. Membranes, Lipids, Glycobiology
  9. Changes of the peripheral blood mononuclear cells membrane fluidity from type 1 Gaucher disease patients: an electron paramagnetic resonance study
  10. Molecular Medicine
  11. Aβ42 oligomers impair the bioenergetic activity in hippocampal synaptosomes derived from APP-KO mice
  12. Cell Biology and Signaling
  13. Molecular determinants of Drosophila immunophilin FKBP39 nuclear localization
  14. The effect of lncRNA HOTAIR on chemoresistance of ovarian cancer through regulation of HOXA7
  15. Novel Techniques
  16. Determination of selenium during pathogenesis of hepatic fibrosis employing hydride generation and inductively coupled plasma mass spectrometry
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