Startseite The consequences of deglycosylation of recombinant intra-melanosomal domain of human tyrosinase
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The consequences of deglycosylation of recombinant intra-melanosomal domain of human tyrosinase

  • Monika B. Dolinska und Yuri V. Sergeev EMAIL logo
Veröffentlicht/Copyright: 31. August 2017

Abstract

Tyrosinase, a melanosomal glycoenzyme, catalyzes initial steps of the melanin biosynthesis. While glycosylation was previously studied in vivo, we present three recombinant mutant variants of human tyrosinase, which were obtained using multiple site-directed mutagenesis, expressed in insect larvae, purified and characterized biochemically. The mutagenesis demonstrated the reduced protein expression and enzymatic activity due to possible loss of protein stability and protein degradation. However, the complete deglycosylation of asparagine residues in vitro, including the residue in position 371, interrupts tyrosinase function, which is consistent with a melanin loss in oculocutaneous albinism type 1 (OCA1) patients.

  1. Funding: National Eye Institute (Grant/Award Number: ‘ZIA EY000476-09’)

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Supplemental Material:

The online version of this article offers supplementary material (https://doi.org/10.1515/hsz-2017-0178).


Received: 2017-6-12
Accepted: 2017-8-25
Published Online: 2017-8-31
Published in Print: 2017-12-20

©2018 Walter de Gruyter GmbH, Berlin/Boston

Heruntergeladen am 6.9.2025 von https://www.degruyterbrill.com/document/doi/10.1515/hsz-2017-0178/pdf
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