Startseite Lebenswissenschaften The long non-coding RNA CRNDE promotes cervical cancer cell growth and metastasis
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The long non-coding RNA CRNDE promotes cervical cancer cell growth and metastasis

  • Yuanyuan Meng , Qi Li , Lianwei Li und Rong Ma EMAIL logo
Veröffentlicht/Copyright: 1. Dezember 2017
Biological Chemistry
Aus der Zeitschrift Biological Chemistry Band 399 Heft 1

Abstract

This study was intended to analyze effects of lncRNA CRNDE on cervical cancer cell growth and metastasis. Fifty pairs of cervical cancer tissues and corresponding adjacent tissues were collected. Expressions of long non-coding RNAs (lncRNAs) in tissue samples were detected by microarray analysis. Expression levels of CRNDE in cervical cancer cells and normal cells were detected by qRT-PCR. Cell-counting kit-8 (CCK-8) assay and clone formation assay were utilized to evaluate cell growth. Wound healing assay and Transwell assay were conducted to detect the migratory and invasive capability of cervical cancer cells. The expressions of CRNDE in cervical cancer tissues and cells were higher than those in normal tissues and cells. CCK-8 assay and clone formation assay showed that the knockdown of CRNDE could inhibit the cell proliferation of HeLa and C-33A cells. Wound healing assay indicated that the downregulation of CRNDE expression could suppress the cell migration. The result of a Transwell assay demonstrated that the number of invasion cells reduced in the CRNDE-si group in comparison with the Mock group. LncRNA CRNDE could promote the cell growth and stimulate the metastasis of cervical cancer cells.

Keywords: cervical cancer; invasion; lncRNA CRNDE; migration; proliferation

  1. Ethics approval and consent to participate: All procedures of this research were in accordance with the Declaration of Helsinki and approved by the Review Ethics Committee of Harbin Medical University Cancer Hospital. All patients participated have given consent to this study.

  2. Conflicts of interest: All authors declare that they have no conflict of interest.

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Received: 2017-7-15
Accepted: 2017-8-18
Published Online: 2017-12-1
Published in Print: 2017-12-20

©2018 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hsz-2017-0199
Schlagwörter für diesen Artikel
cervical cancer; invasion; lncRNA CRNDE; migration; proliferation

Artikel in diesem Heft

  1. Frontmatter
  2. Reviews
  3. Maintaining protein composition in cilia
  4. Eremophilane-type sesquiterpenes from fungi and their medicinal potential
  5. How to get rid of mitochondria: crosstalk and regulation of multiple mitophagy pathways
  6. Minireviews
  7. Targeted degradomics in protein terminomics and protease substrate discovery
  8. Brain plasticity, cognitive functions and neural stem cells: a pivotal role for the brain-specific neural master gene |-SRGAP2–FAM72-|
  9. Research Articles/Short Communications
  10. Protein Structure and Function
  11. Domain topology of human Rasal
  12. The consequences of deglycosylation of recombinant intra-melanosomal domain of human tyrosinase
  13. Cell Biology and Signaling
  14. Locally produced xenin and the neurotensinergic system in pancreatic islet function and β-cell survival
  15. The long non-coding RNA CRNDE promotes cervical cancer cell growth and metastasis
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