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Fluorescence and excited state dynamics of the deprotonated Schiff base retinal in proteorhodopsin

  • Elena Bühl , Markus Braun , Andrea Lakatos , Clemens Glaubitz and Josef Wachtveitl EMAIL logo
Published/Copyright: June 16, 2015

Abstract

The UV light absorbing species of proteorhodopsin with deprotonated Schiff base retinal was investigated using steady-state fluorescence and femtosecond pump-probe spectroscopy. Compared to the all-trans retinal with protonated Schiff base, the deprotonated chromophore absorbs at 365 nm and exhibits a blue-shifted fluorescence spectrum. The unusually long-lived excited state decays bi-exponentially with time constants of 8 ps and 130 ps to form a deprotonated 13-cis retinal as the primary photo-product.


Corresponding author: Josef Wachtveitl, Institute of Physical and Theoretical Chemistry, Goethe University Frankfurt, D-60438 Frankfurt/Main, Germany, e-mail:

Acknowledgments

This work has been funded by the DFG via SFB 807 ‘Transport and Communication across Biological Membranes’. We thank Maria Dell for her contributions to the titration experiments.

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Supplemental Material:

The online version of this article (DOI: 10.1515/hsz-2015-0108) offers supplementary material, available to authorized users.


Received: 2015-1-16
Accepted: 2015-6-10
Published Online: 2015-6-16
Published in Print: 2015-9-1

©2015 by De Gruyter

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