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Zingiber officinale (Ginger) hydroalcoholic extract improved avoidance memory in rat model of streptozotocin-induced diabetes by regulating brain oxidative stress

  • Narges Marefati , Tara Abdi , Farimah Beheshti , Farzaneh Vafaee , Maryam Mahmoudabady ORCID logo and Mahmoud Hosseini EMAIL logo
Published/Copyright: October 22, 2021
Hormone Molecular Biology and Clinical Investigation
From the journal Hormone Molecular Biology and Clinical Investigation Volume 43 Issue 1

Abstract

Objectives

Diabetes mellitus associated cognitive impairment is suggested to be due to oxidative stress. Considering the anti-diabetic, antioxidant, antihyperlipidemic, and anti-inflammatory effects of Zingiber officinale, the present study aimed to investigate its effect on memory and oxidative stress factors in streptozotocin (STZ)-induced diabetic rats.

Methods

The rats were allocated into five groups: Control, Diabetic, Diabetic + Ginger 100, Diabetic + Ginger 200, and Diabetic + Ginger 400. Following diabetes induction by STZ (60 mg/kg), 100, 200, or 400 mg/kg Ginger was given for eight weeks. Passive avoidance test (PA) was done and thiol, malondialdehyde (MDA), superoxide dismutase (SOD), and catalase (CAT) measurements were carried out in the brain.

Results

The latency into the dark compartment decreased (p<0.001) and the number of entries and time spent in the dark chamber increased in the Diabetic group compared to the Control (p<0.001 for all). All three doses of extract improved performance of the rats in the PA test (p<0.001 for all). The hippocampal and cortical MDA level was higher (p<0.001) while CAT, SOD, and total thiol were lower (p<0.01–p<0.001) in the Diabetic group than the Control. Treatment with 200 and 400 mg/kg Z. officinale extract reduced hippocampal and cortical MDA (p<0.001) and improved CAT (p<0.001) while, just the dose of 400 mg/kg of the extract increased SOD and total thiol in hippocampal and cortical tissues (p<0.001) compared with Diabetic group.

Conclusions

Z. officinale extract could improve memory by reducing the oxidative stress in STZ-induced diabetes model.

Keywords: diabetes mellitus; memory; oxidative stress; streptozotocin (STZ); Zingiber officinale
Corresponding author: Mahmoud Hosseini, PhD, Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; and Department of Physiology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran, Phone: +98 51 38828565, Fax: +98 51 38828564, E-mail:

Funding source: Mashhad University of Medical Sciences

Award Identifier / Grant number: 980070

  1. Research funding: This study was financially supported by the Vice Presidency of Research, Mashhad University of Medical Sciences (NO: 980070).

  2. Competing interests: We declare that we have no conflict of interest.

  3. Ethical approval: Animal procedures were carried out considering the Guide for the Care and Use of Laboratory Animals and were approved by the Committee on Animal Research of Mashhad University of Medical Sciences. The document number was IR.MUMS.MEDICAL.REC.1398.517.

References

1. Rathmann, W, Giani, G. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:2568–9. https://doi.org/10.2337/diacare.27.10.2568.Search in Google Scholar PubMed

2. Hasanein, P, Shahidi, S. Effects of combined treatment with vitamins C and E on passive avoidance learning and memory in diabetic rats. Neurobiol Learn Mem 2010;93:472–8. https://doi.org/10.1016/j.nlm.2010.01.004.Search in Google Scholar PubMed

3. Collaboration, ERF. Diabetes mellitus, fasting blood glucose concentration, and risk of vascular disease: a collaborative meta-analysis of 102 prospective studies. Lancet 2010;375:2215–22. https://doi.org/10.1016/s0140-6736(10)60484-9.Search in Google Scholar PubMed PubMed Central

4. Tien, R, Kucharczyk, J, Kucharczyk, W. MR imaging of the brain in patients with diabetes insipidus. Am J Neuroradiol 1991;12:533–42.Search in Google Scholar

5. Lupien, SB, Bluhm, EJ, Ishii, DN. Systemic insulin-like growth factor-I administration prevents cognitive impairment in diabetic rats, and brain IGF regulates learning/memory in normal adult rats. J Neurosci Res 2003;74:512–23. https://doi.org/10.1002/jnr.10791.Search in Google Scholar PubMed

6. Bhutada, P, Mundhada, Y, Humane, V, Rahigude, A, Deshmukh, P, Latad, S, et al.. Agmatine, an endogenous ligand of imidazoline receptor protects against memory impairment and biochemical alterations in streptozotocin-induced diabetic rats. Prog Neuro-Psychopharmacol Biol Psychiatry 2012;37:96–105. https://doi.org/10.1016/j.pnpbp.2012.01.009.Search in Google Scholar PubMed

7. Kamal, A, Biessels, GJ, Gispen, WH, Ramakers, GM. Synaptic transmission changes in the pyramidal cells of the hippocampus in streptozotocin-induced diabetes mellitus in rats. Brain Res 2006;1073–1074:276–80. https://doi.org/10.1016/j.brainres.2005.12.070.Search in Google Scholar PubMed

8. Cardoso, S, Santos, MS, Seiça, R, Moreira, PI. Cortical and hippocampal mitochondria bioenergetics and oxidative status during hyperglycemia and/or insulin-induced hypoglycemia. Biochim Biophys Acta (BBA) - Mol Basis Dis 2010;1802:942–51. https://doi.org/10.1016/j.bbadis.2010.07.001.Search in Google Scholar PubMed

9. Gispen, WH, Biessels, GJ. Cognition and synaptic plasticity in diabetes mellitus. Trends Neurosci 2000;23:542–9. https://doi.org/10.1016/s0166-2236(00)01656-8.Search in Google Scholar PubMed

10. Bafadam, S, Beheshti, F, Khodabakhshi, T, Asghari, A, Ebrahimi, B, Sadeghnia, HR, et al.. Trigonella foenum-graceum seed (Fenugreek) hydroalcoholic extract improved the oxidative stress status in a rat model of diabetes-induced memory impairment. Horm Mol Biol Clin Investig 2019;39:1–12. https://doi.org/10.1515/hmbci-2018-0074.Search in Google Scholar PubMed

11. Mousavi, SM, Niazmand, S, Hosseini, M, Hassanzadeh, Z, Sadeghnia, HR, Vafaee, F, et al.. Beneficial effects of Teucrium polium and metformin on diabetes-induced memory impairments and brain tissue oxidative damage in rats. Int J Alzheimer’s Dis 2015;2015:493729. https://doi.org/10.1155/2015/493729.Search in Google Scholar PubMed PubMed Central

12. Tuzcu, M, Baydas, G. Effect of melatonin and vitamin E on diabetes-induced learning and memory impairment in rats. Eur J Pharmacol 2006;537:106–10. https://doi.org/10.1016/j.ejphar.2006.03.024.Search in Google Scholar PubMed

13. Wang, K, Chen, Q, Wu, N, Li, Y, Zhang, R, Wang, J, et al.. Berberine ameliorates spatial learning memory impairment and modulates cholinergic anti-inflammatory pathway in diabetic rats. Front Pharmacol 2019;10:1003. https://doi.org/10.3389/fphar.2019.01003.Search in Google Scholar PubMed PubMed Central

14. Baluchnejadmojarad, T, Roghani, M, Homayounfar, H, Hosseini, M. Beneficial effect of aqueous garlic extract on the vascular reactivity of streptozotocin-diabetic rats. J Ethnopharmacol 2003;85:139–44. https://doi.org/10.1016/s0378-8741(02)00372-0.Search in Google Scholar PubMed

15. Hosseini, M, Shafiee, SM, Baluchnejadmojarad, T. Garlic extract reduces serum angiotensin converting enzyme (ACE) activity in nondiabetic and streptozotocin-diabetic rats. Pathophysiology 2007;14:109–12. https://doi.org/10.1016/j.pathophys.2007.07.002.Search in Google Scholar PubMed

16. Rajaei, Z, Hadjzadeh, MA, Nemati, H, Hosseini, M, Ahmadi, M, Shafiee, S. Antihyperglycemic and antioxidant activity of crocin in streptozotocin-induced diabetic rats. J Med Food 2013;16:206–10. https://doi.org/10.1089/jmf.2012.2407.Search in Google Scholar PubMed

17. Ahmadi, M, Rajaei, Z, Hadjzadeh, MA, Nemati, H, Hosseini, M. Crocin improves spatial learning and memory deficits in the Morris water maze via attenuating cortical oxidative damage in diabetic rats. Neurosci Lett 2017;642:1–6. https://doi.org/10.1016/j.neulet.2017.01.049.Search in Google Scholar PubMed

18. Abdi, T, Mahmoudabady, M, Marzouni, HZ, Niazmand, S, Khazaei, M. Ginger (Zingiber officinale Roscoe) extract protects the heart against inflammation and fibrosis in diabetic rats. Can J Diabetes 2020;45:1–8.10.1016/j.jcjd.2020.08.102Search in Google Scholar PubMed

19. Li, X, Ao, M, Zhang, C, Fan, S, Chen, Z, Yu, L. Zingiberis Rhizoma Recens: a review of its traditional uses, phytochemistry, pharmacology, and toxicology. J Evid Based Complementary Altern Med 2021;2021:6668990. https://doi.org/10.1155/2021/6668990.Search in Google Scholar PubMed PubMed Central

20. Zhu, J, Chen, H. Effects of ginger (Zingiber officinale Roscoe) on type 2 diabetes mellitus and components of the metabolic syndrome: a systematic review and meta-analysis of randomized controlled trials. J Evid Based Complementary Altern Med 2018;2018:5692962.10.1155/2018/5692962Search in Google Scholar PubMed PubMed Central

21. Wang, J, Ke, W, Bao, R, Hu, X, Chen, F. Beneficial effects of ginger Zingiber officinale Roscoe on obesity and metabolic syndrome: a review. Ann N Y Acad Sci 2017;1398:83–98. https://doi.org/10.1111/nyas.13375.Search in Google Scholar PubMed

22. Akhani, SP, Vishwakarma, SL, Goyal, RK. Anti‐diabetic activity of Zingiber officinale in streptozotocin‐induced type I diabetic rats. J Pharm Pharmacol 2004;56:101–5. https://doi.org/10.1211/0022357022403.Search in Google Scholar PubMed

23. Bhandari, U, Pillai, K. Effect of ethanolic extract of Zingiber officinale on dyslipidaemia in diabetic rats. J Ethnopharmacol 2005;97:227–30. https://doi.org/10.1016/j.jep.2004.11.011.Search in Google Scholar PubMed

24. Oludoyin, A, Adegoke, S. Efficacy of ginger (Zingiber officinale Roscoe) extracts in lowering blood glucose in normal and high fat diet-induced diabetic rats. Am J Food Nutr 2014;2:55–8.Search in Google Scholar

25. Li, Y, Tran, VH, Duke, CC, Roufogalis, BD. Preventive and protective properties of Zingiber officinale (ginger) in diabetes mellitus, diabetic complications, and associated lipid and other metabolic disorders: a brief review. Evid base Compl Alternative Med 2012;2012. https://doi.org/10.1155/2012/516870.Search in Google Scholar PubMed PubMed Central

26. Lantz, RC, Chen, G, Sarihan, M, Solyom, A, Jolad, S, Timmermann, B. The effect of extracts from ginger rhizome on inflammatory mediator production. Phytomedicine 2007;14:123–8. https://doi.org/10.1016/j.phymed.2006.03.003.Search in Google Scholar PubMed

27. Shukla, Y, Singh, M. Cancer preventive properties of ginger: a brief review. Food Chem Toxicol 2007;45:683–90. https://doi.org/10.1016/j.fct.2006.11.002.Search in Google Scholar PubMed

28. Azizi-Malekabadi, H, Abareshi, A, Beheshti, F, Marefati, N, Norouzi, F, Soukhtanloo, M, et al.. The effect of captopril on inflammation-induced liver injury in male rats. Toxin Rev 2018;39:275–83. https://doi.org/10.1080/15569543.2018.1517802.Search in Google Scholar

29. Pourganji, M, Hosseini, M, Soukhtanloo, M, Zabihi, H, Hadjzadeh, MA-R. Protective role of endogenous ovarian hormones against learning and memory impairments and brain tissues oxidative damage induced by lipopolysaccharide. Iran Red Crescent Med J 2014;16. https://doi.org/10.5812/ircmj.13954.Search in Google Scholar PubMed PubMed Central

30. Hakimi, Z, Salmani, H, Marefati, N, Arab, Z, Gholamnezhad, Z, Beheshti, F, et al.. Protective effects of carvacrol on brain tissue inflammation and oxidative stress as well as learning and memory in lipopolysaccharide-challenged rats. Neurotox Res 2019;37:965–76. https://doi.org/10.1007/s12640-019-00144-5.Search in Google Scholar PubMed

31. Sadeghian, R, Fereidoni, M, Soukhtanloo, M, Azizi-Malekabadi, H, Hosseini, M. Decreased nitric oxide levels in the hippocampus may play a role in learning and memory deficits in ovariectomized rats treated by a high dose of estradiol. Arq Neuro Psiquiatr 2012;70:874–9. https://doi.org/10.1590/s0004-282x2012001100010.Search in Google Scholar PubMed

32. Marefati, N, Boskabady, MH. The effect of onion (Allium cepa) extract on serum oxidant, antioxidant biomarkers in rat model of asthma. Iran J Allergy, Asthma Immunol 2018;17.Search in Google Scholar

33. Madesh, M, Balasubramanian, K. Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys 1998;35:184–8.Search in Google Scholar

34. Bakhtiari-Dovvombaygi, H, Izadi, S, Zare Moghaddam, M, Hashemzehi, M, Hosseini, M, Azhdari-Zarmehri, H, et al.. Beneficial effects of vitamin D on anxiety and depression-like behaviors induced by unpredictable chronic mild stress by suppression of brain oxidative stress and neuroinflammation in rats. Naunyn-Schmiedeberg’s Arch Pharmacol 2020;394:655–67. https://doi.org/10.1007/s00210-020-02002-0.Search in Google Scholar PubMed

35. Aebi, H. [13] Catalase in vitro. Methods Enzymol 1984;105:121–6. https://doi.org/10.1016/s0076-6879(84)05016-3.Search in Google Scholar PubMed

36. Ghasemi, M, Zendehbad, B, Zabihi, H, Hosseini, M, Hadjzadeh, MA, Hayatdavoudi, P. Beneficial effect of leptin on spatial learning and memory in streptozotocin-induced diabetic rats. Balkan Med J 2016;33:102–7. https://doi.org/10.5152/balkanmedj.2015.15084.Search in Google Scholar PubMed PubMed Central

37. Shahveisi, K, Mousavi, SH, Hosseini, M, Rad, AK, Jalali, SA, Rajaei, Z, et al.. The role of local renin-angiotensin system on high glucose-induced cell toxicity, apoptosis and reactive oxygen species production in PC12 cells. Iran J Basic Med Sci 2014;17:613–21.Search in Google Scholar

38. Zhao, S-S, Yang, W-N, Jin, H, Ma, K-G, Feng, G-F. Puerarin attenuates learning and memory impairments and inhibits oxidative stress in STZ-induced SAD mice. Neurotoxicology 2015;51:166–71. https://doi.org/10.1016/j.neuro.2015.10.010.Search in Google Scholar PubMed

39. Khazaei, H, Pesce, M, Patruno, A. Medicinal plants for diabetes associated neurodegenerative diseases: a systematic review of preclinical studies. Phytother Res 2020;35:1697–718.10.1002/ptr.6903Search in Google Scholar PubMed

40. Pistollato, F, Iglesias, RC, Ruiz, R, Aparicio, S, Crespo, J, Lopez, LD, et al.. Nutritional patterns associated with the maintenance of neurocognitive functions and the risk of dementia and Alzheimer’s disease: a focus on human studies. Pharmacol Res 2018;131:32–43. https://doi.org/10.1016/j.phrs.2018.03.012.Search in Google Scholar PubMed

41. Adetuyi, BO, Farombi, EO. 6-Gingerol, an active constituent of ginger, attenuates lipopolysaccharide-induced oxidation, inflammation, cognitive deficits, neuroplasticity, and amyloidogenesis in rat. J Food Biochem 2021;45:e13660.10.1111/jfbc.13660Search in Google Scholar PubMed

42. Almatroodi, SA, Alnuqaydan, AM, Babiker, AY, Almogbel, MA. 6-Gingerol, a bioactive compound of ginger attenuates renal damage in streptozotocin-induced diabetic rats by regulating the oxidative stress and inflammation. Pharmaceutics 2021;13:317–32. https://doi.org/10.3390/pharmaceutics13030317.Search in Google Scholar PubMed PubMed Central

43. Talebi, M, İlgün, S, Ebrahimi, V, Talebi, M, Farkhondeh, T, Ebrahimi, H, et al.. Zingiber officinale ameliorates Alzheimer’s disease and cognitive impairments: lessons from preclinical studies. Biomed Pharmacother 2021;133:111088. https://doi.org/10.1016/j.biopha.2020.111088.Search in Google Scholar PubMed

44. Kongsui, R, Sriraksa, N, Thongrong, S. The neuroprotective effect of Zingiber cassumunar Roxb. extract on LPS-induced neuronal cell loss and astroglial activation within the hippocampus. BioMed Res Int 2020;2020:1–10. https://doi.org/10.1155/2020/4259316.Search in Google Scholar PubMed PubMed Central

45. Hirano, K, Kubo, M, Fukuyama, Y, Namihira, M. Indonesian ginger (bangle) extract promotes neurogenesis of human neural stem cells through WNT pathway activation. Int J Mol Sci 2020;21:4772. https://doi.org/10.3390/ijms21134772.Search in Google Scholar PubMed PubMed Central

46. Gomar, A, Hosseini, A, Mirazi, N, Gomar, M. Effect of Zingiber Officinale (ginger rhizomes) hydroethanolic extract on hyoscine-induced memory impairment in adult male rats. Int Clin Neurosci J 2015;2:105–10.Search in Google Scholar

47. Olanrewaju, JA, Owolabi, JO. Zingiber officinale ethanolic extract attenuated reserpine-induced depression-like condition and associated hippocampal aberrations in experimental Wistar rats. J Exp Pharmacol 2020;12:439–46. https://doi.org/10.2147/jep.s275260.Search in Google Scholar PubMed PubMed Central

48. Wattanathorn, J, Jittiwat, J, Tongun, T, Muchimapura, S, Ingkaninan, K. Zingiber officinale mitigates brain damage and improves memory impairment in focal cerebral ischemic rat. Evid base Compl Alternative Med 2011;2011:1–8. https://doi.org/10.1155/2011/429505.Search in Google Scholar PubMed PubMed Central

49. Molahosseini, A, Taghavi, M, Taghipour, Z, Shabanizadeh, A, Fatehi, F, Arababadi, MK, et al.. The effect of the ginger on the apoptosis of hippochampal cells according to the expression of BAX and Cyclin D1 genes and histological characteristics of brain in streptozotocin male diabetic rats. Cell Mol Biol 2016;62:1–5. https://doi.org/10.14715/cmb/2016.62.12.1.Search in Google Scholar PubMed

50. Mathew, M, Subramanian, S. In vitro evaluation of anti-Alzheimer effects of dry ginger (Zingiber officinale Roscoe) extract. Indian J Exp Biol 2014;52:606–12.Search in Google Scholar

51. Zeng, GF, Zhang, ZY, Lu, L, Xiao, DQ, Zong, SH, He, JM. Protective effects of ginger root extract on Alzheimer disease-induced behavioral dysfunction in rats. Rejuvenation Res 2013;16:124–33. https://doi.org/10.1089/rej.2012.1389.Search in Google Scholar PubMed

52. El-Akabawy, G, El-Kholy, W. Neuroprotective effect of ginger in the brain of streptozotocin-induced diabetic rats. Ann Anat-Anat Anzeiger 2014;196:119–28. https://doi.org/10.1016/j.aanat.2014.01.003.Search in Google Scholar PubMed

53. Shanmugam, KR, Mallikarjuna, K, Kesireddy, N, Sathyavelu Reddy, K. Neuroprotective effect of ginger on anti-oxidant enzymes in streptozotocin-induced diabetic rats. Food Chem Toxicol 2011;49:893–7. https://doi.org/10.1016/j.fct.2010.12.013.Search in Google Scholar PubMed

Received: 2021-04-07
Accepted: 2021-10-06
Published Online: 2021-10-22

© 2021 Walter de Gruyter GmbH, Berlin/Boston

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https://doi.org/10.1515/hmbci-2021-0033
Keywords for this article
diabetes mellitus; memory; oxidative stress; streptozotocin (STZ); Zingiber officinale

Articles in the same Issue

  1. Frontmatter
  2. Letter to the Editor
  3. Is COVID-19 gender biased?
  4. Opinion Paper
  5. The probable mechanism of reduced androgen level in COVID-19 patients
  6. Original Articles
  7. Effect of Auraptene on angiogenesis in Xenograft model of breast cancer
  8. Zingiber officinale (Ginger) hydroalcoholic extract improved avoidance memory in rat model of streptozotocin-induced diabetes by regulating brain oxidative stress
  9. Role of N-terminal pro-B-type natriuretic peptide and troponin T in predicting right ventricular recovery in myocardial infarction
  10. Is there any role of interleukin-6 and high sensitive C-reactive protein in predicting IVF/ICSI success? A prospective cohort study
  11. Does serum TSH level act as a surrogate marker for psychological stress and cardio-metabolic risk among adolescent and young people?
  12. The effects of thyroid dysfunction on DNA damage and apoptosis in liver and heart tissues of rats
  13. The serum levels of testosterone in coronary artery disease patients; relation to NO, eNOS, endothelin-1, and disease severity
  14. Interaction of high-intensity endurance exercise and nandrolone on cardiac remodeling: role of adipo-cardiac axis
  15. Psoralidin exerts anti-tumor, anti-angiogenic, and immunostimulatory activities in 4T1 tumor‐bearing balb/c mice
  16. Short Communication
  17. COVID-19 pandemic and potential of artificial intelligence
  18. Case Report
  19. A positive urine pregnancy test with haemoperitoneum: misdiagnosed postpartum choriocarcinoma with uterine rupture mimicking ruptured ectopic pregnancy
  20. Review Articles
  21. COVID 19 vaccine in patients of hypercoagulable disorders: a clinical perspective
  22. Synopsis of symptoms of COVID-19 during second wave of the pandemic in India
  23. Clustered regularly interspaced short palindromic repeats, a glimpse – impacts in molecular biology, trends and highlights
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