Evaluation of Y chromosome microdeletions and chromosomal anomalies in infertile men
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Ozlem Oz
Abstract
Objectives
Chromosome anomalies and Y chromosome microdeletions are one of the reasons that can be seen in infertile patients and affect fertility. In this study, it was aimed to determine the frequencies of chromosomal anomalies and Y chromosome microdeletions in primary infertile male patients.
Methods
We included 374 patients with primary infertility in this study. Cytogenetic analysis was performed with the GTG banding technique by using trypsin and Giemsa stain. Y microdeletion analysis was studied by multiplex polymerase chain reaction using 28 Y chromosome-specific sequence-tagged sites.
Results
Chromosomal irregularities were detected in 27 (7.22%) of infertile cases. It was observed that 7 (25.92%) of chromosomal irregularities detected in cases were in autosomal and 20 (%74.08) were in gonosomal chromosomes. The incidence of Y chromosome microdeletion was 1.07% (4/374) and the microdeletions were observed in AZFb, AZFc and AZFd regions. AZFc + AZFd deletion was detected in three patients (0.81%) and AZFb + AZFc + AZFd deletion in one patient (0.26%).
Conclusions
In conclusion, gonosomal chromosome irregularity was higher than autosomal chromosome irregularity in infertile men. The frequency of Y microdeletion has different rates according to some factors such as ethnic differences of patients, patient selection criteria, differences in the number of cases, and methodological aspects.
Acknowledgments
We are grateful to the patients, their cooperation made this work possible.
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Research funding: None declared.
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Author contributions: All authors have accepted responsibility for the entire content of this manuscript and approved its submission.
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Competing interests: Authors state no conflict of interest.
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Ethical approval: This study was approved by the Harran University Clinical Research Ethics Committee and the universal principles of the Helsinki Declaration were applied to the study.
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© 2021 Walter de Gruyter GmbH, Berlin/Boston
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Investigating the relationship between insulin resistance and adipose tissue in a randomized Tehrani population
- Antitumor effects of Auraptene in 4T1 tumor‐bearing Balb/c mice
- Voluntary exercise improves sperm parameters in high fat diet receiving rats through alteration in testicular oxidative stress, mir-34a/SIRT1/p53 and apoptosis
- Assessment of AXL and mTOR genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a
- Is there a relation between serum methylarginine levels and infertility?
- Evaluation of Y chromosome microdeletions and chromosomal anomalies in infertile men
- Threshold values of antibodies to estrogen, progesteron and benzo [a] pyrene as a risk factor for the development of endometriosis
- Altered methylarginine levels after surgery in subjects with multinodular goiter
- Effects of eight weeks exercise training on serum levels of adropin in male volleyball players
- Urinary bisphenol A in women with polycystic ovary syndrome – a possible suppressive effect on steroidogenesis?
- Complementary role of p57kip2 immunostaining in diagnosing hydatidiform mole subtypes
- Short Communications
- Circulating Inhibitory Factor 1 levels in adult patients with Prader–Willi syndrome
- Determinants of serum adiponectin levels: a cross-sectional study
- Case Report
- Prophylactic gonadectomy in 46 XY females; why, where and when?
- Review Article
- Is the beta estradiol receptor receiving enough attention for its metabolic importance in postmenopause?
Artikel in diesem Heft
- Frontmatter
- Original Articles
- Investigating the relationship between insulin resistance and adipose tissue in a randomized Tehrani population
- Antitumor effects of Auraptene in 4T1 tumor‐bearing Balb/c mice
- Voluntary exercise improves sperm parameters in high fat diet receiving rats through alteration in testicular oxidative stress, mir-34a/SIRT1/p53 and apoptosis
- Assessment of AXL and mTOR genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a
- Is there a relation between serum methylarginine levels and infertility?
- Evaluation of Y chromosome microdeletions and chromosomal anomalies in infertile men
- Threshold values of antibodies to estrogen, progesteron and benzo [a] pyrene as a risk factor for the development of endometriosis
- Altered methylarginine levels after surgery in subjects with multinodular goiter
- Effects of eight weeks exercise training on serum levels of adropin in male volleyball players
- Urinary bisphenol A in women with polycystic ovary syndrome – a possible suppressive effect on steroidogenesis?
- Complementary role of p57kip2 immunostaining in diagnosing hydatidiform mole subtypes
- Short Communications
- Circulating Inhibitory Factor 1 levels in adult patients with Prader–Willi syndrome
- Determinants of serum adiponectin levels: a cross-sectional study
- Case Report
- Prophylactic gonadectomy in 46 XY females; why, where and when?
- Review Article
- Is the beta estradiol receptor receiving enough attention for its metabolic importance in postmenopause?
