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Voluntary exercise improves sperm parameters in high fat diet receiving rats through alteration in testicular oxidative stress, mir-34a/SIRT1/p53 and apoptosis

  • Hamed Heydari , Rafighe Ghiasi ORCID logo EMAIL logo , Gholamreza Hamidian , Saber Ghaderpour and Rana Keyhanmanesh ORCID logo EMAIL logo
Published/Copyright: February 9, 2021
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Hormone Molecular Biology and Clinical Investigation
From the journal Hormone Molecular Biology and Clinical Investigation Volume 42 Issue 3

Abstract

Objectives

High fat diet can lead to testicular structural and functional disturbances, spermatogenesis disorders as well as infertility. So, the present investigation was proposed to clarify whether voluntary exercise could prevent high fat diet induced reproductive complications in rats through testicular stress oxidative and apoptosis.

Methods

Forty male Wistar rats were randomly divided into four groups; control (C), voluntary exercise (VE), high fat diet (HFD) and high fat diet and voluntary exercise (VE + HFD) groups. The rats in the VE and VE + HFD groups were accommodated in apart cages that had running wheels and the running distance was assessed daily for 10 weeks. In VE + HFD group, animals were fed with HFD for five weeks before commencing exercise. The sperm parameters, the expressions of testicular miR-34a gene, and P53 and SIRT1 proteins as well as testicular apoptosis were analyzed in all groups.

Results

The results indicated that voluntary exercise in VE + HFD group led to significantly increased GPX and SOD activities, SIRT1 protein expression, sperm parameters, and decreased the expression of miR34a gene and Acp53 protein, and cellular apoptosis index compared to HFD group (p<0.001 to p<0.05). The SOD and catalase activities, SIRT1 protein expression, sperm parameters in VE + HFD group were lower than of those of VE group, however, MDA content, expression of Acp53 protein, apoptosis indexes in VE + HFD group was higher than that of VE group (p<0.001 to p<0.05).

Conclusion

This study revealed that voluntary exercise improved spermatogenesis, in part by decreasing the testicular oxidative stress status, apoptosis through alteration in miR-34a/SIRT1/p53 pathway.

Keywords: apoptosis; mir-34a; oxidative stress; p53; SIRT1; voluntary exercise
Corresponding authors: Dr. Rafighe Giassi, PhD, Assistant Professor and Dr. Rana Keyhanmanesh, MD, PhD, Professor, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; and Department of Physiology, Tabriz University of Medical Sciences, Tabriz 5166614756, Iran, Tel/Fax: +984133364664, E-mail: (R. Ghiasi), E-mail: (R. Keyhanmanesh)

Funding source: Drug Applied Research Center, Tabriz University of Medical Sciences

  1. Research funding: This research was authorized and financially supported by the “Drug Applied Research Center” affiliated to “Tabriz University of Medical Sciences in Tabriz, Iran”.

  2. Author contributions: Rafighe Ghiasi and Rana Keyhanmanesh designed the study; Hamed Heydari, Rafighe Ghiasi, Gholamreza Hamidian, Saber Ghaderpour and Rana Keyhanmanesh contributed to acquisition of data; Rafighe Ghiasi and Rana Keyhanmanesh analyzed data; Hamed Heydari wrote and Rafighe Ghiasi and Rana Keyhanmanesh edited the paper. All authors have accepted responsibility for the entire content of this manuscript and approved its submission.

  3. Competing interests: Authors state no conflict of interest.

  4. Informed consent: Not applicable.

  5. Ethical approval: All experimental procedures were approved by the ethics committee of the animal research of Tabriz University of Medical Sciences, Tabriz, Iran (No: IR.TBZMED.VCR.REC.1397.122).

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Received: 2020-12-04
Accepted: 2021-01-19
Published Online: 2021-02-09

© 2021 Walter de Gruyter GmbH, Berlin/Boston

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Investigating the relationship between insulin resistance and adipose tissue in a randomized Tehrani population
  4. Antitumor effects of Auraptene in 4T1 tumor‐bearing Balb/c mice
  5. Voluntary exercise improves sperm parameters in high fat diet receiving rats through alteration in testicular oxidative stress, mir-34a/SIRT1/p53 and apoptosis
  6. Assessment of AXL and mTOR genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a
  7. Is there a relation between serum methylarginine levels and infertility?
  8. Evaluation of Y chromosome microdeletions and chromosomal anomalies in infertile men
  9. Threshold values of antibodies to estrogen, progesteron and benzo [a] pyrene as a risk factor for the development of endometriosis
  10. Altered methylarginine levels after surgery in subjects with multinodular goiter
  11. Effects of eight weeks exercise training on serum levels of adropin in male volleyball players
  12. Urinary bisphenol A in women with polycystic ovary syndrome – a possible suppressive effect on steroidogenesis?
  13. Complementary role of p57kip2 immunostaining in diagnosing hydatidiform mole subtypes
  14. Short Communications
  15. Circulating Inhibitory Factor 1 levels in adult patients with Prader–Willi syndrome
  16. Determinants of serum adiponectin levels: a cross-sectional study
  17. Case Report
  18. Prophylactic gonadectomy in 46 XY females; why, where and when?
  19. Review Article
  20. Is the beta estradiol receptor receiving enough attention for its metabolic importance in postmenopause?
https://doi.org/10.1515/hmbci-2020-0085
Keywords for this article
apoptosis; mir-34a; oxidative stress; p53; SIRT1; voluntary exercise

Articles in the same Issue

  1. Frontmatter
  2. Original Articles
  3. Investigating the relationship between insulin resistance and adipose tissue in a randomized Tehrani population
  4. Antitumor effects of Auraptene in 4T1 tumor‐bearing Balb/c mice
  5. Voluntary exercise improves sperm parameters in high fat diet receiving rats through alteration in testicular oxidative stress, mir-34a/SIRT1/p53 and apoptosis
  6. Assessment of AXL and mTOR genes expression in medullary thyroid carcinoma (MTC) cell line in relation with over expression of miR-144 and miR-34a
  7. Is there a relation between serum methylarginine levels and infertility?
  8. Evaluation of Y chromosome microdeletions and chromosomal anomalies in infertile men
  9. Threshold values of antibodies to estrogen, progesteron and benzo [a] pyrene as a risk factor for the development of endometriosis
  10. Altered methylarginine levels after surgery in subjects with multinodular goiter
  11. Effects of eight weeks exercise training on serum levels of adropin in male volleyball players
  12. Urinary bisphenol A in women with polycystic ovary syndrome – a possible suppressive effect on steroidogenesis?
  13. Complementary role of p57kip2 immunostaining in diagnosing hydatidiform mole subtypes
  14. Short Communications
  15. Circulating Inhibitory Factor 1 levels in adult patients with Prader–Willi syndrome
  16. Determinants of serum adiponectin levels: a cross-sectional study
  17. Case Report
  18. Prophylactic gonadectomy in 46 XY females; why, where and when?
  19. Review Article
  20. Is the beta estradiol receptor receiving enough attention for its metabolic importance in postmenopause?
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