Community analysis of preservative-treated southern pine (Pinus spp.) using terminal restriction fragment length polymorphism (T-RFLP) analysis. Part 1: Fungal field study
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Grant T. Kirker
Abstract
The effects of chlorothalonil (CTN), butylated hydroxytoluene (BHT), and ammoniacal copper quat (ACQ-C) on the fungal community on southern yellow pine (SYP) were assessed using terminal restriction fragment length polymorphism (T-RFLP) analysis over 15 months. Field stakes, treated with 0.25 and 0.37% ACQ-C, 0.1 and 0.25% CTN, 2% BHT alone, 0.1 and 0.25% CTN combined with 2% BHT, and untreated controls, were installed in two field sites in Mississippi. Stakes were sampled at 90-day intervals and rated for decay damage. Fungal DNA was extracted and amplified by non-specific (total fungi) and specific (Basidiomycete) primers and processes for T-RFLP. a-Diversity (richness and diversity) and b-diversity (similarity between communities) were calculated by means of T-RFLP data. The presence of wood preservatives slowed the initial colonization of field stakes by total fungi, resulting in lower richness and diversity that increased over time; however, preservatives increased the richness and diversity of Basidiomycetes. The b-diversity of treated samples was less similar in the early stages of exposure (3–9 months), but coalesced over time into equilibrium communities that were similar to communities on untreated controls. Basidiomycete species compositions were different among treated samples while control communities shared more than 75% of their species. Correlations were found between depletion of 0.1% CTN and increasing fungal diversity, but no other significant correlations were found.
©2012 by Walter de Gruyter Berlin Boston
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Articles in the same Issue
- Original Papers
- Synthesis of glucose esters from cellulose in ionic liquids
- A new soy flour-polyepoxide adhesive system for making interior plywood
- Hydrophobisation of mechanical pulp fibres with sodium dodecyl sulphate functionalised layered double hydroxide particles
- Effect of pH on lignin analysis by Raman spectroscopy
- Fractionation and characterization of lignin carbohydrate complexes (LCCs) of Eucalyptus globulus in residues left after MWL isolation. Part I: Analyses of hemicellulose-lignin fraction (HC-L)
- Surface and internal micro/ultrastructure of TMP fibres produced during high-intensity refining elucidate the development of pulp and paper properties
- Mechanoradical formation and its effects on birch kraft pulp during the preparation of nanofibrillated cellulose with Masuko refining
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- Community analysis of preservative-treated southern pine (Pinus spp.) using terminal restriction fragment length polymorphism (T-RFLP) analysis. Part 1: Fungal field study
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- Identification of biochemical differences between the sapwood and transition zone in Robinia pseudoacacia L. by differential display of proteins
- Short Notes
- Reduction of formaldehyde emission from light MDF panels by adding chestnut shell flour
- On the structure of the active compound in mass deacidification of paper
- Meetings
- Meetings
- Masthead
- Masthead
