Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part I: Microscopic investigation by POM, UV microscopy, and TOF-SIMS
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Peiming Zheng
Abstract
The lignification process from sapwood (sW) to heartwood (hW) in ray parenchyma cells (Pray) of Pinus densiflora has been analyzed by means of ultraviolet (UV) microscopy, acetyl bromide (CH3COBr) lignin determination, and time-of-flight secondary ion mass spectrometry (TOF-SIMS). The cell wall layers were localized by polarized optical microscopy (POM). POM revealed that Pray have almost no secondary wall in sW and have only the outer layer of secondary wall (S1) in the transition zone (TZ) and hW. UV microscopic observations indicated that the secondary wall of Pray, which is in contact with ray tracheids (Trray), begins to lignify in sW, while the secondary wall of Pray, which is not in contact with Trray, is partially lignified in the TZ. The secondary wall of both types of Pray is completely lignified in hW. The CH3COBr lignin content in sW is slightly lower than that in hW. In the TOF-SIMS measurements, the relative intensities of the secondary ions of guaiacyl-lignin (G-lignin) in the rays in sW are significantly lower than those in hW.
Acknowledgments
This work was supported by the Japan Society for the Promotion of Science (Nos. 21228004, 25252032, and 25114508). We thank Hiroyuki Yamamoto (Graduate School of Bioagricultural Science, Nagoya University, Nagoya, Japan) for support with sample preparation and Ruka Takama (Technical Center, Nagoya University, Nagoya, Japan) for technical support with the TOF-SIMS measurements.
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©2014 by De Gruyter
Articles in the same Issue
- Frontmatter
- Original Articles
- Influence of cellulose supramolecular structure on strength properties of chemical pulp
- Morphological, mechanical, and optical properties of cypress papers
- Determination of ion exchange constants for pairs of metal ions to lignocellulosic materials by column chromatography
- Combustion behavior of oak wood (Quercus mongolica L.) modified by 1,3-dimethylol-4,5-dihydroxyethyleneurea (DMDHEU)
- Synthesis and antifungal activity of camphoric acid-based acylhydrazone compounds
- Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part I: Microscopic investigation by POM, UV microscopy, and TOF-SIMS
- Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part II: Microchemical analysis by laser microdissection and thioacidolysis
- Distribution of lignin in vascular bundles of coconut wood (Cocos nucifera) by cellular UV-spectroscopy and relationship between lignification and tensile strength in single vascular bundles
- Compression properties of vascular boundles and parenchyma of rattan (Plectocomia assamica Griff)
- Properties of wood-plastic composites (WPCs) reinforced with extracted and delignified wood flour
- Anisotropic elastic properties of common ash (Fraxinus excelsior L.)
- Destructive and non-destructive evaluation of seven hardwoods and analysis of data correlation
- Experimental validation of green wood peeling assisted by IR heating – some considerations of the analytical system design
- The influence of felling season and log-soaking temperature on the wetting and phenol formaldehyde adhesive bonding characteristics of birch veneer
- Comparative evaluation of various lignin determination methods on hemicellulose-rich fractions of spruce and birch obtained by pressurized hot-water extraction (PHWE) and subsequent ultrafiltration (UF)
- Conversion between basic density and apparent density at any moisture content in Eucalyptus grandis
- Postpeak residual capacity of nailed connections of a shear wall
- Obituary
- Memorial on Horst H. Nimz (1930–2013)
Articles in the same Issue
- Frontmatter
- Original Articles
- Influence of cellulose supramolecular structure on strength properties of chemical pulp
- Morphological, mechanical, and optical properties of cypress papers
- Determination of ion exchange constants for pairs of metal ions to lignocellulosic materials by column chromatography
- Combustion behavior of oak wood (Quercus mongolica L.) modified by 1,3-dimethylol-4,5-dihydroxyethyleneurea (DMDHEU)
- Synthesis and antifungal activity of camphoric acid-based acylhydrazone compounds
- Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part I: Microscopic investigation by POM, UV microscopy, and TOF-SIMS
- Lignification of ray parenchyma cells in the xylem of Pinus densiflora. Part II: Microchemical analysis by laser microdissection and thioacidolysis
- Distribution of lignin in vascular bundles of coconut wood (Cocos nucifera) by cellular UV-spectroscopy and relationship between lignification and tensile strength in single vascular bundles
- Compression properties of vascular boundles and parenchyma of rattan (Plectocomia assamica Griff)
- Properties of wood-plastic composites (WPCs) reinforced with extracted and delignified wood flour
- Anisotropic elastic properties of common ash (Fraxinus excelsior L.)
- Destructive and non-destructive evaluation of seven hardwoods and analysis of data correlation
- Experimental validation of green wood peeling assisted by IR heating – some considerations of the analytical system design
- The influence of felling season and log-soaking temperature on the wetting and phenol formaldehyde adhesive bonding characteristics of birch veneer
- Comparative evaluation of various lignin determination methods on hemicellulose-rich fractions of spruce and birch obtained by pressurized hot-water extraction (PHWE) and subsequent ultrafiltration (UF)
- Conversion between basic density and apparent density at any moisture content in Eucalyptus grandis
- Postpeak residual capacity of nailed connections of a shear wall
- Obituary
- Memorial on Horst H. Nimz (1930–2013)
