Identification of shorter length lamin A protein in mouse ear cartilage tissue
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Ramamoorthy M. Kalidas
Abstract
Lamin A is an intermediate filament protein which is cleaved by the enzyme, FACE 1 at VTRSY↓L. The cleavage is the final step in the production of the mature lamin A protein. The mature lamin A protein localizes in the inner membrane of the nucleus. The mutation in the lamin A gene causes many diseases, including accelerated aging. It is known that the protein is not expressed in neuronal cells of the brain. Many splicing variants of the lamin A gene have been reported. In this study, the amino acid sequence VTRSY (a penta-peptide repeat) was found in three different sites of the C-terminal end of the lamin A protein, the protein expressed in cells of ear cartilage tissues is shorter than the protein expressed in cells of the skin tissues. Using two lamin A antibodies, it was found that the amino acid sequence between penta-peptide 2 and 3 is missing in lamin A protein that was expressed in the cells of mouse ear cartilage tissue, besides the RT-PCR data confirmed that the corresponding coding sequence between the penta repeat 2 and 3 is intact. Cleavage may occur at the penta-peptide (VTRSY) at site 3 in the lamin A tail of mouse ear cartilage.
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Artikel in diesem Heft
- Biochemical evidence for Ca2+-independent functional activation of hPLSCR1 at low pH
- Catabolism of chondroitin sulfate
- The effect of hypoxia on PGE2-stimulated cAMP generation in HMEC-1
- Is there a connection between inflammation, telomerase activity and the transcriptional status of telomerase reverse transcriptase in renal failure?
- The beta-actin gene promoter of rohu carp (Labeo rohita) drives reporter gene expressions in transgenic rohu and various cell lines, including spermatogonial stem cells
- Molecular machines – a new dimension of biological sciences
- Therapeutic potential of PACAP for neurodegenerative diseases
- A newly isolated yeast as an expression host for recombinant lipase
- Identification of shorter length lamin A protein in mouse ear cartilage tissue
- Relationship among IL-6, LDL cholesterol and lipid peroxidation
- Study strategies for long non-coding RNAs and their roles in regulating gene expression
