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Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris

  • Nguyen Hoang Loc EMAIL logo , Le My Tieu Ngoc , Hoang Tan Quang , Nguyen Duc Huy and Nguyen Ngoc Luong
Published/Copyright: December 17, 2015
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Chemical Papers
From the journal Chemical Papers Volume 70 Issue 3

Abstract

Two genes coding endo-β-1,4-glucanases were cloned from Trichoderma asperellum PQ34 which was isolated from Thua Thien Hue province, Vietnam. The expression of these genes in Pichia pastoris produced two enzymes with molecular masses of approximately 46 kDa (about 42 kDa of enzymes and 4 kDa of signal peptide). The effects of induction time and temperature, inducer concentration, and culture medium on the endo-β-1,4-glucanase activity were investigated. The results showed that the highest total activities of two endo-β-1,4-glucanases were approximately 4.7 × 10-8 kat (from Glu1-TA gene) and 7.3 × 10-8 kat (from Glu2-TA gene) occurred after 4 days of induction using 25 mL L-1 methanol at 30°C when the yeast cells were cultured in a YPL medium.

Keywords: endo-β-1,4-glucanase; expression; Glu1-TA; Glu2-TA; Trichoderma asperellum

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Received: 2015-2-24
Revised: 2015-7-21
Accepted: 2015-9-11
Published Online: 2015-12-17
Published in Print: 2016-3-1

Institute of Chemistry, Slovak Academy of Sciences

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https://doi.org/10.1515/chempap-2015-0210
Keywords for this article
endo-β-1,4-glucanase; expression; Glu1-TA; Glu2-TA; Trichoderma asperellum

Articles in the same Issue

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  4. Cloning and expression of two genes coding endo-β-1,4-glucanases from Trichoderma asperellum PQ34 in Pichia pastoris
  5. Adsorption desulphurisation of dimethyl sulphide using nickel-based Y zeolites pretreated by hydrogen reduction
  6. Equilibrium and kinetics of wetting hydrophobic microporous membrane in sodium dodecyl benzene sulphonate and diethanolamine aqueous solutions
  7. Separation of urea adducts in the analysis of complex mineral fertilisers
  8. Cheese whey tangential filtration using tubular mineral membranes
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